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1.
PLoS Negl Trop Dis ; 10(6): e0004787, 2016 06.
Article in English | MEDLINE | ID: mdl-27332553

ABSTRACT

Neurocysticercosis (NCC) is one of the most common helminth parasitic diseases of the central nervous system (CNS) and the leading cause of acquired epilepsy worldwide. NCC is caused by the presence of the metacestode larvae of the tapeworm Taenia solium within brain tissues. NCC patients exhibit a long asymptomatic phase followed by a phase of symptoms including increased intra-cranial pressure and seizures. While the asymptomatic phase is attributed to the immunosuppressive capabilities of viable T. solium parasites, release of antigens by dying organisms induce strong immune responses and associated symptoms. Previous studies in T. solium-infected pigs have shown that the inflammatory response consists of various leukocyte populations including eosinophils, macrophages, and T cells among others. Because the role of eosinophils within the brain has not been investigated during NCC, we examined parasite burden, disease susceptibility and the composition of the inflammatory reaction in the brains of infected wild type (WT) and eosinophil-deficient mice (ΔdblGATA) using a murine model of NCC in which mice were infected intracranially with Mesocestoides corti, a cestode parasite related to T. solium. In WT mice, we observed a time-dependent induction of eosinophil recruitment in infected mice, contrasting with an overall reduced leukocyte infiltration in ΔdblGATA brains. Although, ΔdblGATA mice exhibited an increased parasite burden, reduced tissue damage and less disease susceptibility was observed when compared to infected WT mice. Cellular infiltrates in infected ΔdblGATA mice were comprised of more mast cells, and αß T cells, which correlated with an abundant CD8+ T cell response and reduced CD4+ Th1 and Th2 responses. Thus, our data suggest that enhanced inflammatory response in WT mice appears detrimental and associates with increased disease susceptibility, despite the reduced parasite burden in the CNS. Overall reduced leukocyte infiltration due to absence of eosinophils correlates with attenuated tissue damage and longer survival of ΔdblGATA mice. Therefore, our study suggests that approaches to clear NCC will require strategies to tightly control the host immune response while eradicating the parasite with minimal damage to brain tissue.


Subject(s)
Eosinophilia/genetics , Genetic Predisposition to Disease , Leukocytes/physiology , Neurocysticercosis/pathology , Animals , Female , Mast Cells/physiology , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Neurocysticercosis/genetics , Neutrophils/physiology
2.
PLoS Negl Trop Dis ; 7(3): e2099, 2013.
Article in English | MEDLINE | ID: mdl-23516647

ABSTRACT

In murine neurocysticercosis (NCC), caused by infection with the parasite Mesocestoides corti, the breakdown of the Blood Brain Barrier (BBB) and associated leukocyte infiltration into the CNS is dependent on the anatomical location and type of vascular bed. Prior studies of NCC show that the BBB comprised of pial vessels are most affected in comparison to the BBB associated with the vasculature of other compartments, particularly parenchymal vessels. Herein, we describe a comprehensive study to characterize infection-induced changes in the genome wide gene expression of pial vessels using laser capture microdissection microscopy (LCM) combined with microarray analyses. Of the 380 genes that were found to be affected, 285 were upregulated and 95 were downregulated. Ingenuity Pathway Analysis (IPA) software was then used to assess the biological significance of differentially expressed genes. The most significantly affected networks of genes were "inflammatory response, cell-to-cell signaling and interaction, cellular movement", "cellular movement, hematological system development and function, immune cell trafficking, and "antimicrobial response, cell-to-cell signaling and interaction embryonic development". RT-PCR analyses validated the pattern of gene expression obtained from microarray analysis. In addition, chemokines CCL5 and CCL9 were confirmed at the protein level by immunofluorescence (IF) microscopy. Our data show altered gene expression related to immune and physiological functions and collectively provide insight into changes in BBB disruption and associated leukocyte infiltration during murine NCC.


Subject(s)
Blood Vessels/pathology , Blood-Brain Barrier/pathology , Gene Expression Profiling , Mesocestoides/pathogenicity , Neurocysticercosis/pathology , Animals , Disease Models, Animal , Female , Laser Capture Microdissection , Mice , Mice, Inbred BALB C , Microarray Analysis , Microscopy , Microscopy, Fluorescence , Neurocysticercosis/parasitology , Real-Time Polymerase Chain Reaction
3.
PLoS One ; 8(3): e59616, 2013.
Article in English | MEDLINE | ID: mdl-23527230

