Harnessing the power of hydrogen sulphide (H2 S) for improving fruit quality traits.

Hydrogen sulphide (H2 S) is a gaseous molecule and originates endogenously in plants. It is considered a potential signalling agent in various physiological processes of plants. Numerous reports have examined the role of H2 S in fruit ripening and in enhancing fruit quality traits. H2 S coordinates the fruit antioxidant system, fruit ripening phytohormones, such as ethylene and abscisic acid, together with other ripening-related signalling molecules, including nitric oxide and hydrogen peroxide. Although many studies have increased understanding of various aspects of this complex network, there is a gap in understanding crosstalk of H2 S with key players of fruit ripening, postharvest senescence and fruit metabolism. This review focused on deciphering fruit H2 S metabolism, signalling and its interaction with other ripening-related signalling molecules during fruit ripening and postharvest storage. Moreover, we also discuss how H2 S can be used as a tool for improving fruit quality and productivity and reducing postharvest loss of perishable fruits.

Experimental colonic anastomosis in bovines--and angiographic study.

4 different methods of colonic anastomosis, single and double layer inversion and eversion, were performed on cowcalves. Hypervascularization of neovascularized arterioles at the cut ends of colon were visualized on the 7th day by all techniques. The arterioles, later one, tended to reorganise towards normalcy. Inversion technique showed better vascularization at the anastomotic site, and the vascular pattern was nearly normal on the 14th and 28th days from surgery. Eversion technique did not exhibit satisfactory revascularization of the anastomotic site on the 7th day of study. A radiopaque area, davoid of arterioles, was noted on the 14th and 28th days from surgery.

Colonic anastomosis in calves: an experimental study.

Colonic anastomosis was conducted in 12 calves divided equally into four groups. Four suture patterns, viz. single inversion (continuous Connell), double inversion (two rows of continuous Cushing), single eversion (continuous everted mattress), double eversion (single eversion reinforced with simple continuous) were used. In each animal, anastomosis of the same technique was performed at four places. Each technique was evaluated in terms of clinical and gross observations, lumen stenosis and bursting pressure. Observations were made on the 7th, 14th and 28th day after anastomosis. None of the anastomotic techniques showed any untoward incident during the 28 days of postoperative study. Stricture formation at the site of anastomosis was minimal with the single inversion technique whereas it was maximum with the single eversion technique. Pressure for disruption of intestinal segments was higher after inversion than eversion techniques. It was concluded that the one-layer inversion anastomosis technique proved to be the best among the four techniques because (i) of its reduced incidence of adhesions; (ii) it maintains adequate lumen diameter; (iii) it withstands maximum pressure. The double layer inversion technique was conceded to be the second best choice for colonic anastomosis followed by the double eversion and single eversion techniques.