ABSTRACT
Pancreatic adenocarcinoma is important in oncology because of its high mortality rate. Deaths may be avoided if an early diagnosis could be achieved. Several types of tumors overexpress gastrin-releasing peptide receptors (GRPr), including pancreatic cancer cells. Thus, a radiolabeled peptide derivative of gastrin-releasing peptide (GRP) may be useful as a specific imaging probe. The purpose of the present study was to evaluate the feasibility of using99mTc-HYNIC-βAla-Bombesin(7-14)as an imaging probe for Capan-1 pancreatic adenocarcinoma. Xenographic pancreatic tumor was developed in nude mice and characterized by histopathological analysis. Biodistribution studies and scintigraphic images were carried out in tumor-bearing nude mice. The two methods showed higher uptake by pancreatic tumor when compared to muscle (used as control), and the tumor-to-muscle ratio indicated that99mTc-HYNIC-βAla-Bombesin(7-14)uptake was four-fold higher in tumor cells than in other tissues. Scintigraphic images also showed a clear signal at the tumor site. The present data indicate that99mTc-HYNIC-βAla-Bombesin(7-14)may be useful for the detection of pancreatic adenocarcinoma.
Subject(s)
Animals , Humans , Male , Adenocarcinoma , Bombesin/analogs & derivatives , Organotechnetium Compounds/pharmacokinetics , Pancreatic Neoplasms , Adenocarcinoma/pathology , Bombesin/pharmacokinetics , Cell Line, Tumor , Gastrin-Releasing Peptide/analogs & derivatives , Heterografts/pathology , Heterografts , Mice, Nude , Muscles , Pancreatic Neoplasms/pathology , Peptide Fragments/pharmacokineticsABSTRACT
Foram testados três métodos de extração de DNA em amostras de queijo, com o objetivo de identificar uma técnica eficiente para extração de DNA em amostras com várias limitações, como alto teor de gordura, alto grau de degradação do DNA e grande concentração de impurezas. A técnica que faz uso do tiocianato de guanidina mostrou-se mais adequada para identificação de adição intencional não declarada de leite bovino em queijos bubalinos, podendo ser empregada para certificação de produto específico (selo de Identidade de Espécie).
Three methods of DNA extraction were tested in cheese samples. The objective of this study was to identify an efficient technique for DNA extraction in different samples with several limitations, such as high fat tenor, high degree of DNA degradation and great sludge concentration. The technique using Guanidine thiocyanate was more appropriate for identification of intentional undeclared addition of bovine milk in buffalo cheeses. This technique can be used for certification of a specific product (stamp of Identity of Species).
ABSTRACT
The larger palatine foramen (FPM), for being the emerging point of the vasculo neural bundle that it isdistributed to the hard palate, for being close the area that donates gingival grafts, for being area for performinganesthetic techniques for desensitization of the hard palate, and still, for being the point of entrance of thepalatine channel for reaching the pterigopalatine ganglion, becomes fundamental in oral surgery interventions.141 softened human craniums were studied, from which 82 were masculine craniums and 59 were femininecraniums. The measures were collected with a Mitutoyo precision pachymetry, and a compass, among thereference points previously standardized. The collected data were submitted to the statistical analysis. Theresults of this research demonstrated that there was a significant difference between the measures of right andleft FPMs in relation to the interpalatine suture. In most of the craniums, the FPM was located closer to thethird molar. The masculine craniums presented dimensions significantly larger than the feminine craniumsand, except for the distance between left FPM and the incisive foramen, all the other distances from the FPMto the points taken as reference can be considered to be considered in order to estimate the sex. It can beconcluded that the results found diverged in relation to some of the literature reviewed. Some new studiedare suggested, mainly studies that consider the incisive foramen and the maxillary tuber as reference points todefine the topography of the larger palatine foramen.
