1.
Chembiochem
; 14(4): 421-5, 2013 Mar 04.
Article
in English
| MEDLINE
| ID: mdl-23371788
ABSTRACT
PHOTO OPPORTUNITY: We have developed a dual photoaffinity labeling system in which an active and an inactive probe bearing orthogonal detection groups are co-reacted in a single photoreaction. The approach allowed selective fluorescent detection of a model binding protein in cell lysate by either 1D or 2D electrophoresis.
Subject(s)
Carbonic Anhydrase II/analysis , Fluorescent Dyes/analysis , Photoaffinity Labels/analysis , Small Molecule Libraries/analysis , Binding Sites , Carbonic Anhydrase II/metabolism , Click Chemistry , Electrophoresis, Gel, Two-Dimensional , Electrophoresis, Polyacrylamide Gel , Fluorescence , Fluorescent Dyes/metabolism , HeLa Cells , Humans , Photoaffinity Labels/metabolism , Protein Binding , Small Molecule Libraries/metabolism
2.
Chem Asian J
; 7(7): 1567-71, 2012 Jun.
Article
in English
| MEDLINE
| ID: mdl-22514195
ABSTRACT
Two are better than one: A new approach to selective photoaffinity labeling is described in which a bioactive probe is used in combination with its inactive analog as a scavenger of nonspecific proteins.