ABSTRACT

Sepsis is a complex immune disorder with a mortality rate of 20-50% and currently has no therapeutic interventions. It is thus critical to identify and characterize molecules/factors responsible for its development. We have recently shown that pulmonary infection with Francisella results in sepsis development. As extensive cell death is a prominent feature of sepsis, we hypothesized that host endogenous molecules called alarmins released from dead or dying host cells cause a hyperinflammatory response culminating in sepsis development. In the current study we investigated the role of galectin-3, a mammalian ß-galactoside binding lectin, as an alarmin in sepsis development during F. novicida infection. We observed an upregulated expression and extracellular release of galectin-3 in the lungs of mice undergoing lethal pulmonary infection with virulent strain of F. novicida but not in those infected with a non-lethal, attenuated strain of the bacteria. In comparison with their wild-type C57Bl/6 counterparts, F. novicida infected galectin-3 deficient (galectin-3(-/-)) mice demonstrated significantly reduced leukocyte infiltration, particularly neutrophils in their lungs. They also exhibited a marked decrease in inflammatory cytokines, vascular injury markers, and neutrophil-associated inflammatory mediators. Concomitantly, in-vitro pre-treatment of primary neutrophils and macrophages with recombinant galectin-3 augmented F. novicida-induced activation of these cells. Correlating with the reduced inflammatory response, F. novicida infected galectin-3(-/-) mice exhibited improved lung architecture with reduced cell death and improved survival over wild-type mice, despite similar bacterial burden. Collectively, these findings suggest that galectin-3 functions as an alarmin by augmenting the inflammatory response in sepsis development during pulmonary F. novicida infection.


Subject(s)
Galectin 3/immunology , Immunologic Factors/immunology , Respiratory Tract Infections/complications , Sepsis/etiology , Sepsis/immunology , Tularemia/complications , Animals , Antibodies, Monoclonal , Cytokines/immunology , DNA Primers/genetics , Fluorescent Antibody Technique , Galectin 3/genetics , In Situ Nick-End Labeling , Kaplan-Meier Estimate , Lung/microbiology , Lung/pathology , Mice , Mice, Inbred C57BL , Mice, Knockout , Real-Time Polymerase Chain Reaction , Respiratory Tract Infections/microbiology , Respiratory Tract Infections/physiopathology , Sepsis/metabolism , Tularemia/physiopathology
4.
Infect Immun ; 81(4): 1052-63, 2013 Apr.
Article in English | MEDLINE | ID: mdl-23319563

ABSTRACT

Neurocysticercosis (NCC) is a central nervous system (CNS) infection caused by the metacestode stage of the parasite Taenia solium. During NCC, the parasites release immunodominant glycan antigens in the CNS environment, invoking immune responses. The majority of the associated pathogenesis is attributed to the immune response against the parasites. Glycans from a number of pathogens, including helminths, act as pathogen-associated molecular pattern molecules (PAMPs), which are recognized by pattern recognition receptors (PRRs) known as C-type lectin receptors (CLRs). Using a mouse model of NCC by infection with the related parasite Mesocestoides corti, we have investigated the role of mannose receptor C type 1 (MRC1), a CLR which recognizes high-mannose-containing glycan antigens. Here we show that MRC1(-/-) mice exhibit increased survival times after infection compared with their wild-type (WT) counterparts. The decreased disease severity correlates with reduced levels of expression of markers implicated in NCC pathology, such as interleukin-1ß (IL-1ß), IL-6, CCL5, and matrix metalloproteinase 9 (MMP9), in addition to induction of an important repair marker, fibroblast growth factor 2 (FGF2). Furthermore, the immune cell subsets that infiltrate the brain of MRC1(-/-) mice are dramatically altered and characterized by reduced numbers of T cells and the accumulation of granulocytic cells with an immune phenotype resembling granulocytic myeloid-dependent suppressor cells (gMDSCs). The results suggest that MRC1 plays a critical role in myeloid plasticity, which in turn affects the adaptive immune response and immunopathogenesis during murine NCC.


Subject(s)
Granulocyte Precursor Cells/immunology , Lectins, C-Type/deficiency , Mannose-Binding Lectins/deficiency , Membrane Glycoproteins/deficiency , Mesocestoides/immunology , Neurocysticercosis/immunology , Receptors, Cell Surface/deficiency , Animals , Brain/immunology , Brain/pathology , Cytokines/metabolism , Female , Lectins, C-Type/metabolism , Mannose Receptor , Mannose-Binding Lectins/metabolism , Membrane Glycoproteins/metabolism , Mesocestoides/pathogenicity , Mice , Mice, Inbred C57BL , Neurocysticercosis/mortality , Neurocysticercosis/pathology , Receptors, Cell Surface/metabolism , Receptors, Immunologic , Severity of Illness Index , Survival Analysis
5.
J Egypt Soc Parasitol ; 42(1): 183-90, 2012 Apr.
Article in English | MEDLINE | ID: mdl-22662607

ABSTRACT

Neurocysticercosis is a serious zoonotic diseas, encountered worldwide, caused by the larval stage of Taenia solium. Due to the difficulties facing scientists to study the biological, histological and immunological effects of these larvae on the human brain, other cestodes with more or less similar larvae (Taenia crassiceps) were used. In brain infected mice, Th1 predominant cytokines were significantly detected.


