ABSTRACT
Microelectronic-based sensors are ideal for real-time continuous monitoring of health states due to their low cost of production, small size, portability, and ease of integration into electronic systems. However, typically semiconductor-based devices cannot be operated in aqueous solutions, especially in solutions with a low pH. However, in this work we overcame this difficulty and demonstrated the feasibility of a hemoglobin sensing array based on hybrid organic GaAs-based devices, which can remain in biological solutions for more than 24h. This was achieved by coating devices with a nanometer-thick polymer protective layer with subsequent adsorption of antibodies on its surface. The device is capable of functioning even in harsh physiological fluids, such as urine and bile juice. The surface modification allows a change in electrical potential, created by the interaction, to be efficiently transferred to the surface of the semiconductor device. By utilizing an array configuration, it is possible to obtain high sensitivity and selectivity.
Subject(s)
Biosensing Techniques , Hemoglobins/isolation & purification , Quantum Dots , Antibodies/immunology , Antibodies/isolation & purification , Arsenicals/chemistry , Gallium/chemistry , Hemoglobins/immunology , Hemoglobins/metabolism , Humans , Hydrogen-Ion Concentration , Organic Chemicals , Surface PropertiesABSTRACT
By applying magnetolithography it is possible to chemically pattern the inside of tubes. This new capability allows one to perform sequential processes within the tubes. Several enzymatic reactions are demonstrated.
