Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 26
Filter
Add more filters










Publication year range
1.
Biosci Biotechnol Biochem ; 87(8): 833-838, 2023 Jul 24.
Article in English | MEDLINE | ID: mdl-37169915

ABSTRACT

The development of repellents as alternatives to insecticides has expanded in recent years. However, their use in isopod pest control is limited. To develop an isopod repellent, a plant extract library from wild plants native to the Kochi Prefecture was screened for repellent activity against pillbugs, and 82 samples (87%) exhibited repellent activity. Among them, (E)-7-phenyl-2-heptene-4,6-diyn-1-ol was isolated and identified as a repellent from the root of Bidens pilosa. It had a half-maximal effective concentration of 0.20 µm, with a strong repellency. A study of the structure-activity relationship to (E)-7-phenyl-2-heptene-4,6-diyn-1-ol revealed that the presence of a hydroxyl group and an aromatic at both ends of the length of the seven-carbon chain is important for the expression of repellency. These results can potentially lead to a new repellent of phenylalkyl alcohol.


Subject(s)
Bidens , Isopoda , Animals , Plant Extracts/pharmacology
2.
Phytochemistry ; 184: 112650, 2021 Apr.
Article in English | MEDLINE | ID: mdl-33529859

ABSTRACT

Changes in specialized metabolites were analyzed in barley (Hordeum vulgare) leaves treated with CuCl2 solution as an elicitor. LC-MS analysis of the CuCl2-treated leaves showed the induced accumulation of three compounds. Among them, two were purified by silica gel and ODS column chromatography and preparative HPLC and were identified as 2',3,4,4',6'-pentamethoxychalcone and 2'-hydroxy-3,4,4',6'-tetramethoxychalcone by spectroscopic analyses. The remaining compound was determined as 12-oxo-phytodienoic acid (OPDA), a major oxylipin in plants, by comparing its spectrum and retention time from LC-MS/MS analysis with those of the authentic compound. The accumulation of these compounds was reproduced in leaves inoculated with Bipolaris sorokiniana, the causal agent of spot blotch of the Poaceae species. This inoculation increased the amounts of other oxylipins, including jasmonic acid (JA), JA-Ile, 9-oxooctadeca-10,12-dienoic acid (9-KODE), and 13-oxooctadeca-9,11-dienoic acid (13-KODE). The treatments of the barley leaves with JA and OPDA induced the accumulation of methoxylchalcones, but treatment with 9-KODE did not. These methoxylchalcones inhibited conidial germination of B. sorokiniana and Fusarium graminearum, thereby indicating that these compounds possessed antifungal activity. Consequently, they are considered to be involved in the chemical defense processes as phytoalexins in barley. Accumulation of methoxylchalcones in response to JA treatment was observed in all seven barley cultivars tested, but was not detected in other wild Hordeum species, wheat, and rice, thus indicating that their production was specific to cultivated barley.


Subject(s)
Hordeum , Chromatography, Liquid , Cyclopentanes , Fusarium , Oxylipins/pharmacology , Plant Leaves , Tandem Mass Spectrometry
3.
Biosci Biotechnol Biochem ; 85(4): 743-755, 2021 Mar 24.
Article in English | MEDLINE | ID: mdl-33580659

ABSTRACT

Plant defense responses are activated by various exogenous stimuli. We found that an aqueous extract of spent mushroom substrate used for the cultivation of Hypsizygus marmoreus induced defense responses in rice. Fractionation of the spent mushroom substrate extract indicated that the compounds responsible for this induction were neutral and hydrophilic molecules with molecular weights lower than 3 kDa. Compounds with these characteristics, namely glucose, fructose, and sucrose, were detected in the extract at concentrations of 17.4, 3.3, and 1.6 mM, respectively, and the treatment of rice leaves with these sugars induced defense responses. Furthermore, microarray analysis indicated that the genes involved in defense responses were commonly activated by the treatment of leaves with spent mushroom substrate extract and glucose. These findings indicate that the induction of defense responses by treatment with spent mushroom substrate extract is, at least in part, attributable to the sugar constituents of the extract.