Subject(s)
Cestoda/classification , Cysticercosis/immunology , Cysticercosis/parasitology , Animals , Brain Diseases/immunology , Brain Diseases/parasitology , Brain Diseases/pathology , Humans , Mice , Mice, Inbred BALB C
6.
J Neuroinflammation ; 9: 141, 2012 Jun 25.
Article in English | MEDLINE | ID: mdl-22731103

ABSTRACT

BACKGROUND: Central nervous system (CNS) barriers play a pivotal role in the protection and homeostasis of the CNS by enabling the exchange of metabolites while restricting the entry of xenobiotics, blood cells and blood-borne macromolecules. While the blood-brain barrier and blood-cerebrospinal fluid barrier (CSF) control the interface between the blood and CNS, the ependyma acts as a barrier between the CSF and parenchyma, and regulates hydrocephalic pressure and metabolic toxicity. Neurocysticercosis (NCC) is an infection of the CNS caused by the metacestode (larva) of Taenia solium and a major cause of acquired epilepsy worldwide. The common clinical manifestations of NCC are seizures, hydrocephalus and symptoms due to increased intracranial pressure. The majority of the associated pathogenesis is attributed to the immune response against the parasite. The properties of the CNS barriers, including the ependyma, are affected during infection, resulting in disrupted homeostasis and infiltration of leukocytes, which correlates with the pathology and disease symptoms of NCC patients. RESULTS: In order to characterize the role of the ependymal barrier in the immunopathogenesis of NCC, we isolated ependymal cells using laser capture microdissection from mice infected or mock-infected with the closely related parasite Mesocestoides corti, and analyzed the genes that were differentially expressed using microarray analysis. The expression of 382 genes was altered. Immune response-related genes were verified by real-time RT-PCR. Ingenuity Pathway Analysis (IPA) software was used to analyze the biological significance of the differentially expressed genes, and revealed that genes known to participate in innate immune responses, antigen presentation and leukocyte infiltration were affected along with the genes involved in carbohydrate, lipid and small molecule biochemistry. Further, MHC class II molecules and chemokines, including CCL12, were found to be upregulated at the protein level using immunofluorescence microscopy. This is important, because these molecules are members of the most significant pathways by IPA analyses. CONCLUSION: Thus, our study indicates that ependymal cells actively express immune mediators and likely contribute to the observed immunopathogenesis during infection. Of particular interest is the major upregulation of antigen presentation pathway-related genes and chemokines/cytokines. This could explain how the ependyma is a prominent source of leukocyte infiltration into ventricles through the disrupted ependymal lining by way of pial vessels present in the internal leptomeninges in murine NCC.


Subject(s)
Ependyma/metabolism , Gene Expression Profiling , Gene Expression Regulation/physiology , Neurocysticercosis/pathology , Animals , Central Nervous System/metabolism , Cytokines/genetics , Cytokines/metabolism , Disease Models, Animal , Female , Histocompatibility Antigens Class II/metabolism , Immune System/metabolism , Laser Capture Microdissection , Mice , Mice, Inbred BALB C , Neurocysticercosis/parasitology , Oligonucleotide Array Sequence Analysis
7.
Cell Immunol ; 269(2): 69-73, 2011.
Article in English | MEDLINE | ID: mdl-21497800

ABSTRACT

The bacterial determinants of pulmonary Francisella induced inflammatory responses and their interaction with host components are not clearly defined. In this study, proteomic and immunoblot analyses showed presence of a cytoplasmic protein elongation factor Tu (EF-Tu) in the membrane fractions of virulent Francisella novicida, LVS and SchuS4, but not in an attenuated F. novicida mutant. EF-Tu was immunodominant in mice vaccinated and protected from virulent F. novicida. Moreover, recombinant EF-Tu induced macrophages to produce inflammatory cytokines in a TLR4 dependent manner. This study shows immune stimulatory properties of a cytoplasmic protein EF-Tu expressed on the membrane of virulent Francisella strains.


Subject(s)
Cytokines/immunology , Francisella/immunology , Macrophages/immunology , Peptide Elongation Factor Tu/immunology , Toll-Like Receptor 4/immunology , Animals , Bronchoalveolar Lavage Fluid/immunology , Cell Membrane/metabolism , Cytokines/metabolism , Francisella/metabolism , Francisella tularensis/immunology , Francisella tularensis/metabolism , Immune Sera/immunology , Immunity, Humoral/immunology , Immunodominant Epitopes/immunology , Immunoglobulin A/immunology , Immunoglobulin G/blood , Immunoglobulin G/immunology , Interleukin-6/metabolism , Macrophages/drug effects , Macrophages/metabolism , Mice , Mice, Inbred C57BL , Mice, Knockout , Peptide Elongation Factor Tu/genetics , Peptide Elongation Factor Tu/metabolism , Peptide Elongation Factor Tu/pharmacology , Recombinant Proteins/genetics , Recombinant Proteins/immunology , Recombinant Proteins/pharmacology , Toll-Like Receptor 2/genetics , Toll-Like Receptor 4/genetics , Toll-Like Receptor 4/metabolism , Tumor Necrosis Factor-alpha/metabolism
8.
Infect Immun ; 79(7): 2586-96, 2011 Jul.
Article in English | MEDLINE | ID: mdl-21482681

