ABSTRACT
The effects of a synthetic prostaglandin E1 (PGE1) analog on colony-forming activity in agar cultures of peripheral blood and bone marrow was studied in 28 patients with chronic myeloid leukemia and 9 hematologically healthy subjects. Addition of PGE1 to normal bone marrow culture was followed by a significant drop in the number of colonies per dish in 8 out of the 9 subjects. In leukemic patients, the effect was bizarre. It proved to be in correlation with survival thus suggesting that the effect of PGE1 on colony-forming activity of granulocyte-macrophage precursors be used in predicting survival in patients with chronic myeloid leukemia.
Subject(s)
Leukemia, Myelogenous, Chronic, BCR-ABL Positive/mortality , Alprostadil/pharmacology , Blood Cells/drug effects , Bone Marrow/drug effects , Bone Marrow Cells , Colony-Forming Units Assay , Humans , Leukemia, Myelogenous, Chronic, BCR-ABL Positive/pathology , Prognosis , Tumor Cells, Cultured/drug effects , Tumor Stem Cell AssayABSTRACT
Retrospective analysis of 23 cases of chronic myelomonocytic leukemia (CMML) was presented. Clinical findings, peripheral blood and bone marrow indices, ultrastructural, cytochemical, biochemical (the level of serum and urine lysozyme), cytogenetic investigations as well as the type of leukemic cell growth in culture (monolayer) were considered. Proceeding from the above analysis, the authors found it appropriate to attribute CMML to myelodysplasia.
Subject(s)
Leukemia, Myelomonocytic, Chronic/diagnosis , Aged , Aged, 80 and over , Antineoplastic Combined Chemotherapy Protocols/therapeutic use , Bone Marrow/pathology , Bone Marrow Examination , Chromosome Aberrations/diagnosis , Chromosome Aberrations/pathology , Chromosome Disorders , Female , Humans , Leukemia, Myelomonocytic, Chronic/drug therapy , Leukemia, Myelomonocytic, Chronic/mortality , Leukemia, Myelomonocytic, Chronic/pathology , Male , Middle Aged , Retrospective StudiesABSTRACT
The authors describe 4 families whose members showed myeloproliferative diseases. In one of the families, polycythemia vera (PV) was seen in twin brother and sister, in the other one, chronic myeloleukemia (CML) afflicted both daughter and mother, and in the two remaining families PV and CML afflicted two brothers and mother and daughter, respectively. It was established that neutrophil phosphatase activity was lowered not only in the afflicted brother but also in healthy members of the third family. Based on the reported and their own data the authors arrive at the conclusion that familial myeloproliferative diseases occur in rare cases. In all the cases of familial myeloproliferative diseases, the transmission of the illness by heredity was discovered to be impossible. It was also ascertained that transmitted by heredity are only those cell deficiencies of the tissues that later on will be afflicted by leukemia or will develop immunodeficiency manifested by increased mutation of the myelopoietic cells (DNA repair deficiencies) or by inability to eliminate the leukemic cells.
