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1.
Chem Commun (Camb) ; 49(28): 2879-81, 2013 Apr 11.
Article in English | MEDLINE | ID: mdl-23446278

ABSTRACT

Using the RNA transcript as a template, RNA-templated DNA origami structures were constructed by annealing with designed DNA staple strands. RNA-templated DNA origami structures were folded to form seven-helix bundled rectangular structures and six-helix bundled tubular structures. The chemically modified RNA-DNA hybrid origami structures were prepared by using RNA templates containing modified uracils.


Subject(s)
DNA/chemistry , Nucleic Acid Conformation , RNA/chemistry , Nucleic Acid Hybridization
3.
Dig Dis Sci ; 57(8): 2122-9, 2012 Aug.
Article in English | MEDLINE | ID: mdl-22451121

ABSTRACT

BACKGROUND: One of the problems with endoscopic submucosal dissection (ESD) for early gastric cancer is that it prolongs procedure time considerably. AIM: The purpose of this study was to investigate whether a videoendoscope with water-jet function shortened the time of ESD for early gastric cancer. METHODS: A total of 82 early gastric cancers that were intramucosal, differentiated-type adenocarcinoma ≤2 cm, without ulcer or scar, in 75 consecutive patients were investigated. Three supervised resident endoscopists participated as operators. After stratification by the operator and tumor location, the lesions were randomly assigned to the water-jet videoendoscope or a conventional videoendoscope groups. An insulated tipped knife was used for the ESD procedure. Total operation time was evaluated as a primary endpoint. RESULTS: The median (25-75th percentile) total operation time for the water-jet videoendoscope group was 51 (33-87) minutes, which was shorter than the 62 (43-88) minutes for the conventional videoendoscope, but it did not reach significance (P = 0.201). Multivariate analysis revealed that the water-jet videoendoscope (OR 3.0, P = 0.046), tumor size ≤14 mm (OR 3.2, P = 0.040) and antral tumor (OR 4.5, P = 0.046) were significantly associated with short (≤60 min) operation time. CONCLUSIONS: The water-jet videoendoscope may reduce operation time of ESD for early gastric cancer, compared with conventional videoendoscope. A large-scale multicenter trial is warranted to clarify the efficacy of the water-jet videoendoscope for gastric ESD.


Subject(s)
Adenocarcinoma/surgery , Gastroscopy/instrumentation , Stomach Neoplasms/surgery , Aged , Female , Gastroscopy/adverse effects , Humans , Male , Prospective Studies , Time Factors
4.
J Org Chem ; 76(10): 4052-60, 2011 May 20.
Article in English | MEDLINE | ID: mdl-21480664

ABSTRACT

Linear polystyrene-stabilized PdO nanoparticles (PS-PdONPs) were prepared in water by thermal decomposition of Pd(OAc)(2) in the presence of polystyrene. The immobilization degree of palladium was dependent on the molecular weight of polystyrene, while the size of the Pd nanoparticles was not. Linear polystyrene-stabilized Pd nanoparticles (PS-PdNPs) were also prepared using NaBH(4) and phenylboronic acid as reductants. The catalytic activity of PS-PdONPs was slightly higher than that of PS-PdNPs for Suzuki coupling reaction in water. PS-PdONPs exhibited high catalytic activity for Suzuki and copper-free Sonogashira coupling reactions in water and recycled without loss of activity.

5.
Gan To Kagaku Ryoho ; 37(12): 2340-2, 2010 Nov.
Article in Japanese | MEDLINE | ID: mdl-21224566

ABSTRACT

A man in his early seventies underwent low anterior resection and partial resection of the liver for the rectal cancer and liver metastasis. However, 4 months after the surgery, he was found to have a liver tumor at S5 and S7 by abdominal CT scan. Then, he underwent chemotherapy (mFOLFOX6), but the metastatic tumor was progressive. We selected FOLFIRI + cetuximab regimen for second-line therapy to resect the metastatic tumor. As the metastatic lesion was become smaller after 4-course of the regimen including cetuximab, we decided to perform a radical resection. We conducted a right lobectomy of the liver, and the tumor was completely resected.