ABSTRACT

In a murine model for neurocysticercosis (NCC), intracranial inoculation of the helminth parasite Mesocestoides corti induces multiple Toll-like receptors (TLRs), among which TLR2 is upregulated first and to a relatively high extent. Here, we report that TLR2(-/-) mice displayed significantly increased susceptibility to parasite infection accompanied by increased numbers of parasites in the brain parenchyma compared to infection in wild-type (WT) mice. This coincided with an increased display of microglial nodule formations and greater neuropathology than in the WT. Parasite-infected TLR2(-/-) brains exhibited a scarcity of lymphocytic cuffing and displayed reduced numbers of infiltrating leukocytes. Fluorescence-activated cell sorter (FACS) analyses revealed significantly lower numbers of CD11b(+) myeloid cells, γδ T cells, αß T cells, and B cells in the brains of parasite-infected TLR2(-/-) mice. This correlated with significantly reduced levels of inflammatory mediators, including tumor necrosis factor alpha (TNF-α), gamma interferon (IFN-γ), CCL2, CCL3, and interleukin-6 (IL-6) in the central nervous system (CNS) of TLR2(-/-) mice. As TLR2 has been implicated in immune regulation of helminth infections and as alternatively activated macrophages (AAMs) are thought to play a profound regulatory role in such infections, induction of AAMs in infected TLR2(-/-) mice was compared with that in WT mice. Parasite-infected WT brains showed larger numbers of macrophages/microglia (CD11b(+) cells) expressing AAM-associated molecules such as YM1, Fizz1 (found in inflammatory zone-1 antigen), and arginase 1 than TLR2(-/-) brains, consistent with a protective role of AAMs during infection. Importantly, these results demonstrate that TLR2-associated responses modulate the disease severity of murine NCC.


Subject(s)
Brain/immunology , Cestode Infections/immunology , Macrophage Activation , Macrophages/immunology , Mesocestoides , Neurocysticercosis/immunology , Toll-Like Receptor 2/immunology , Animals , Arginase/metabolism , B-Lymphocytes , Brain/parasitology , Brain/pathology , CD11b Antigen/analysis , Cestode Infections/parasitology , Cestode Infections/pathology , Chemotaxis, Leukocyte , Cytokines/metabolism , Disease Models, Animal , Enzyme-Linked Immunosorbent Assay , Female , Flow Cytometry , Fluorescent Antibody Technique , Inflammation , Inflammation Mediators/metabolism , Intercellular Signaling Peptides and Proteins/analysis , Macrophages/metabolism , Mice , Mice, Inbred C57BL , Mice, Knockout , Microglia/immunology , Neurocysticercosis/parasitology , Neurocysticercosis/pathology , T-Lymphocytes , Toll-Like Receptor 2/biosynthesis , Toll-Like Receptor 2/genetics , Toll-Like Receptor 2/metabolism
9.
Microb Pathog ; 51(1-2): 39-47, 2011.
Article in English | MEDLINE | ID: mdl-21440052

ABSTRACT

The virulence mechanisms of Francisella tularensis, the causative agent of severe pneumonia in humans and a CDC category A bioterrorism agent, are not fully defined. As sepsis is the leading cause of mortality associated with respiratory infections, we determined whether, in the absence of any known bacterial toxins, a deregulated host response resulting in sepsis syndrome is associated with lethality of respiratory infection with the virulent human Type A strain SchuS4 of F. tularensis. The C57BL/6 mice infected intranasally with a lethal dose of SchuS4 exhibited high bacterial burden in systemic organs and blood indicative of bacteremia. In correlation, infected mice displayed severe tissue pathology and associated cell death in lungs, liver and spleen. Consistent with our studies with murine model strain Francisella novicida, infection with SchuS4 caused an initial delay in upregulation of inflammatory mediators followed by development of severe sepsis characterized by exaggerated cytokine release, upregulation of cardiovascular injury markers and sepsis mediator alarmins S100A9 and HMGB1. This study shows that pulmonary tularemia caused by the Type A strain of F. tularensis results in a deregulated host response leading to severe sepsis and likely represents the major cause of mortality associated with this virulent pathogen.


Subject(s)
Francisella tularensis/pathogenicity , Lung Diseases/complications , Sepsis/pathology , Tularemia/complications , Animals , Bacteremia/pathology , Cytokines/blood , Humans , Inflammation , Liver/microbiology , Liver/pathology , Lung/microbiology , Lung/pathology , Lung Diseases/immunology , Lung Diseases/microbiology , Lung Diseases/pathology , Mice , Mice, Inbred C57BL , Sepsis/etiology , Sepsis/immunology , Sepsis/microbiology , Spleen/microbiology , Spleen/pathology , Tularemia/immunology , Tularemia/microbiology , Tularemia/pathology , Up-Regulation , Virulence
10.
Immunol Cell Biol ; 89(2): 167-72, 2011 Feb.
Article in English | MEDLINE | ID: mdl-20585334

ABSTRACT

The macrophage is a versatile cell type that can sense and respond to a particular need based on the conditions of the microenvironment. Some studies have recently suggested that pathogens can directly influence the polarization of macrophages. As Francisella infections are characterized by intense necrotic infiltrates in the lung as well as in distal sites of infection, we sought to investigate whether pulmonary Francisella infections could cause the polarization of alternatively activated macrophages (M2/aaMs). Our results indicate that Francisella infections can cause the polarization of M2/aaM in vivo and that macrophages can be polarized toward an M2/aaM phenotype more potently if dead cell debris is used for stimulation in the presence and absence of Francisella infections. Finally, we also demonstrate that efferocytosis is inhibited in macrophages infected with Francisella, thus providing a potential explanation for the lack of clearance and eventual accumulation of dead cell debris associated with this disease.