Subject(s)
Agaricales/drug effects , Oryza/physiology , Sugars/pharmacology , Agaricales/chemistry , Genes, Plant , Molecular Weight , Oryza/genetics , Water/chemistry
4.
J Pestic Sci ; 44(2): 89-96, 2019 May 20.
Article in English | MEDLINE | ID: mdl-31148936

ABSTRACT

We investigated the effect of treatment with hot water extracts from the spent mushroom substrates (SMSs) of Lentinula edodes and Hypsizygus marmoreus on the resistance of rice leaves to Pyricularia oryzae infection. The spraying of the SMS extracts clearly suppressed the development of lesions caused by Py. oryzae infection. The accumulation of phytoalexins momilactones A and B, oryzalexin A, and sakuranetin was markedly induced by the spraying of extracts. The enhanced expression of defense related genes PR1b and PBZ was also found in leaves sprayed with the extracts. Treatments with the extracts also affected phytohormone levels. The levels of N 6-(Δ2-isopentenyl)adenine and trans-zeatin markedly increased in response to treatment, whereas the levels of salicylic and jasmonic acids were largely unchanged.

5.
J Pestic Sci ; 43(3): 191-197, 2018 Aug 20.
Article in English | MEDLINE | ID: mdl-30363135

ABSTRACT

The inducible metabolites in rice leaves treated with 1 mM jasmonic acid (JA) were analyzed using HPLC. We detected an increase in the levels of two compounds, 1 and 2. Based on the comparison with mass spectra and chromatographic behavior with authentic compounds, 1 and 2 were identified as 13-oxooctadeca-9,11-dienoic acid (13-KODE) and 9-oxooctadeca-10,12-dienoic acid (9-KODE), respectively, which have not been detected in rice to date. The accumulation of these compounds was also induced by an infection by Bipolaris oryzae. Treatment of rice leaves with KODEs induced the accumulation of defensive secondary metabolites, sakuranetin, naringenin, and serotonin, suggesting that KODEs may play a role in the elicitation of defense responses. The compounds that have an α, ß-unsaturated carbonyl group similar to KODEs did not reproduce the response of accumulation of defensive secondary metabolites, suggesting that additional structural factors such as long hydrophobic carbon chain are needed to elicit defense responses.

6.
Biosci Biotechnol Biochem ; 81(6): 1090-1098, 2017 Jun.
Article in English | MEDLINE | ID: mdl-28485206

ABSTRACT

The inducible metabolites were analyzed in barley leaves inoculated with Bipolaris sorokiniana, the causal agent of spot blotch of barley. HPLC analysis revealed that B. sorokiniana-infected leaves accumulated 4 hydrophilic compounds. They were purified by ODS column chromatography and preparative HPLC. Spectroscopic analyses revealed that they were tyramine (1), 3-(2-aminoethyl)-3-hydroxyindolin-2-one (2), serotonin (3), and 5,5'-dihydroxy-2,4'-bitryptamine (4). Among these, 2 and 4 have not been reported as natural products. They showed antifungal activity in an assay of inhibition of B. sorokiniana conidia germination, suggesting that they play a role in the chemical defense of barley as phytoalexins. The accumulation of 1-4 was examined also in the leaves of rice and foxtail millet. Rice leaves accumulated 2, 3, and 4, whereas foxtail millet leaves accumulated 3 and 4 in response to pathogen attack, suggesting the generality of accumulation of 3 and 4 in the Poaceae species.


