Maladaptive coping, victimization, and recidivism among Japanese adolescents and emerging adults.

BACKGROUND: Substance use and victimization are known to be related to juvenile recidivism. Self-harm, a factor that commonly accompanies substance use and victimization, is not known to be related to said recidivism but may be so in a welfare-oriented juvenile justice system as found in Japan. OBJECTIVE: We examine the extent to which maladaptive coping, comprising substance use and self-harm, increases the rate of persistence in correctional institutions in light of other well-replicated factors of youth recidivism. The study, too, investigates the role of maladaptive coping in explaining the impact of victimization on correctional recidivism. METHODS: We draw from a sample of 348 adolescents and emerging adults, between ages 12-19 years, who were initially detained at a Juvenile Classification Home and followed-up for an average of 3.35 years. RESULTS: Findings indicate that maladaptive coping is significantly related to persistence in the system, although history of probationary supervision and gang membership also were significant explanatory factors. In addition, the direct effect of victimization was larger than the indirect effect of victimization through maladaptive coping. CONCLUSIONS: Unlike previous studies, self-harm is significantly related to recidivism. This suggests that recidivism reflects a need for help more so than for punishment. The wider implications are that juvenile justice systems characterized as punitive seem outdated in managing detained young people as they lack adequate prevention supports.

Involvement of microRNA-224 in cell proliferation, migration, invasion, and anti-apoptosis in hepatocellular carcinoma.

BACKGROUND AND AIM: Changes in microRNA (miRNA) expression have been detected in a broad range of biological processes including cancer. Here we determined the role of miRNA dysregulation in hepatocellular carcinoma (HCC). METHODS: We investigated the expression of nine cancer-related miRNAs in HCC. Among these, miR-224 was the most significantly uprgulated in HCC tissues (n = 18), compared with normal (n = 9) and HCC adjacent non-tumorous liver tissues (n = 18). After leading-in currently reported gene targets from Sanger miRBase, we characterized the expression profiles of target genes of miR-224 using cDNA microarray. The altered expression was subsequently validated by real-time polymerase chain reaction and Western blot. The phenotypic changes by miR-224 expression were identified by cell viability, apoptosis, and in vitro scratch assays. RESULTS: The microarray analysis and miRNA target prediction analysis allowed the identification of significant changes in 68 putative gene targets after overexpression of miR-224. The high-ranking genes CDC42, CDH1, PAK2, BCL-2, and MAPK1 were confirmed as important targets of miR-224 and involvement in hepatocarcinogenesis. Overexpression of miR-224 significantly in Hek293 and Huh7 cells altered the expression levels of CDC42, CDH1, PAK2, and BCL-2 at both mRNA and protein levels. Similar changes in the expression of the same genes were also observed in HCC tissues. Via functional analyses, cell proliferation, migration and anti-apoptosis were proved to be affected by miR-224 expression. CONCLUSION: The results suggest that miR-224 plays a role in cell proliferation, migration, invasion, and anti-apoptosis in HCC by directly binding to its gene targets, implicating this RNA in HCC development and progression.