[[Gender Differences in Tissue Cascade of Activation of Plasminogen and PAI-1 in Esophageal Squamous Cell Carcinoma].]

AIM: comparative analysis of uPA-Ag, uPA-act, tPA-Ag, tPA-act, PAl--Ag and PAl-i-act in tissues of esophageal squamous cell carcinoma (ESCC) in men (M) and women (W) to clarify some pathogenesis issues. MATERIAL AND METHODS: Tumor tissue of ESCC and its perifocal area (19 M and 8 menopausal W aged 38-72 years, st 1I, G2, T,3N M0) were studied by ELISA using standard test kits. RESULTS: ESCC tissue of M showed an increase in both uPA types from the resection line (RL), while in W only uPA-act was increased. tPA-Ag was decreased in tumors of W, tPA-act - in tumors of all patients. Levels of both PAl-- types were higher in ESCC of M than in W. in M, tPA-Ag in perifocal area was lower than in RL, while in W, on the contrary, it was higher. tPA- Ag/tPA-act coefficient in M tumors was 5.7 times higher than in W; in perifocal area it was reduced in all patients, being at the same time in M lower than in W. Both PAl- types were increased in malignant tissues of all patients, prevailing in M tumors, and PAl-i-Ag in peritumoral tissue was higher in M than in W. CONCLUSIONS: Significant gender differences were found in expression of uPA, tPA and PAl-i in tissues of esophageal squamous cell carcinoma. Presence of tumor-associated uPA, PAH and tPA in men and uPA and PAl-i in women is possible in tumor tissue of esophageal squamous cell carcinoma and its perifocal area. Determination of plasminogen regulation system in tissues of esophageal squamous cell carcinoma can be used for the selection and individualization of postoperative treatments.

[Changes in markers of proliferation, neoangiogenesis and plasminogen activation system in rectal cancer tissue].

AIM OF THE STUDY: determination of levelsofsometissuegrowth factors, fibrinolyticsystemindicesand MMP-3 for specifi- cation of the role of their changes in moderately differentiated adenocarcinoma of the rectum. MATERIALS AND METHODS. Levels of growth factors and neoangiogenesis, tissue fibrinolyticsystemindicesand MMP-3were studiedin cytosols of tissue obtained from 73 patients (primary adenocarcinomas, st. III, G2, 47 men and 26 women aged 38-74 years, T1-3N0M0) by the ELISA method using standard test kits. RESULTS: Increase in the content of growth factors VEGF-A, IGF and TGF-ß1, the receptor protein VEGF-R, plasminogen activator uPA and metalloproteinases MMP-3 was detected inadenocarcinoma of the rectum. Gender differences inVEGF-Aand TGF-ß1 content were found. Increase in levels and activity of uPA and MMP-3 only was detected in perifocal zone of the tumor. Changes in EGF content were found neither in adenocarcinoma tissue nor in its perifocal zone. No significant gender differenceswere observed intissue fibrinolyticsystemandMMP-3. Age differenceswere not found either. CONCLUSION. I. Concurrent expressionof IGF-I, IGF-II, TGF-ß1 and VEGF-Aanditsreceptorinmalignanttumortissue, as well as increasedplasmin release from proenzyme and MMP-3 activationis apparently associatedwith the formation ofpathogenic mechanism of vasculature development in moderately differentiated adenocarcinoma of the rectum. 2. Activation of uPA and MMP-3 in perifocal zone of the tumor can serve as an indexof its invasive activity. 3. Genderdifferencesin VEGF-A and TGF-ß1, content in tumor tissue were observed; significant association between natient gender and levels of IGF, EGF. fibinolvsis indices and MMP-3 was not found.