Subject(s)
Adenocarcinoma/therapy , Antibodies, Monoclonal/administration & dosage , Antineoplastic Agents/administration & dosage , Antineoplastic Combined Chemotherapy Protocols/administration & dosage , Colonic Neoplasms/pathology , Hepatectomy , Liver Neoplasms/secondary , Liver Neoplasms/therapy , Adenocarcinoma/drug therapy , Adenocarcinoma/pathology , Aged , Antibodies, Monoclonal, Humanized , Camptothecin/administration & dosage , Camptothecin/analogs & derivatives , Cetuximab , Combined Modality Therapy , Fluorouracil/administration & dosage , Humans , Leucovorin/administration & dosage , Male
6.
Gan To Kagaku Ryoho ; 37(12): 2346-8, 2010 Nov.
Article in Japanese | MEDLINE | ID: mdl-21224568

ABSTRACT

A woman in her fifties underwent a right hemicolectomy (D3) for cancer of the ascending colon in October 2007, definitively and pathologically diagnosed as papillary adenocarcinoma invading to the subserosa, and no metastasis was detected to lymph node. But 13 months after the surgery, she was found to have a mass near the anastomosis by an abdominal CT scan. Colonoscopy showed an evaluating lesion with ulcer in the anal side of the anastomosis. We tried to resect the metastasis, but it was not resectable because of the invasion to the pancreas. The mFOLFOX regimen was effective. After the chemotherapy (6 courses), we decided to perform a radical resection. We conducted pancreatoduodenectomy in May 2009. She is still alive 12 months after surgery.


Subject(s)
Adenocarcinoma, Papillary/pathology , Adenocarcinoma, Papillary/therapy , Antineoplastic Combined Chemotherapy Protocols/therapeutic use , Colonic Neoplasms/pathology , Colonic Neoplasms/therapy , Lymphatic Metastasis/pathology , Pancreatic Neoplasms/secondary , Pancreatic Neoplasms/therapy , Duodenum/surgery , Female , Fluorouracil/therapeutic use , Humans , Leucovorin/therapeutic use , Middle Aged , Neoplasm Recurrence, Local , Organoplatinum Compounds/therapeutic use , Pancreatectomy
7.
Gan To Kagaku Ryoho ; 36(3): 519-21, 2009 Mar.
Article in Japanese | MEDLINE | ID: mdl-19295285

ABSTRACT

Our hospital fights against a clinical study clearly. However, our hospital did not participate in a clinical study here. When I widened a clinical study in cooperation with the staff, I make a progress report. I started a clinical study only with a doctor first. Therefore it was recognized many problems happened and to promote a clinical study without cooperation of the work of many kinds. I studied with the staff to promote a clinical study and deepened understanding. Thereafter the clinical study was led by a nurse of the chemotherapy room; , in addition, was able to get various cooperation from a skiagrapher. When OGSG0603 began, the nurse and the pharmacist served to become the key than a doctor. I was able to promote a clinical study by cooperation of much staff. In addition, I was able to carry out the system improvement of each section by performing a clinical study.


Subject(s)
Hospitals , Clinical Trials as Topic , Humans , Patient Care Team
8.
Gastrointest Endosc ; 70(1): 52-9, 2009 Jul.
Article in English | MEDLINE | ID: mdl-19249043

ABSTRACT

BACKGROUND: Strictures of the pancreatic duct may be caused by a variety of underlying pathologic conditions that imaging examinations often fail to define. Conventional procedures for acquisition of a specimen for cytology during ERCP have been limited in their ability to discriminate pancreatic-ductal strictures. OBJECTIVE: Our aim was to discriminate pancreatic-ductal strictures by a new technique of sampling material for cytodiagnosis: scraping cytology with a guidewire. DESIGN: A retrospective study. SETTING: A single cancer center. PATIENTS AND METHODS: Eighty-six patients with pancreatic-ductal strictures composed of 71 malignant and 15 benign diseases were evaluated. Malignant diseases included 70 pancreatic carcinomas and 1 endocrine tumor; benign diseases included the following: 7 chronic pancreatitis, 3 autoimmune pancreatitis, 3 idiopathic pancreatic-ductal strictures, and 2 pancreatic cysts. During ERCP, pancreatic juice was collected with a cannula in the main duct just below the stricture after scraping it with a 0.025-inch hydrophilic guidewire. Cytodiagnosis of the specimen was performed by the Papanicolaou method. MAIN OUTCOME MEASUREMENTS: Diagnostic sensitivities and specificities of scraping cytology with a guidewire for pancreatic carcinoma. RESULTS: Scraping cytology with a guidewire yielded 93% sensitivity, 100% specificity, 100% positive predictive value, 75% negative predictive value, and 94% accuracy. Sensitivities for pancreatic carcinoma in the head, body, and tail of the pancreas were 91%, 100%, and 91%, respectively. Sensitivities for pancreatic carcinoma with a tumor of <20 mm, 21 to 40 mm, 41 to 60 mm, and >61 mm were 95%, 92%, 100%, and 100%, respectively. Pancreatitis subsequent to the procedure occurred in 4 patients (5%), all of whom were cured by conservative treatment. CONCLUSIONS: Benign or malignant pancreatic-ductal strictures were accurately discriminated by scraping cytology with a guidewire during ERCP. The technique yielded high diagnostic sensitivities in pancreatic carcinoma, regardless of the location or size of the tumor.


