First report of a field outbreak of the oriental eye-fluke, Philophthalmus gralli (Mathis & Leger 1910), in commercially reared ostriches (Struthio camelus) in Zimbabwe.

A total of 17 commercially reared ostriches (Struthio camelus) from Msengi farm, Chinhoyi, Zimbabwe, observed with swollen eyes, severe conjunctivitis and constant lacrimation accompanied by a purulent exudate, were restrained for further clinical examination. Some of the birds were semi-blind with severe loss of body condition. When examined, tiny organisms were observed attached to the nictitating membranes and the conjuctival sacs of both eyes. The organisms were identified as Philophthalmus gralli, the "oriental eye-fluke" and Melanoides tuberculata, a prosobranch snail, was confirmed as the intermediate host through natural and experimental infection. To the best of our knowledge this is the first record of the oriental eye-fluke infection in birds in Zimbabwe and Africa and extends its known geographical range.

A survey on environmental contamination of suburban parks and playgrounds in Harare, Zimbabwe, with canine helminths of zoonotic significance.

In an attempt to assess the possible risk to humans of soil-transmitted canine helminths of of zoonotic significance, 161 faecal samples and 81 soil samples were collected from 6 public parks and playgrounds in Harare between March and June 1998 and examined for nematode ova. Of the 161 faecal samples collected, 17.4% were positive for Ancylostoma sp. ova and 5.6% were positive for T. canis ova. No other nematode species ova were found. Over 50% of the faecal samples positive for Ancylostoma sp. ova were 'moist', and this suggests that the moisture content of faeces contributes to the development and survival of this parasite in the environment. Only 3 of the 81 soil samples collected were positive for T. canis. The low levels of contamination of public playgrounds and parks with T. canis ova suggests that environmental contamination might not be important in the aetiology of human toxocarosis in Harare.

Genital tract infections in sexually active women in Barbados.

Ninety-eight women attending three different clinics were prospectively studied for the presence of genital tract infections, including Chlamydia trachomatis. Of these 98 women, 35 were presenting to a polyclinic with symptoms of genital tract infection, 55 were attending an antenatal clinic for their first visit, and 8 referred to a colposcopy clinic because of an abnormal Papanicolaou smear were included. Gonorrhoea was detected in one patient, syphilis in two, and Trichomonas vaginalis in six. Candida albicans and Chlamydia trachomatis were each detected in 18 patients, while the most common condition was bacterial vaginosis, detected in 35 patients. The prevalence of these infections was lowest in patients referred for colposcopy and highest in the women attending the antenatal clinic. Chlamydia trachomatis was the most common sexually-transmitted pathogen detected in this population. These data emphasise the need for an aggressive approach to the diagnosis and treatment of chlamydial infection in females.

Comparison of 15N-labelled glycine, aspartate, valine and leucine for measurement of whole-body protein turnover.

1. Whole-body protein turnover was measured in rats by constant infusion of 15N-labelled glycine, aspartate, valine and leucine and measuring the enrichment of hepatic and renal urea and ammonia nitrogen. 2. The values obtained with [15N]glycine were comparable with values reported with methods based on different assumptions. 3. [15N]Aspartate gave rise to an increased enrichment of urea and ammonia and hence to lower protein-turnover rates. 4. [15N]Valine and [15N]leucine gave low enrichments of nitrogenous end products and hence to high protein-turnover rates. 5. All 15N-labelled amino acids are not equally suitable for measuring whole-body protein turnover by the end-product method. The relative amounts of 15N going to the end products can be predicted from the known individual metabolism of aspartate and the branched-chain amino acids.