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1.
Dev Dyn ; 253(3): 312-332, 2024 Mar.
Article in English | MEDLINE | ID: mdl-37776236

ABSTRACT

INTRODUCTION: Primary cilia play pivotal roles in the patterning and morphogenesis of a wide variety of organs during mammalian development. Here we examined murine foregut septation in the cobblestone mutant, a hypomorphic allele of the gene encoding the intraflagellar transport protein IFT88, a protein essential for normal cilia function. RESULTS: We reveal a crucial role for primary cilia in foregut division, since their dramatic decrease in cilia in both the foregut endoderm and mesenchyme of mutant embryos resulted in a proximal tracheoesophageal septation defects and in the formation of distal tracheo(broncho)esophageal fistulae similar to the most common congenital tracheoesophageal malformations in humans. Interestingly, the dorsoventral patterning determining the dorsal digestive and the ventral respiratory endoderm remained intact, whereas Hedgehog signaling was aberrantly activated. CONCLUSIONS: Our results demonstrate the cobblestone mutant to represent one of the very few mouse models that display both correct endodermal dorsoventral specification but defective compartmentalization of the proximal foregut. It stands exemplary for a tracheoesophageal ciliopathy, offering the possibility to elucidate the molecular mechanisms how primary cilia orchestrate the septation process. The plethora of malformations observed in the cobblestone embryo allow for a deeper insight into a putative link between primary cilia and human VATER/VACTERL syndromes.


Subject(s)
Ciliopathies , Hedgehog Proteins , Humans , Animals , Mice , Hedgehog Proteins/genetics , Cilia , Alleles , Disease Models, Animal , Mammals
2.
Blood ; 132(25): 2670-2683, 2018 12 20.
Article in English | MEDLINE | ID: mdl-30333121

ABSTRACT

Forkhead box class O1 (FOXO1) acts as a tumor suppressor in solid tumors. The oncogenic phosphoinositide-3-kinase (PI3K) pathway suppresses FOXO1 transcriptional activity by enforcing its nuclear exclusion upon AKT-mediated phosphorylation. We show here abundant nuclear expression of FOXO1 in Burkitt lymphoma (BL), a germinal center (GC) B-cell-derived lymphoma whose pathogenesis is linked to PI3K activation. Recurrent FOXO1 mutations, which prevent AKT targeting and lock the transcription factor in the nucleus, are used by BL to circumvent mutual exclusivity between PI3K and FOXO1 activation. Using genome editing in human and mouse lymphomas in which MYC and PI3K cooperate synergistically in tumor development, we demonstrate proproliferative and antiapoptotic activity of FOXO1 in BL and identify its nuclear localization as an oncogenic event in GC B-cell-derived lymphomagenesis.


Subject(s)
B-Lymphocytes , Burkitt Lymphoma , Cell Nucleus , Cell Transformation, Neoplastic , Forkhead Box Protein O1 , Germinal Center , Animals , B-Lymphocytes/metabolism , B-Lymphocytes/pathology , Burkitt Lymphoma/genetics , Burkitt Lymphoma/metabolism , Burkitt Lymphoma/pathology , Cell Line, Tumor , Cell Nucleus/genetics , Cell Nucleus/metabolism , Cell Nucleus/pathology , Cell Transformation, Neoplastic/genetics , Cell Transformation, Neoplastic/metabolism , Cell Transformation, Neoplastic/pathology , Forkhead Box Protein O1/genetics , Forkhead Box Protein O1/metabolism , Gene Editing , Germinal Center/metabolism , Germinal Center/pathology , Humans , Mice , Phosphatidylinositol 3-Kinases , Proto-Oncogene Proteins c-akt/genetics , Proto-Oncogene Proteins c-akt/metabolism
3.
Neurogenesis (Austin) ; 3(1): e1248206, 2016.
Article in English | MEDLINE | ID: mdl-28090543

ABSTRACT

Midbrain dopaminergic (mDA) neurons are generated in the ventral midbrain floor plate depending on Sonic Hedgehog (SHH) signaling for induction. Primary cilia transduce canonical SHH signals. Loss of intraflagellar transport protein IFT88, essential for ciliary function, disrupts SHH signaling in the ventral midbrain and results in the reduction in mDA progenitors and neurons. We investigate whether conditional inactivation of the kinesin motor protein KIF3A recapitulates phenotypes observed in conditional Ift88 mutants. Conditional Kif3a inactivation reduced the mDA progenitor domain size, but did not result in mDA neuron reduction, most likely because of a delayed loss of cilia and delayed inactivation of SHH signaling. We thereby define a precise spatiotemporal window within which primary cilia-dependent SHH signaling determines mDA fate.