Subject(s)
Antifungal Agents/immunology , Hordeum/immunology , Plant Diseases/immunology , Saccharomycetales/drug effects , Sesquiterpenes/immunology , Spores, Fungal/drug effects , Antifungal Agents/metabolism , Antifungal Agents/pharmacology , Chromatography, High Pressure Liquid , Hordeum/metabolism , Hordeum/microbiology , Hydrophobic and Hydrophilic Interactions , Indoles/immunology , Indoles/metabolism , Indoles/pharmacology , Oryza/immunology , Oryza/metabolism , Oryza/microbiology , Plant Diseases/microbiology , Plant Immunity , Plant Leaves/immunology , Plant Leaves/metabolism , Plant Leaves/microbiology , Saccharomycetales/pathogenicity , Saccharomycetales/physiology , Serotonin/biosynthesis , Serotonin/immunology , Serotonin/pharmacology , Sesquiterpenes/metabolism , Sesquiterpenes/pharmacology , Setaria Plant/immunology , Setaria Plant/metabolism , Setaria Plant/microbiology , Species Specificity , Spores, Fungal/pathogenicity , Spores, Fungal/physiology , Tryptamines/biosynthesis , Tryptamines/immunology , Tryptamines/pharmacology , Tyramine/biosynthesis , Tyramine/immunology , Tyramine/pharmacology , Phytoalexins
7.
Biosci Biotechnol Biochem ; 81(3): 431-440, 2017 Mar.
Article in English | MEDLINE | ID: mdl-27854190

ABSTRACT

The Poaceae is a large taxonomic group consisting of approximately 12,000 species and is classified into 12 subfamilies. Gramine and benzoxazinones (Bxs), which are biosynthesized from the tryptophan pathway, are well-known defensive secondary metabolites in the Poaceae. We analyzed the presence or absence of garamine and Bxs in 64 species in the Poaceae by LC-MS/MS. We found that Hordeum brachyantherum and Hakonechloa macra accumulated gramine, but the presence of gramine was limited to small groups of species. We also detected Bxs in four species in the Pooideae and six species in the Panicoideae. In particular, four species in the Paniceae tribe in Panicoideae accumulaed Bxs, indicating that this tribe is a center of the Bx distribution. Bxs were absent in the subfamilies other than Pooideae and Panicoideae. These findings provide an overview of biased distribution of gramine and Bxs in Poaceae species.


Subject(s)
Alkaloids/metabolism , Benzoxazines/metabolism , Poaceae/metabolism , Tryptophan/metabolism , Alkaloids/analysis , Benzoxazines/analysis , Chromatography, Liquid/methods , Indole Alkaloids , Metabolic Networks and Pathways , Secondary Metabolism , Tandem Mass Spectrometry/methods , ortho-Aminobenzoates/analysis , ortho-Aminobenzoates/metabolism
8.
Biosci Biotechnol Biochem ; 80(12): 2285-2290, 2016 Dec.
Article in English | MEDLINE | ID: mdl-27685157

ABSTRACT

To elucidate the probing stimulants in rice plants for the white-backed planthopper, Sogatella furcifera, bioassay-guided separations were conducted, which led to the isolation of four active compounds. Using NMR and LC-MS spectra, their structures were determined as isoorientin 2″-O-(6‴-(E)-feruloyl)glucoside, isoorientin 2″-O-(6‴-(E)-p-coumaroyl)glucoside, tricin 5-O-glucoside, and isoscoparin 2″-O-(6‴-(E)-feruloyl)glucoside.


Subject(s)
Biological Assay , Hemiptera/drug effects , Oryza/chemistry , Animals , Glucosides/chemistry , Glucosides/pharmacology
9.
Z Naturforsch C J Biosci ; 70(5-6): 145-50, 2015.
Article in English | MEDLINE | ID: mdl-26181048

ABSTRACT

The feeding response of larvae of the swallowtail butterfly, Graphium sarpedon nipponum (Lepidoptera: Papilionidae), is elicited by a methanolic extract from camphor tree (Cinnamomum camphora) leaves. Based on bioassay-guided fractionation, three compounds, isolated from the methanolic extract of fresh leaves of the camphor tree, were revealed to be involved in a multi-component system of feeding stimulants. Structures of these feeding stimulants were identified as sucrose, 5-O-caffeoylquinic acid and quercetin 3-O-ß-glucopyranoside by NMR and LC-MS.


