ABSTRACT
In this study, chitin extraction from shrimp shell powder (SSP) using locally isolated Paenibacillus jamilae BAT1 (GenBank: MN176658), the preparation of chitosan from the extracted chitin, and the characterization and biological activity (antimicrobial and antioxidant) of the prepared chitosan (PC) were investigated. It was determined that P. jamilae BAT1 did not have chitinase activity but showed high protease activity and protein removal potential. Optimum pH, shell concentration and incubation time for deproteinization were determined as 7.0, 60 g/L and 4 days, respectively. Addition of KH2PO4 or MgSO4 did not affect chitin extraction and deproteinization yield. The maximum yields of deproteinization, demineralization and chitin extraction yields were 87.67, 41.95 and 24.5%, respectively. The viscosity-average molecular weight of PC was determined as 1.41 × 105 g/mol. The deacetylation degree of PC (86%) was found to be higher that of commercial chitosan (CC) (78%). DPPH scavenging activity of PC (IC50 0.59 mg/mL) was higher than that of CC (IC50 3.72 mg/mL). PC was found to have higher antimicrobial activity against the bacteria E. coli and S. aureus and the yeast C. albicans when compared to CC. This is the first study on the use of the bacterium P. jamilae in biological chitin extraction.
