ABSTRACT
The present paper proposes to employ the cultured tumor cells of the breast and chick fibroblasts after long-term cultivation (for above 24 days) to determine their individual drug sensitivity and, as a criterion of cell damage, to use the percent of destruction of the cell layer formed in the wells 24 hours after drug insertion. It also presents the comparative results of tests of 2 cellular models that have been used to determine the in vitro sensitivity of the cells of breast cancer and chick fibroblasts to melfalan and its complex compound with copper acetylacetonate - MOK*M. At the same time, the cytotoxic activity of MOK*M and melfalan against tumor cells has been not shown to differ greatly (16.02+/-1.85 and 15.71+/-0.65% cell layer destruction, respectively), but the same activity of MOK*M against the model of intact cells (chick fibroblasts) was much less (15.23+/-1.97%) than that of melfalan (95.39+/-1.11%). The test system proposed by the authors is of certain informative value and it may be used for the determination of the individual sensitivity of tumor cells to antitumor drugs.
Subject(s)
Antineoplastic Agents/pharmacology , Copper , Melphalan/analogs & derivatives , Melphalan/pharmacology , Organometallic Compounds/pharmacology , Animals , Breast Neoplasms , Cell Survival/drug effects , Cells, Cultured , Chick Embryo , Drug Screening Assays, Antitumor , Female , Fibroblasts/drug effects , Humans , Tumor Cells, CulturedSubject(s)
Air Pollution/adverse effects , Neoplasms/etiology , Air Pollutants/adverse effects , Air Pollutants/analysis , Air Pollution/analysis , Air Pollution/statistics & numerical data , Benzo(a)pyrene/adverse effects , Benzo(a)pyrene/analysis , Humans , Incidence , Morbidity/trends , Neoplasms/epidemiology , Polycyclic Compounds/adverse effects , Polycyclic Compounds/analysis , Urban Population/statistics & numerical data , Uzbekistan/epidemiologySubject(s)
Air Pollutants/analysis , Benzo(a)pyrene/analysis , Power Plants , Coal , Fuel Oils , Smoke/analysis , UzbekistanABSTRACT
Investigations showed that heat plants pollute atmospheric air in towns of Khabarovsk district. Concentrations of benzo(a)pyrene in air of Khabarovsk, Komsomolsk-on-Amur, and Amursk are 4-14 times higher than its maximum allowable concentration.
Subject(s)
Air Pollution/analysis , Benzo(a)pyrene , Carcinogens, Environmental , Environmental Monitoring , Industrial Waste , Polycyclic Compounds , Power Plants , Maximum Allowable ConcentrationABSTRACT
Properties of black bilayer membranes formed from sarcoma-45 phospholipids differed from those of normal cell phospholipids bilayers. Two types of Ca2+-channels were discovered: those multiple to 35 pA and those multiple to 90 pA. The study of bilayers from phospholipids of nuclei, mitochondria, microsomal fractions showed that Ca2+-channels were localized in the microsomal fraction of transformed cells. The relationship between DB18C6 diacetylderivatives membrane activity and their effect to growth of sarcoma-45 in vivo was discovered: the Ca2+-channel blocker diacetyl-DB18C6 inhibited the transformed cells growth.