Subject(s)
Francisella/immunology , Gram-Negative Bacterial Infections/immunology , Gram-Negative Bacterial Infections/microbiology , Macrophage Activation/immunology , Macrophages/immunology , Macrophages/microbiology , Phagocytosis/immunology , Animals , Arginase/biosynthesis , Biomarkers , Lung/immunology , Lung/microbiology , Lung/pathology , Macrophages/enzymology , Mice , Mice, Inbred C57BL , Models, Immunological , Necrosis , Up-Regulation
11.
J Neuroimmunol ; 232(1-2): 26-34, 2011 Mar.
Article in English | MEDLINE | ID: mdl-21051093

ABSTRACT

In this study, using a murine model for neurocysticercosis, macrophage phenotypes and their functions were examined. Mesocestoides corti infection in the central nervous system (CNS) induced expression of markers associated with alternatively activated macrophages (AAMs) and a scarcity of iNOS, a classically activated macrophage marker. The infection in STAT6(-/-) mice resulted in significantly reduced accumulation of AAMs as well as enhanced susceptibility to infection coinciding with increased parasite burden and greater neuropathology. These results demonstrate that macrophages in the helminth infected CNS are largely of AAM phenotypes, particularly as the infection progresses, and that STAT6 dependent responses, possibly involving AAMs, are essential for controlling neurocysticercosis.


Subject(s)
Brain Diseases/immunology , Macrophage Activation/immunology , Macrophages/immunology , Neurocysticercosis/immunology , STAT6 Transcription Factor/immunology , Animals , Brain Diseases/pathology , Disease Models, Animal , Female , Fluorescent Antibody Technique , Mice , Mice, Inbred C57BL , Mice, Knockout , Neurocysticercosis/pathology , Phenotype , Reverse Transcriptase Polymerase Chain Reaction , STAT6 Transcription Factor/deficiency , STAT6 Transcription Factor/genetics
12.
PLoS One ; 5(11): e14088, 2010 Nov 23.
Article in English | MEDLINE | ID: mdl-21124895

ABSTRACT

BACKGROUND: Pneumonia and pulmonary infections are major causes of mortality among the growing elderly population. Age associated attenuations of various immune parameters, involved with both innate and adaptive responses are collectively known as immune senescence. These changes are likely to be involved with differences in host susceptibility to disease between young and aged individuals. METHODOLOGY/PRINCIPAL FINDINGS: The objective of this study was to assess potential age related differences in the pulmonary host response in mice to the Gram-negative respiratory pathogen, Francisella novicida. We intranasally infected mice with F. novicida and compared various immune and pathological parameters of the pulmonary host response in both young and aged mice. CONCLUSIONS/SIGNIFICANCE: We observed that 20% of aged mice were able to survive an intranasal challenge with F. novicida while all of their younger cohorts died consistently within 4 to 6 days post infection. Further experiments revealed that all of the aged mice tested were initially able to control bacterial replication in the lungs as well as at distal sites of replication compared with young mice. In addition, the small cohort of aged survivors did not progress to a severe sepsis syndrome with hypercytokinemia, as did all of the young adult mice. Finally, a lack of widespread cell death in potential aged survivors coupled with a difference in cell types recruited to sites of infection within the lung confirmed an altered host response to Francisella in aged mice.


Subject(s)
Aging/immunology , Francisella/immunology , Gram-Negative Bacterial Infections/immunology , Lung Diseases/immunology , Animals , Apoptosis/immunology , Cell Survival/immunology , Cytokines/blood , Cytokines/immunology , Female , Francisella/physiology , Gram-Negative Bacterial Infections/microbiology , Host-Pathogen Interactions/immunology , Inflammation Mediators/blood , Inflammation Mediators/immunology , Kaplan-Meier Estimate , Lung/immunology , Lung/microbiology , Lung/pathology , Lung Diseases/microbiology , Mice , Mice, Inbred C57BL , Microscopy, Fluorescence
13.
Parasitology ; 137(3): 359-72, 2010 Mar.
Article in English | MEDLINE | ID: mdl-20109250

ABSTRACT

Neurocysticercosis (NCC) is the most common parasitic disease of the central nervous system (CNS) caused by the larval form of the tapeworm Taenia solium. NCC has a long asymptomatic period with little or no inflammation, and the sequential progression to symptomatic NCC depends upon the intense inflammation associated with degeneration of larvae. The mechanisms involved in these progressive events are difficult to study in human patients. Thus it was necessary to develop an experimental model that replicated NCC. In this review, we describe studies of a murine model of NCC in terms of the release/secretion of parasite antigens, immune responses elicited within the CNS environment and subsequent pathogenesis. In particular, the kinetics of leukocyte subsets infiltrating into the brain are discussed in the context of disruption of the CNS barriers at distinct anatomical sites and the mechanisms contributing to these processes. In addition, production of various inflammatory mediators and the mechanisms involved in their induction by the Toll-like receptor signaling pathway are described. Overall, the knowledge gained from the mouse model of NCC has provided new insights for understanding the kinetics of events contributing to different stages of NCC and should aid in the formulation of more effective therapeutic approaches.


