1.
Biochem Int
; 25(3): 537-45, 1991 Oct.
Article
in English
| MEDLINE
| ID: mdl-1805798
ABSTRACT
125I-insulin (10 fmoles) binding plus internalization (BI) to a clonal capillary endothelial (CE) cell line reached to a steady state after 20 min. Acid-washed fraction accounted for nearly half of the total specifically-bound hormone. Dissociation constants (Kd) for insulin-surface receptor in acid-extractable fraction were 0.04 nM (high affinity) and 4.7 nM (low affinity) with a total number of 210,000 high affinity receptors per cell. When 125I-labeled IGF-1 (15 fmoles) was incubated similarly, BI reached only a quasi-equilibrium by 6 min and continued to increase thereafter. 2-Deoxyglucose transport in these cells was stimulated by insulin whereas IGF-1 inhibited its entry.