New bone-generative effect of Salvia officinalis L. in the expanded midpalatal suture : An in vivo and in vitro study.

PURPOSE: The aims of this study were to evaluate the effects of systemic administration of Salvia officinalis Linnaeus (L.) leaf extract on new bone formation in the expanded premaxillary suture in rats in vivo and to examine the antioxidant effects and phenolic profile of Salvia officinalis (SO) leaf and root extracts in vitro. METHODS: Fourteen male Sprague Dawley rats were allocated to two groups: SO group (n = 7) and control group (n = 7). An open-loop spring was attached to the upper incisors of each rat to expand the premaxillae. A 5-day expansion period followed by a 12-day retention period was observed. The rats in the SO group received systemic administration of 20 mg SO/kg/day via the orogastric route for 17 days. Histomorphometric examinations were carried out to examine the amount of new bone formation, number of capillaries, and intensity of inflammatory cell response. Immunohistochemical analysis was conducted to examine the number of osteoblasts and osteoclasts. Leaf and root extracts of SO were also analyzed for antioxidant activity and phenolic compounds in vitro. RESULTS: Statistical analysis showed that the following were higher in the SO group than in the control group: new bone formation, number of osteoblasts and osteoclasts, intensity of inflammatory cell response (neutrophils, lymphocytes, and macrophages), and number of capillaries. The major compound identified in SO leaf extract was rosmarinic acid, while luteolin derivatives, salvianolic acid F, and medioresinol were also present. CONCLUSIONS: Salvia officinalis L. from leaf extract provided antioxidant effects and stimulated enhanced new bone formation in the expanded midpalatal suture after maxillary expansion in rats.

Effects of different settings for 940 nm diode laser on expanded suture in rats.

OBJECTIVES: To evaluate the effects of the Indium Gallium Arsenide Phosphoride (InGaAsP) diode laser at different energy levels on orthopedically expanded midpalatal sutures of rats. MATERIALS AND METHODS: Eighty Wistar rats were randomly divided into four groups: a control group and low-, moderate-, and high-level laser groups with amounts of energy irradiated at 0 J, 18 J, 42 J, and 60 J, respectively. Each group was divided into two subgroups (n = 10) according to the schedule of sacrifice (7 and 21 days). Laser application (940 ± 10 nm, 0.1 W) was completed twice weekly until sacrifice. The number of osteoblasts (OB), osteocytes (OC), and vessels (V); area of connective tissue (CT); inflammation (IN); and newly formed bone (NB); as well as the ratio of newly formed bone to the total bone area (N/T) were evaluated statistically at a significance level of P < .05. RESULTS: For the low-level laser group, OB, NB, and N/T were significantly higher, and CT was lower, on both the 7th and 21st days. The amount of OC was significantly higher in the low-level laser group compared with the control group on the 7th day and the control and high-level laser groups on the 21st day. The IN was significantly higher for the high-level group on the 21st day compared with other groups. Both the moderate-level and high-level laser groups possessed fewer vessels than the low-level laser group on the 21st day. CONCLUSIONS: The InGaAsP laser at the low dosage induced a favorable effect on bone formation in the orthopedically expanded midpalatal suture of rats.