Subject(s)
Mesocestoides/physiology , Neurocysticercosis/parasitology , Animals , Disease Models, Animal , Humans , Mice , Neurocysticercosis/immunology , Taenia/physiology
14.
PLoS One ; 4(11): e7920, 2009 Nov 20.
Article in English | MEDLINE | ID: mdl-19936231

ABSTRACT

BACKGROUND: Francisella tularensis is the causative agent of tularemia and is classified as a Category A select agent. Recent studies have implicated TLR2 as a critical element in the host protective response to F. tularensis infection, but questions remain about whether TLR2 signaling dominates the response in all circumstances and with all species of Francisella and whether F. tularensis PAMPs are predominantly recognized by TLR2/TLR1 or TLR2/TLR6. To address these questions, we have explored the role of Toll-like receptors (TLRs) in the host response to infections with F. tularensis Live Vaccine Strain (LVS) and F. tularensis subspecies (subsp.) novicida in vivo. METHODOLOGY/PRINCIPAL FINDINGS: C57BL/6 (B6) control mice and TLR- or MyD88-deficient mice were infected intranasally (i.n.) or intradermally (i.d.) with F. tularensis LVS or with F. tularensis subsp. novicida. B6 mice survived >21 days following infection with LVS by both routes and survival of TLR1(-/-), TLR4(-/-), and TLR6(-/-) mice infected i.n. with LVS was equivalent to controls. Survival of TLR2(-/-) and MyD88(-/-) mice, however, was significantly reduced compared to B6 mice, regardless of the route of infection or the subspecies of F. tularensis. TLR2(-/-) and MyD88(-/-) mice also showed increased bacterial burdens in lungs, liver, and spleen compared to controls following i.n. infection. Primary macrophages from MyD88(-/-) and TLR2(-/-) mice were significantly impaired in the ability to secrete TNF and other pro-inflammatory cytokines upon ex vivo infection with LVS. TNF expression was also impaired in vivo as demonstrated by analysis of bronchoalveolar lavage fluid and by in situ immunofluorescent staining. CONCLUSIONS/SIGNIFICANCE: We conclude from these studies that TLR2 and MyD88, but not TLR4, play critical roles in the innate immune response to F. tularensis infection regardless of the route of infection or the subspecies. Moreover, signaling through TLR2 does not depend exclusively on TLR1 or TLR6 during F. tularensis LVS infection.


Subject(s)
Francisella tularensis/metabolism , Myeloid Differentiation Factor 88/genetics , Toll-Like Receptor 2/genetics , Toll-Like Receptor 4/genetics , Toll-Like Receptors/metabolism , Tularemia/metabolism , Animals , Bronchoalveolar Lavage , Inflammation , Lung/microbiology , Macrophages/metabolism , Mice , Mice, Inbred C57BL , Mice, Transgenic , Myeloid Differentiation Factor 88/metabolism , Proteasome Endopeptidase Complex , Signal Transduction , Toll-Like Receptor 2/metabolism , Toll-Like Receptor 4/metabolism
15.
Infect Immun ; 77(12): 5369-79, 2009 Dec.
Article in English | MEDLINE | ID: mdl-19786565

ABSTRACT

The symptomatic phase of neurocysticercosis (NCC), a parasitic disease of the central nervous system (CNS) in humans, is characterized by inflammatory responses leading to neuropathology and, in some cases, death. In an animal model of NCC in which mice were intracranially inoculated with the parasite Mesocestoides corti, the infection in mice lacking the myeloid differentiation primary response gene 88 (MyD88(-/-)) resulted in decreased disease severity and improved survival compared with that in wild-type (WT) mice. The CNS of MyD88(-/-) mice was more quiescent, with decreased microgliosis and tissue damage. These mice exhibited substantially reduced primary and secondary microglial nodule formations and lacked severe astrogliotic reactions, which were seen in WT mice. Significantly reduced numbers of CD11b(+) myeloid cells, alphabeta T cells, gammadelta T cells, and B cells were present in the brains of MyD88(-/-) mice in comparison with those of WT mice. This decrease in cellular infiltration correlated with a decrease in blood-brain barrier permeability, as measured by reduced fibrinogen extravasation. Comparisons of cytokine expression indicated a significant decrease in the CNS levels of several inflammatory mediators, such as tumor necrosis factor alpha, gamma interferon, CCL2, and interleukin-6, during the course of infection in MyD88(-/-) mice. Collectively, these findings suggest that MyD88 plays a prominent role in the development of the hyperinflammatory response, which in turn contributes to neuropathology and disease severity in NCC.