Subject(s)
Appetite Stimulants/pharmacology , Cinnamomum camphora/chemistry , Feeding Behavior/drug effects , Larva/drug effects , Lepidoptera/physiology , Plant Extracts/pharmacology , Animals , Chromatography, High Pressure Liquid , Larva/physiology , Lepidoptera/growth & development , Magnetic Resonance Spectroscopy , Spectrophotometry, Ultraviolet
10.
ScientificWorldJournal ; 2012: 214907, 2012.
Article in English | MEDLINE | ID: mdl-23049472

ABSTRACT

The objective of this study was to establish a high quality progeny production system for the house fly parasitoid, Spalangia endius (Hymenoptera: Pteromalidae), by stockpiling hosts. We performed two host killing methods before host storage: (i) heat-killed by 30 min exposure to 50°C or (ii) freeze-killed by 10 min exposure to -80°C. The average number of parasitoids that emerged from nonstored house fly pupae after heat- or freeze-killing was not significantly different from live pupae. When house fly pupae stored at -20°C after heat-killing were supplied to S. endius, progeny production was significantly less than live pupae. Moreover, productivity became very low when house fly pupae refrigerated at 3°C after heat- or freeze-killing were supplied to S. endius. On the other hand, when house fly pupae stored at -80°C for 1 year after heat-killing were supplied to S. endius, the average number of parasitoids that emerged was not significantly different from live pupae. The average number of parasitoids that emerged from freeze-killed hosts kept for more than 8 weeks at -80°C was significantly fewer than live pupae. Thus, this study clarified that a higher-quality host can be maintained not only by simply storing at -80°C but also by adding heat treatment before storage.


Subject(s)
Freezing , Hot Temperature , Houseflies/parasitology , Hymenoptera/pathogenicity , Animals , Biological Control Agents , Houseflies/growth & development , Hymenoptera/growth & development , Pest Control, Biological/methods , Pupa/parasitology , Time Factors
11.
Z Naturforsch C J Biosci ; 65(9-10): 571-6, 2010.
Article in English | MEDLINE | ID: mdl-21138058

ABSTRACT

The acceptance of camphor tree (Cinnamomum camphora) as a host plant for the larvae of common bluebottle (Graphium sarpedon nipponum) was explained by the presence of feeding stimulants in the leaves. When the active methanol extract of C. camphora leaves was separated into hexane and water layers, both layers showed high feeding activities for the larvae of G. sarpedon nipponum. Bioassay-guided fractionation of the hexane layer resulted in the isolation of a highly active compound, which was identified as a-linolenic acid by nuclear magnetic resonance spectrometry and gas chromatography-mass spectrometry.


Subject(s)
Appetite Stimulants/pharmacology , Appetite/physiology , Cinnamomum camphora/chemistry , Lepidoptera/drug effects , Plant Extracts/pharmacology , Plant Leaves/chemistry , Animal Feed , Animals , Appetite/drug effects , Appetite Stimulants/isolation & purification , Eggs , Feeding Behavior/drug effects , Feeding Behavior/physiology , Female , Hexanes , Larva/drug effects , Larva/physiology , Plant Extracts/analysis , Water , alpha-Linolenic Acid/isolation & purification , alpha-Linolenic Acid/pharmacology , gamma-Linolenic Acid/isolation & purification , gamma-Linolenic Acid/pharmacology
12.
Biosci Biotechnol Biochem ; 74(10): 1987-90, 2010.
Article in English | MEDLINE | ID: mdl-20944433

ABSTRACT

Floral scent has been shown to elicit behavioral responses by butterflies which forage for flowers after receiving appropriate signals. In comparison with investigations about the role of floral scent, those of foliar odor are, however, very few. In this study, the foliar volatiles of Cinnamomum camphora (Lauraceae), which had been collected by air entrainment, exhibited activities toward Graphium sarpedon nipponum (Papilionidae) in both electrophysiological and behavioral tests. The volatiles were analyzed by capillary gas chromatography with electro-antennographic detection (GC-EAD). Two electrophysiological active compounds were found which were determined as nonanal and decanal by using gas chromatography-mass spectrometry (GC-MS). Female butterflies generally tend to show a greater EAG response than males to the headspace volatiles and EAG-active aldehydes. Two EAG-active aldehydes were found in attractant tests to be attractive to both sexes of the butterfly when treated individually. Although the difference between the sexes was not significant, the female butterflies' preference tended to be more active than that of the males.