Subject(s)
Cytological Techniques/methods , Pancreatic Ducts/pathology , Adult , Aged , Aged, 80 and over , Biopsy, Fine-Needle/methods , Cholangiopancreatography, Endoscopic Retrograde , Constriction, Pathologic , Female , Humans , Male , Middle Aged , Prognosis , Reproducibility of Results , Retrospective Studies , Sensitivity and Specificity
9.
Gastric Cancer ; 12(4): 219-24, 2009.
Article in English | MEDLINE | ID: mdl-20047127

ABSTRACT

BACKGROUND: Using a novel autofluorescence imaging video endoscopy system (AFI), tumors in the esophagus and the colon appeared purple in a green background, but the color patterns of early gastric cancer (EGC) were found to vary. Factors associated with these patterns remain unknown. The aims of the present study were to classify the color patterns of EGCs and to investigate the correlation between the patterns and clinicopathological features. METHODS: A total of 107 EGCs that had been evaluated by AFI endoscopy, prior to endoscopic or surgical resection, were included. The color patterns of EGCs in AFI images and the association between tumor color and clinicopathological factors were evaluated. These factors included tumor morphology, location, size, background color, histological type, depth of invasion, lymphatic or vessel permeation, and ulceration. RESULTS: The color patterns of EGCs were classified into the following four groups: purple tumors in a green background (52%); green tumors with a purple margin in a green background (21%); green tumors in a purple background (17%); and purple tumors in a purple background (10%). Univariate analysis showed that macroscopic type, histological type, ulceration, and background AFI color were significantly associated with tumor color, whereas multivariate analysis revealed that macroscopic type was the only independent contributor to tumor color. CONCLUSION: The present study has enabled a clearer understanding of the significance of tumor color in relation to the AFI imaging of EGCs. Recognition of the color patterns in AFI images should help in the diagnosis of EGCs.


Subject(s)
Early Detection of Cancer/methods , Endoscopy, Gastrointestinal/methods , Stomach Neoplasms/diagnosis , Aged , Color , Databases, Factual , Female , Fluorescence , Humans , Male , Middle Aged , Multivariate Analysis , Neoplasm Invasiveness/pathology , Prospective Studies , Stomach Neoplasms/pathology , Video Recording
10.
Gastrointest Endosc ; 69(2): 213-8, 2009 Feb.
Article in English | MEDLINE | ID: mdl-18718584

ABSTRACT

BACKGROUND: Despite its high sensitivity for detecting esophageal cancer, chromoendoscopy with iodine staining has low specificity and requires many biopsy specimens, which is undesirable. Dramatic color change after iodine staining (initially whitish yellow and then pink 2 to 3 minutes later) has come to be recognized as the pink-color sign in cancerous lesions. OBJECTIVE: The aim of the present study was to verify the accuracy of the pink-color sign for diagnosing esophageal squamous neoplasms by a quantitative analysis. DESIGN: A quantitative analysis of endoscopic images was performed by using prospectively pooled data. SETTING: A cancer referral center. PATIENTS: From December 2003 to September 2005, consecutive patients who underwent a procedure by an experienced endoscopist and had iodine-unstained lesions measuring 5 to 20 mm in diameter without obvious protrusions or ulcers were prospectively enrolled. MAIN OUTCOME MEASUREMENTS: The diagnostic ability of the quantitative assessment of the color change at 2 minutes after iodine staining (late phase) was investigated by using a receiver operating characteristic analysis. RESULTS: A total of 45 iodine-unstained lesions in 32 patients were included in the study. Among these, 22 of 23 pink-color-positive lesions were diagnosed as high-grade intraepithelial neoplasias or invasive cancers, whereas 19 of 22 pink-color-negative lesions were diagnosed as nonneoplastic lesions or low-grade intraepithelial neoplasias. The area under the receiver operating characteristic curve was 0.94, indicating excellent validity of the test. A sensitivity of 88% and specificity of 95% were obtained. LIMITATIONS: Single-center retrospective analysis. CONCLUSIONS: We verified the accuracy of the pink-color sign for diagnosing esophageal squamous neoplasms by a quantitative analysis.