4.
Dev Biol ; 409(1): 55-71, 2016 Jan 01.
Article in English | MEDLINE | ID: mdl-26542012

ABSTRACT

Midbrain dopaminergic (mDA) neurons modulate various motor and cognitive functions, and their dysfunction or degeneration has been implicated in several psychiatric diseases. Both Sonic Hedgehog (Shh) and Wnt signaling pathways have been shown to be essential for normal development of mDA neurons. Primary cilia are critical for the development of a number of structures in the brain by serving as a hub for essential developmental signaling cascades, but their role in the generation of mDA neurons has not been examined. We analyzed mutant mouse lines deficient in the intraflagellar transport protein IFT88, which is critical for primary cilia function. Conditional inactivation of Ift88 in the midbrain after E9.0 results in progressive loss of primary cilia, a decreased size of the mDA progenitor domain, and a reduction in mDA neurons. We identified Shh signaling as the primary cause of these defects, since conditional inactivation of the Shh signaling pathway after E9.0, through genetic ablation of Gli2 and Gli3 in the midbrain, results in a phenotype basically identical to the one seen in Ift88 conditional mutants. Moreover, the expansion of the mDA progenitor domain observed when Shh signaling is constitutively activated does not occur in absence of Ift88. In contrast, clusters of Shh-responding progenitors are maintained in the ventral midbrain of the hypomorphic Ift88 mouse mutant, cobblestone. Despite the residual Shh signaling, the integrity of the mDA progenitor domain is severely disturbed, and consequently very few mDA neurons are generated in cobblestone mutants. Our results identify for the first time a crucial role of primary cilia in the induction of mDA progenitors, define a narrow time window in which Shh-mediated signaling is dependent upon normal primary cilia function for this purpose, and suggest that later Wnt signaling-dependent events act independently of primary cilia.


Subject(s)
Cilia/metabolism , Dopaminergic Neurons/metabolism , Embryo, Mammalian/cytology , Hedgehog Proteins/metabolism , Mesencephalon/cytology , Mesencephalon/embryology , Neurogenesis , Animals , Cilia/ultrastructure , Gene Expression Profiling , Gene Expression Regulation, Developmental , Kruppel-Like Transcription Factors/metabolism , Mice , Mutation/genetics , Nerve Tissue Proteins/metabolism , Neurogenesis/genetics , Neuroglia/metabolism , Phenotype , Receptors, G-Protein-Coupled/metabolism , Signal Transduction/genetics , Smoothened Receptor , Stem Cells/cytology , Stem Cells/metabolism , Tumor Suppressor Proteins/metabolism , Wnt Proteins/metabolism , Zinc Finger Protein Gli2 , Zinc Finger Protein Gli3
5.
Mech Dev ; 130(6-8): 373-80, 2013.
Article in English | MEDLINE | ID: mdl-23085524

ABSTRACT

With a microtubule-based axoneme supporting its plasma membrane-ensheathed projection from the basal body of almost all cell types in the human body, and present in only one copy per cell, the primary cilium can be considered an organelle sui generis. Although it was first observed and recorded in histological studies from the late 19th century, the tiny structure was essentially forgotten for many decades. In the past ten years, however, scientists have turned their eyes once again upon primary cilia and realized that they are very important for the development of almost all organs in the mammalian body, especially those dependent upon the signaling from members Hedgehog family, such as Indian and Sonic hedgehog. In this review, we outline the roles that primary cilia play in forebrain development, not just in the crucial transduction of Sonic hedgehog signaling, but also new results showing that cilia are important for cell cycle progression in proliferating neural precursors. We will focus upon cerebral cortex development but will also discuss the importance of cilia for the embryonic hippocampus, olfactory bulb, and diencephalon.


Subject(s)
Cerebral Cortex/embryology , Cilia/physiology , Hedgehog Proteins/metabolism , Hippocampus/embryology , Olfactory Bulb/embryology , Olfactory Receptor Neurons/physiology , Animals , Cerebral Cortex/cytology , Cerebral Cortex/physiology , Embryo, Mammalian , Gene Expression Regulation, Developmental , Hedgehog Proteins/genetics , Hippocampus/cytology , Hippocampus/physiology , Humans , Olfactory Bulb/cytology , Olfactory Bulb/physiology , Olfactory Receptor Neurons/cytology , Olfactory Receptor Neurons/growth & development , Organogenesis , Signal Transduction
6.
Cell Tissue Res ; 345(1): 21-40, 2011 Jul.
Article in English | MEDLINE | ID: mdl-21638207

ABSTRACT

The primary cilium is a small microtubule-based organelle projecting from the plasma membrane of practically all cells in the mammalian body. In the past 8 years, a flurry of papers has indicated a crucial role of this long-neglected organelle in the development of a wide variety of organs, including derivatives of all three germ layers. A common theme of these studies is the critical dependency of signal transduction of the Hedgehog pathway upon functionally intact cilia to regulate organogenesis. Another common theme is the role that the cilium plays, not necessarily in the determination of the embryonic anlagen of these organs, although this too occurs but rather in the proliferation and morphogenesis of the previously determined organ. We outline the various organ systems that are dependent upon primary cilia for their proper development and we discuss the cilia-dependent roles that Sonic and Indian Hedgehog play in these processes. In addition and most importantly for the field, we discuss the controversial involvement of another major developmental pathway, Wnt signaling, in cilia-dependent organogenesis.


Subject(s)
Cilia/metabolism , Hedgehog Proteins/metabolism , Organogenesis , Animals , Neoplasms/metabolism , Signal Transduction , Wnt Proteins/metabolism
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