Subject(s)
Behavior, Animal/drug effects , Cinnamomum camphora/chemistry , Lepidoptera/drug effects , Volatile Organic Compounds/pharmacology , Animals , Biological Assay , Chromatography, Gas , Electrophysiological Phenomena , Female , Lepidoptera/physiology , Male
13.
Z Naturforsch C J Biosci ; 63(9-10): 755-60, 2008.
Article in English | MEDLINE | ID: mdl-19040117

ABSTRACT

Eight compounds isolated from Indian barnyard millet have been identified as L-malic acid, trans-aconitic acid, (+)-isocitric acid, 5-O-caffeoylquinic acid, 4-O-caffeoylquinic acid, isocarlinoside, 2"-O-rhamnosylisoorientin, and 7-O-(2"-O-glucuronosyl)glucuronosyltricin, respectively. These compounds showed high antifeeding activity against brown planthopper only when they were combined.


Subject(s)
Echinochloa/chemistry , Hemiptera/drug effects , Pesticides/toxicity , Animals , Hydrolysis , Isocitrates/isolation & purification , Isocitrates/toxicity , Kinetics , Larva/drug effects , Malates/isolation & purification , Malates/toxicity , Pesticides/isolation & purification , Plant Leaves , Plant Stems , Quinic Acid/isolation & purification , Quinic Acid/toxicity
14.
J Oleo Sci ; 57(2): 93-7, 2008.
Article in English | MEDLINE | ID: mdl-18198465

ABSTRACT

2,3-Dihydro-1H-imidazo[1,2-a]pyridine-4-ylium derivatives with UV absorption at 350 nm were formed by reaction of one molar of any sugar except 2-deoxysugars with two molar of phosphatidylethanolamines involving a new pseudo Maillard rearrangement reaction. To elucidate the reaction mechanism, 2-aminoethyl dihydrogenphosphate, which had a partially similar structural moiety to PE, was reacted with D-galactose. Though the UV absorption at 365 nm was not observed and the four pyridinium derivatives were also not formed in the reactant solution, the UV absorption at 286 nm and browning of the reactant solution were observed. From this reactant solution, two compounds with lambdamax at 283 nm and 297 nm were isolated and former was determined as 5-(hydroxymethyl)furfural (HMF) and later did as phosphoric acid mono{2-[2-formyl-5-(hydroxymethyl)-1H-pyrrol-1-yl]ethyl}ester (PMPEE), which is a new compound, respectively. Because reaction of PMPEE with PE leads to form the pyridinium derivatives, we concluded that a compound like PMPEE was one of intermediates in this new reaction.


Subject(s)
Hot Temperature , Maillard Reaction , Organophosphates/chemistry , Phospholipids/chemistry , Furaldehyde/analogs & derivatives , Furaldehyde/chemistry , Galactose/chemistry , Pyrroles/chemistry , Ultraviolet Rays
15.
Z Naturforsch C J Biosci ; 62(7-8): 603-7, 2007.
Article in English | MEDLINE | ID: mdl-17913080

ABSTRACT

A new cucurbitane glucoside, 23-O-beta-D-glucopyranosyl-7-hydroxy-3-O-malonylcucurbita-5,24-dien-19-al, named momordicine V, has been isolated from Momordica charantia leaves, along with the previously reported compounds, momordicines I, II, IV and 3-O-malonylmomordicine I. The structure of the new compound was established on the basis of spectral analysis, as well as by its conversion to momordicine II by alkaline catalyzed hydrolysis. Momordicine V deterred significantly the oviposition by L. trifolii on host plant leaves treated at 26.16 microg/cm2 leaf surface.