Subject(s)
Mesocestoides/immunology , Myeloid Differentiation Factor 88/immunology , Neurocysticercosis/immunology , Neurocysticercosis/pathology , Animals , B-Lymphocytes/immunology , Brain/cytology , Brain/parasitology , Brain/pathology , Cytokines/immunology , Female , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Mice, Knockout , Myeloid Cells/immunology , Myeloid Differentiation Factor 88/deficiency , Severity of Illness Index , Survival Analysis , T-Lymphocytes/immunology
16.
Curr Top Microbiol Immunol ; 336: 83-104, 2009.
Article in English | MEDLINE | ID: mdl-19688329

ABSTRACT

Parasite infections in the central nervous system (CNS) are a major cause of morbidity and mortality worldwide, second only to HIV infection. Finding appropriate therapeutic measures to control CNS parasite infections requires an understanding of the tissue-specific host response. CNS parasitic diseases are invariably associated with persistent T-helper 1 (Th1) cytokine-dependent proinflammatory responses. Although type 1 cytokine-dependent proinflammatory responses are essential to control several types of parasite infections, their persistent production contributes to the development of neuropathology with severe consequences. A family of proteins called Toll-like receptors (TLRs) plays a pivotal role in the induction of inflammatory cytokines during infections and tissue injury. Accumulating evidence indicates that in several CNS parasitic infections such as toxoplasmosis and sleeping sickness, host responses mediated through TLRs contribute to parasite clearance and host survival. However, TLR-mediated responses can also contribute to disease severity, as exemplified in cerebral malaria, neurocysticercosis and river blindness. Thus, TLRs influence the immunopathogenesis of CNS parasitic infections by mechanisms that can either benefit the host or further contribute to CNS pathology. This chapter discusses the immunopathogenesis of parasitic infections in the CNS and the role of TLRs in this process.


Subject(s)
Central Nervous System Infections/immunology , Central Nervous System Infections/parasitology , Helminthiasis/immunology , Protozoan Infections/immunology , Toll-Like Receptors/immunology , Animals , Helminthiasis/parasitology , Humans , Protozoan Infections/parasitology
17.
Infect Immun ; 77(10): 4314-26, 2009 Oct.
Article in English | MEDLINE | ID: mdl-19635830

ABSTRACT

Francisella tularensis is the causative agent of zoonotic tularemia, a severe pneumonia in humans, and Francisella novicida causes a similarly severe tularemia in mice upon inhalation. The correlates of protective immunity, as well as the virulence mechanisms of this deadly pathogen, are not well understood. In the present study, we compared the host immune responses of lethally infected and vaccinated mice to highlight the host determinants of protection from this disease. Intranasal infection with an attenuated mutant (Mut) of F. novicida lacking a 58-kDa hypothetical protein protected C57BL/6 mice from a subsequent challenge with the fully virulent wild-type strain U112 via the same route. The protection conferred by Mut vaccination was associated with reduced bacterial burdens in systemic organs, as well as the absence of bacteremia. Also, there was reduced lung pathology and associated cell death in the lungs of vaccinated mice. Both vaccinated and nonvaccinated mice displayed an initial 2-day delay in upregulation of signature inflammatory mediators after challenge. Whereas the nonvaccinated mice developed severe sepsis characterized by hypercytokinemia and T-cell depletion, the vaccinated mice displayed moderated cytokine induction and contained increased numbers of alphabeta T cells. The recall response in vaccinated mice consisted of a characteristic Th1-type response in terms of cytokines, as well as antibody isotypes. Our results show that a regulated Th1 type of cell-mediated and humoral immunity in the absence of severe sepsis is associated with protection from respiratory tularemia, whereas a deregulated host response leading to severe sepsis contributes to mortality.


Subject(s)
Bacterial Vaccines/immunology , Francisella tularensis/immunology , Francisella tularensis/pathogenicity , Sepsis/prevention & control , Tularemia/prevention & control , Animal Structures/microbiology , Animals , Antibodies, Bacterial/blood , Colony Count, Microbial , Cytokines/blood , Lung/pathology , Lymphocyte Count , Mice , Mice, Inbred C57BL , Sepsis/immunology , Survival Analysis , T-Lymphocytes/immunology , Tularemia/immunology , Vaccines, Attenuated/immunology
18.
Am J Pathol ; 175(2): 685-95, 2009 Aug.
Article in English | MEDLINE | ID: mdl-19574432

ABSTRACT

Murine neurocysticercosis is a parasitic infection transmitted through the direct ingestion of Taenia solium eggs, which differentially disrupts the barriers that protect the microenvironment of the central nervous system. Among the host factors that are involved in this response, matrix metalloproteinases (MMPs) have been recently described as important players. Doxycycline is a commonly prescribed antimicrobial drug that acts as an anti-inflammatory agent with broad inhibitory properties against MMPs. In this study, we examined the effects of doxycycline treatment in a murine model of neurocysticercosis. Animals treated with doxycycline exhibited reduced morbidity and mortality throughout the course of infection. Although similar levels of leukocyte infiltration were observed with both treatment regimens, doxycycline appeared to provide improved conditions for host survival, as reduced levels of apoptosis were detected among infiltrates as well as in neurons. As an established MMP blocker, doxycycline reduced the degradation of junctional complex proteins in parenchymal vessels. In addition, doxycycline treatment was associated with an overall reduction in the expression and activity of MMPs, particularly in areas of leukocyte infiltration. These results indicate that a broad-range inhibitor of MMPs promotes host survival and suggest the potential of doxycycline as a therapeutic agent for the control of inflammatory responses associated with neurocysticercosis.


