ABSTRACT
A rapid procedure is described for detecting and differentiating pseudomonads by their ability to degrade substrates with diverse physico-chemical properties, including volatile and non-volatile water-insoluble compounds, strong organic acids and bases, surfactants, disinfectants, and other toxic substances. The bacteria are embedded in an agar medium containing mineral salts and exposed to about 5 mg amounts of six to eight different substrates placed on the surface of an agar plate. After incubation at 25 degrees C for 24 hrs, growth zones around utilizable substrates may be used to differentiate various Pseudomonas strains and species.
Subject(s)
Pseudomonas/classification , Bacteriological Techniques , Culture Media , Pseudomonas/metabolism , Pseudomonas aeruginosa/classification , Pseudomonas aeruginosa/metabolism , Pseudomonas fluorescens/classification , Pseudomonas fluorescens/metabolismABSTRACT
The constitutive NADP+-dependent alcohol dehydrogenase from Acinetobacter calcoaceticus can be accumulated about 50 fold in 3 purification steps. The end-product shows in the analytical polyacrylamide gel electrophoresis only one active enzyme band. The molecular weight of the enzyme was determined to be 235,000 by gel chromatography on Sephadex G 200, the smallest subunit shows a molecular weight of 61 000 on SDS electrophoresis. The isoelectric point is at 5.84. The KM values determined with primary aliphatic alcohols diminish in the range of the homologous order (C2--C10) with growing chain length. The KM value for hexanal is about 20 fold less than that for 1-hexanol.