Subject(s)
Glucosides/pharmacology , Glycosides/pharmacology , Momordica charantia/chemistry , Muscidae/physiology , Oviposition/drug effects , Triterpenes/pharmacology , Animals , Female , Glucosides/isolation & purification , Glycosides/isolation & purification , Muscidae/drug effects , Plant Leaves/chemistry , Triterpenes/isolation & purification
16.
J Oleo Sci ; 56(6): 277-81, 2007.
Article in English | MEDLINE | ID: mdl-17898492

ABSTRACT

Novel four 2,3-dihydro-1H-imidazo[1,2-a]pyridine-4-ylium derivatives were obtained with increase of UV absorption at 350 nm and browning of the solution by heating paste lecithin from soybean (SL) in octane. These four derivatives were formed by reaction of one molar of any sugar except 2-deoxysugars with two molar of phosphatidylethanolamines (PE) in SL. To confirm the reaction mechanism, several (13)C-labeled-sugars were reacted with 1,2-di-O-stearoyl-sn-glycero-3-phosphatidylethanolamine (DSPE), respectively. These reactants clearly showed that five carbons of the pyridinium ring and one carbon of the substituted group were based on those of a sugar and that the formation of the pyridinium derivatives was accompanied with cleaving between the carbons of 1- and 2-positions in the sugar and rearrangement. This reaction is a new rearrangement reaction and we named it "new pseudo Maillard rearrangement reaction".


Subject(s)
Carbohydrates/chemistry , Hot Temperature , Phosphatidylethanolamines/chemistry , Magnetic Resonance Spectroscopy , Molecular Structure
17.
Biosci Biotechnol Biochem ; 71(6): 1521-6, 2007 Jun.
Article in English | MEDLINE | ID: mdl-17587684

ABSTRACT

Sweet pepper (Capsicum annuum) leaves at the mature stage have strong ovipositional deterrence against Liriomyza trifolii (Burgess) (Diptera, Agromyzidae), whereas the cotyledons are fiercely attacked by the fly. Treatment of the cotyledons with 50 microM and 100 microM of a jasmonic acid (JA) solution caused the plant to acquire strong oviposition deterrence against the leafminer. An HPLC analysis of the JA-treated cotyledons revealed the inducible accumulation of a compound. Based on spectroscopic analysis and chemical methods, the induced compound was identified to be caffeoylputrescine (CP). The accumulated amounts of CP in the cotyledons treated with 0, 10, 50 and 100 microM of JA were 6.0, 43.0, 105 and 140 microg/g fr. wt., respectively. Treatment of the cotyledons with CP resulted in a significant decrease in the number of punctures made by L. trifolii, indicating that the JA treatment enhanced the deterrence against the leafminer by inducing CP accumulation.


Subject(s)
Cyclopentanes/pharmacology , Diptera/pathogenicity , Insecticides/pharmacology , Plant Diseases/parasitology , Plants/drug effects , Animals , Capsicum , Diptera/drug effects , Dose-Response Relationship, Drug , Oviposition/drug effects , Oxylipins , Plant Leaves , Plants/immunology , Plants/parasitology , Putrescine/analysis
18.
Chem Pharm Bull (Tokyo) ; 55(5): 815-6, 2007 May.
Article in English | MEDLINE | ID: mdl-17473477

ABSTRACT

Two new flavonoid glycosides kaempferol 3-O-beta-D-apiofuranosyl-(1-->6)-beta-D-glucopyranoside (1), and quercetin 3-O-beta-D-apiofuranosyl-(1-->6)-beta-D-glucopyranoside (2), together with six known flavonoid glycosides were isolated from the leaves of Solidago altissima L. grown in Kochi of Japan. The structure elucidation of the isolated compounds was performed by acid hydrolysis and spectroscopic methods including UV, IR, ESI-MS, 1D- and 2D-NMR experiments.