Subject(s)
Apoptosis/drug effects , Brain/drug effects , Doxycycline/therapeutic use , Enzyme Inhibitors/therapeutic use , Matrix Metalloproteinase Inhibitors , Neurocysticercosis/drug therapy , Taenia solium/drug effects , Animals , Brain/pathology , Female , Mice , Mice, Inbred BALB C , Neurocysticercosis/enzymology , Neurocysticercosis/pathology
19.
J Leukoc Biol ; 86(3): 491-504, 2009 Sep.
Article in English | MEDLINE | ID: mdl-19401387

ABSTRACT

The bacterial or host determinants of lethality associated with respiratory Francisella infections are currently unknown. No exo- or endotoxins that contribute to the severity of this disease have been identified. However, a deregulated host immune response upon infection is characterized by an initial 36- to 48-h delay followed by a rapid and excessive inflammatory response prior to death at 72-120 h. Here, we extend these findings by comparing host immune responses between sublethal and lethal respiratory infections of mice with an attenuated transposon mutant (Mut) of F. novicida (F.n.) strain U112 (sublethal) versus the wild-type (WT) strain (lethal). Infection with WT bacteria, but not the Mut, was characterized by sustained bacteremia and systemic dissemination of the pathogen with temporal increases in bacterial burdens in liver and spleen. Severe pathology with large foci of infiltrates associated with extensive tissue damage was evident in WT-infected lungs, and Mut-infected mice displayed much reduced pathology with intact lung architecture. Similar to other experimental models of severe sepsis, WT- but not the Mut-infected mice exhibited a robust increase in numbers of Gr1+ and CD11b+ cells, while displaying a significant depletion of alphabeta T cells. Further, a dramatic up-regulation of multiple cytokines and chemokines was observed only in lethal WT infection. In addition, an earlier and larger increased expression of S100A9, a known mediator of sepsis, was observed in WT-infected mice. Taken together, these results show that a hyperinflammatory host immune response, culminating in severe sepsis, is responsible for the lethal outcome of respiratory tularemia.


Subject(s)
Francisella/immunology , Gram-Negative Bacterial Infections/immunology , Lung Diseases/genetics , Sepsis/genetics , Tularemia/immunology , Animals , Biomarkers/metabolism , CD11b Antigen/metabolism , CD4-Positive T-Lymphocytes/immunology , CD4-Positive T-Lymphocytes/metabolism , CD4-Positive T-Lymphocytes/microbiology , CD8-Positive T-Lymphocytes/immunology , CD8-Positive T-Lymphocytes/metabolism , CD8-Positive T-Lymphocytes/microbiology , Calgranulin B/metabolism , Cytokines/metabolism , Female , Fluorescent Antibody Technique, Direct , Gram-Negative Bacterial Infections/microbiology , Kinetics , Lung/immunology , Lung/microbiology , Mice , Mice, Inbred C57BL , Mice, Mutant Strains , Neutrophil Infiltration , Receptors, Antigen, T-Cell, alpha-beta/immunology , Tularemia/microbiology , Up-Regulation
20.
Cell Immunol ; 257(1-2): 1-4, 2009.
Article in English | MEDLINE | ID: mdl-19356746

ABSTRACT

Respiratory Francisella infections induce a delayed innate immune response followed by a severe sepsis like condition. In this study, mice infected intranasally with Francisella novicida showed a depletion of alphabeta T cells in lungs while exhibiting large accumulations of other leukocytes correlating with disease severity. The depleted T cells were predominantly CD4(+). The alphabeta T cells in infected mice showed significantly higher levels of Annexin V binding than those in mock control mice suggesting increased apoptosis of T cells. These results suggest that lack of transition from an innate to adaptive host response is associated with lethality of respiratory tularemia.


Subject(s)
CD4-Positive T-Lymphocytes/immunology , CD8-Positive T-Lymphocytes/immunology , Francisella/immunology , Lung Diseases/immunology , Receptors, Antigen, T-Cell, alpha-beta/immunology , Tularemia/immunology , Animals , Annexin A5/immunology , Annexin A5/metabolism , Apoptosis/immunology , CD4-Positive T-Lymphocytes/microbiology , CD8-Positive T-Lymphocytes/microbiology , Female , Lung/immunology , Lung/microbiology , Lung Diseases/microbiology , Lymphocyte Depletion , Mice , Mice, Inbred C57BL , Receptors, Antigen, T-Cell, alpha-beta/metabolism , Tularemia/microbiology
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