The application of miniplex primer sets in the analysis of degraded DNA from human skeletal remains.

A new set of multiplexed PCR primers has been applied to the analysis of human skeletal remains to determine their efficacy in analyzing degraded DNA. These primer sets, known as Miniplexes, produce shorter amplicons (50-280 base pairs (bp)) than standard short tandem repeat (STR) kits, but still utilize the 13 CODIS STR loci, providing results that are searchable on national DNA databases. In this study, a set of 31 different human remains were exposed to a variety of environmental conditions, extracted, and amplified with commercial and Miniplex DNA typing kits. The amplification efficiency of the Miniplex sets was then compared with the Promega PowerPlex 16 system. Sixty-four percent of the samples generated full profiles when amplified with the Miniplexes, while only 16% of the samples generated full profiles with the Powerplex 16 kit. Complete profiles were obtained for 11 of the 12 Miniplex loci with amplicon sizes less than 200 bp. These data suggest smaller PCR amplicons may provide a useful alternative to mitochondrial DNA for anthropological and forensic analysis of degraded DNA from human skeletal remains.

Geographical height variation among Ohio Caucasian male convicts born 1780-1849.

To more completely answer questions regarding health in the past, social scientists are utilizing previously underutilized data sources. This study focuses on one such source: penitentiary records, in order to examine geographical variation in height from a sample of 2554 male Caucasian convicts aged 23-45 years. Data collected include height, age, ancestry, nationality, and year and place of birth. Birth places were divided into five geographical areas corresponding roughly to the United States census geography divisions. Average stature for this sample was 68.4+/-2.5 in. (173.7+/-6.4 cm). Regression analyses on height, birth cohort, ancestry, nationality, and place of birth indicate significant but small differences in height across geographical areas. No significant differences were found due to birth cohort.

Variation in the human cervical neural canal.

BACKGROUND CONTEXT: The dimensions of the cervical spinal canal can impact the likelihood of an individual suffering longtime effects from a spinal neck injury as well as influence recovery time. Most studies have used radiographic studies to compare differences in the neural canal, but few have examined skeletal populations to determine variation in the neural canal dimensions without the presence of soft tissue. PURPOSE: To analyze variation seen in the cervical neural canal (anterior-posterior and transverse diameters) with respect to sex and ancestry and to define cervical canal narrowing in the sample. STUDY DESIGN: Observational. METHODS: Measurements of the anterior-posterior (sagittal) (CAP) and transverse (CTR) diameters were taken from 321 individual skeletons. Comparisons were made between males and females and individuals belonging to different ancestral (racial) groups. RESULTS: CAP was narrowest at the C4 level for African-Americans and at C6 for Caucasians. CTR was narrowest at the C2/C3 level for all groups. Statistical analyses indicated that significant differences in cervical canal dimensions are due first to sexual dimorphism and then to ancestry. CONCLUSIONS: Significant variation in cervical canal dimensions precludes usage of universal definitions to determine spinal stenosis in individuals; definitions should be according to sex and ancestry.

Human identification from the ankle with pre- and postsurgical radiographs.

Fifty-four sets of presurgical ("premortem") and postsurgical ("postmortem") foot and ankle radiographs were retrospectively evaluated to simulate a postmortem identification. The entire foot and ankle was examined in a previous study. The present study evaluates only the ankle for positive identification. Results are consistent with our earlier investigation of pre- and postsurgical foot and ankle radiographic comparisons and indicate that surgical intervention with subsequent healing does not preclude positive identification. However, the ankle contains fewer skeletal features unique to an individual than does the entire foot. Hence, the ankle may be less useful than the foot for establishing positive identification from radiographic comparisons.

Using pre- and post-surgical foot and ankle radiographs for identification.

An evaluation of the effect of surgical intervention on foot and ankle radiographic comparisons was performed. In this study, 34 sets of pre-surgical ("premortem") and post-surgical ("postmortem") foot and ankle radiographs were retrospectively evaluated simulating a postmortem identification. In each radiographic set, the films were separated by a surgical event to reproduce the effects of an alteration in the anatomy. The radiographs included both matches and mismatches. This study also presents a numerical representation of the reliability of a radiographic match following a surgical procedure. Results indicate that surgical intervention with subsequent healing does not preclude positive identification in foot and ankle radiographic comparisons.