Subject(s)
Disaccharides/chemistry , Flavonoids/chemistry , Glycosides/chemistry , Kaempferols/chemistry , Plant Leaves/chemistry , Quercetin/analogs & derivatives , Solidago/chemistry , Chromatography, High Pressure Liquid , Disaccharides/isolation & purification , Flavonoids/isolation & purification , Glycosides/isolation & purification , Hydrolysis , Kaempferols/isolation & purification , Magnetic Resonance Spectroscopy , Quercetin/chemistry , Quercetin/isolation & purification , Spectrometry, Mass, Electrospray Ionization , Spectrophotometry, Infrared , Spectrophotometry, Ultraviolet , Spectroscopy, Fourier Transform Infrared
19.
Biol Pharm Bull ; 29(12): 2523-7, 2006 Dec.
Article in English | MEDLINE | ID: mdl-17142995

ABSTRACT

Lu xian cao (Hebra pyrolae) is a traditional Chinese medicine used for the treatment of several major diseases. Chimaphilin has been demonstrated one of the major active components in Lu xian cao by modern pharmacological studies. In this study, a liquid chromatography-mass spectrometry method for the determination of chimaphilin in rat plasma was developed and validated. The separation was carried out on a C18 column using methanol and water as mobile phase after the plasma sample was extracted with diethyl ether. Atmospheric pressure chemical ionization in negative ion mode and selected ion monitoring method were developed to determine [M]- at 186 and 210 for chimaphilin and benzil (internal standard), respectively. The lower limit of quantitation was 10 ng/ml and the calibration curve was linear (r>0.9964) over the concentration range 10-1000 ng/ml. The method was demonstrated reproducible and reliable with intra-day precision<11.5%, inter-day precision<7.6%, accuracy in the range of 88.4-113.0%, and mean extraction recovery excess of 83.0%, which were all calculated from the quality control samples at concentrations of 20, 100, and 500 ng/ml. The method was successfully applied to pharmacokinetic study of chimaphilin in rat plasma following oral administration of a 30-mg/kg dose of chimaphilin in Lu xian cao decoction to male Wistar rats.


Subject(s)
Medicine, Chinese Traditional , Naphthoquinones/blood , Animals , Male , Naphthoquinones/pharmacokinetics , Rats , Rats, Wistar , Reference Standards , Reproducibility of Results , Sensitivity and Specificity
20.
Z Naturforsch C J Biosci ; 61(1-2): 81-6, 2006.
Article in English | MEDLINE | ID: mdl-16610222

ABSTRACT

The American serpentine leaf mining fly, Liriomyza trifolii, whose larva feeds on more than 120 plant species is well characterized by its high degree of polyphagy. Observations on the oviposition behavior by L. trifolii demonstrated that among cucurbitaceous plants, Momordica charantia is rarely attacked by L. trifolii. The methanol extract of M. charantia leaves strongly deterred the females from ovipositing on kidney bean leaves treated at a concentration of 1 g leaf equivalent extract/ml. Analysis of the polar fraction of the methanol extract of M. charantia leaves resulted in the isolation of a novel cucurbitane glucoside, 7-O-beta-D-glucopyranosyl-3,23-dihydroxycucurbita-5,24-dien-19-al, named momordicine IV, along with another known compound, momordicine II. Momordicine II and IV deterred oviposition by L. trifolii significantly when bioassays were carried out on kidney bean leaves treated at 75.6 and 20.3 microg/cm2 leaf surface, respectively. There was no synergistic effect on oviposition deterrent when the two compounds were combined in their natural abundance.


Subject(s)
Arthropods/physiology , Glucosides/pharmacology , Momordica charantia/chemistry , Oviposition/drug effects , Amino Acids/isolation & purification , Amino Acids/pharmacology , Animals , Female , Glucosides/isolation & purification , Momordica charantia/growth & development , Plant Leaves/chemistry
SELECTION OF CITATIONS
SEARCH DETAIL
...