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1.
Nature ; 412(6846): 491-2, 2001 Aug 02.
Article in English | MEDLINE | ID: mdl-11484032
3.
Angew Chem Int Ed Engl ; 40(16): 3071-3, 2001.
Article in English | MEDLINE | ID: mdl-12203653

ABSTRACT

Nanoscale construction work with DNA: Dip-pen nanolithography (DPN) is used to generate nanostructures which can be subsequently functionalized with oligonucleotides a' and b' and used to assemble, in an orthogonal manner, gold nanoparticles (a, b in scheme) functionalized with sequences complementary to the DPN-generated structures.

4.
Science ; 289(5485): 1757-60, 2000 Sep 08.
Article in English | MEDLINE | ID: mdl-10976070

ABSTRACT

A method for analyzing combinatorial DNA arrays using oligonucleotide-modified gold nanoparticle probes and a conventional flatbed scanner is described here. Labeling oligonucleotide targets with nanoparticle rather than fluorophore probes substantially alters the melting profiles of the targets from an array substrate. This difference permits the discrimination of an oligonucleotide sequence from targets with single nucleotide mismatches with a selectivity that is over three times that observed for fluorophore-labeled targets. In addition, when coupled with a signal amplification method based on nanoparticle-promoted reduction of silver(I), the sensitivity of this scanometric array detection system exceeds that of the analogous fluorophore system by two orders of magnitude.


Subject(s)
Oligonucleotide Array Sequence Analysis/methods , Oligonucleotide Probes , Base Pair Mismatch , Base Pairing , Carbocyanines , Fluorescent Dyes , Gold , Microscopy, Confocal , Microscopy, Fluorescence , Nucleic Acid Hybridization , Oligonucleotide Array Sequence Analysis/instrumentation , Sensitivity and Specificity , Temperature
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