Subject(s)
Carcinoma, Squamous Cell/pathology , Esophageal Neoplasms/pathology , Iodine , Aged , Color , Esophagoscopy , Female , Humans , Male , Middle Aged , Oropharyngeal Neoplasms/pathology
12.
Gastrointest Endosc ; 67(6): 799-804, 2008 May.
Article in English | MEDLINE | ID: mdl-18158151

ABSTRACT

BACKGROUND: A new technique, endoscopic submucosal dissection (ESD), which uses specially developed endoscopic knives, was recently developed for en bloc resection of large lesions. Despite increasing indications for endoscopic resection (ER), there are limited data available regarding the outcome of ER for lesions 20 mm or more in diameter. OBJECTIVE: To investigate the risk factors for local recurrence. DESIGN: Retrospective cohort study. SETTING: A cancer-referral center. PATIENTS: Seventy patients, who presented between September 1994 and April 2006, with a total of 78 lesions that measured 20 mm or more in diameter. MAIN OUTCOME MEASUREMENT: Local recurrence rate after ER was assessed. RESULTS: At a median follow-up of 32 months (range 12-121 months), there were 12 local recurrences (15.4%). There was no significant association between local recurrence and multiple iodine-voiding lesions, tumor size, or tumor location. The number of resections and the resection method, however, were significantly associated with local recurrence. There was no recurrence of lesions treated by en bloc resection. Lesions resected in 5 or more pieces had a significantly higher recurrence rate than lesions resected in 2 to 4 pieces. Lesions treated by EMR had a significantly higher recurrence rate than lesions treated by ESD. LIMITATIONS: Single-center retrospective analysis. CONCLUSIONS: Esophageal squamous-cell carcinoma that measured 20 mm or more in diameter should be resected en bloc by ESD. Lesions treated by resection in 5 or more pieces have a higher risk for local recurrence.


Subject(s)
Carcinoma, Squamous Cell/surgery , Endoscopy, Gastrointestinal/adverse effects , Esophageal Neoplasms/surgery , Esophagectomy/adverse effects , Neoplasm Recurrence, Local/etiology , Aged , Aged, 80 and over , Carcinoma, Squamous Cell/pathology , Endoscopy, Gastrointestinal/methods , Esophageal Neoplasms/pathology , Esophagectomy/methods , Female , Follow-Up Studies , Humans , Male , Middle Aged , Neoplasm Recurrence, Local/pathology , Prognosis , Retrospective Studies , Risk Factors
13.
Am J Gastroenterol ; 102(8): 1610-6, 2007 Aug.
Article in English | MEDLINE | ID: mdl-17403076

ABSTRACT

OBJECTIVES: With conventional methods of endoscopic mucosal resection for early gastric cancer (EGC), proton pump inhibitors (PPIs) and H2-receptor antagonists (H2RAs) have a similar effect on preventing bleeding from artificial ulcers. An objective of this study is to investigate whether a stronger acid suppressant (i.e., PPI) more effectively prevents bleeding after the recent advanced technique of endoscopic submucosal dissection (ESD) for EGC. METHODS: This was a prospective randomized controlled trial performed in a referral cancer center. A total of 143 patients with EGC who underwent ESD were randomly assigned to the treatment groups. They received either rabeprazole 20 mg (PPI group) or cimetidine 800 mg (H2RA group) on the day before ESD and continued for 8 wk. The primary end point was the incidence of bleeding that was defined as hematemesis or melena that required endoscopic hemostasis and decreased the hemoglobin count by more than 2 g/dL. RESULTS: In baseline data, the endoscopists who performed the ESD were significantly different between the groups. Finally, 66 of 73 patients in the PPI group and 64 of 70 in the H2RA group were analyzed. Bleeding occurred in four patients in the PPI group and 11 in the H2RA group (P= 0.057). Multivariate analysis revealed that treatment with the PPI significantly reduced the risk of bleeding: adjusted hazard ratio 0.47, 95% confidence interval 0.22-0.92, P= 0.028. One delayed perforation was experienced in the H2RA group. CONCLUSIONS: PPI therapy more effectively prevented delayed bleeding from the ulcer created after ESD than did H2RA treatment.


Subject(s)
2-Pyridinylmethylsulfinylbenzimidazoles/therapeutic use , Anti-Ulcer Agents/therapeutic use , Cimetidine/therapeutic use , Histamine H2 Antagonists/therapeutic use , Peptic Ulcer Hemorrhage/prevention & control , Proton Pump Inhibitors , Stomach Neoplasms/surgery , Adult , Aged , Aged, 80 and over , Female , Gastroscopy , Humans , Male , Middle Aged , Multivariate Analysis , Peptic Ulcer Hemorrhage/etiology , Postoperative Complications , Prospective Studies , Rabeprazole
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