Volumetric Evaluation of Voids and Gaps of Different Calcium-Silicate Based Materials Used in Furcal Perforations: A Micro-CT Study.

This study aimed at evaluating volumetrically gaps and voids of calcium-silicate based materials of different generations and handling properties (BC­Endosequence BC RRM-Fast Set Condensable Putty, MTA­ProRoot MTA, and BIO­Biodentine) in simulated furcal perforations in an ex vivo setup by microcomputed tomography (Micro-CT) analysis. Thirty-six extracted human mandibular molars with sound furcation areas were selected. Standardized perforations were created in the furcation area of the pulp chamber using #4 diamond burs. The specimens were randomly assigned to three groups (BC, MTA and BIO; n = 12). Samples were then scanned (SkyScan 1172; Bruker-microCT, Kontich, Belgium), and three-dimensional (3D) images reconstructed. The relative volume of gaps (VG%) and voids (VV%) present on each material was calculated. Data were analyzed using one-way analysis of variance (ANOVA) and Tukey's HSD test (p < 0.05). Mean VG% for BC, MTA, and BIO groups were, respectively, 0.513%, 1.128%, 1.460%, with BC presenting statistically (p < 0.05) fewer gaps formation than the other groups. Mean VV% were, respectively, 0.018%, 0.037%, and 0.065%. The was no statistical difference regarding VV%. There were no gap-free and void-free samples. BC group had the lowest VG% among the groups with a significant statistical difference (p < 0.05).

Filling ability of three bioceramic root-end filling materials: A micro-computed tomography analysis.

This study aimed at evaluating the volume of gaps and voids, and the total porosity percentage of three calcium-silicate-based materials in mandibular molars apicoectomy by Micro-CT analysis. Thirty-three mesial roots of extracted human mandibular molars were instrumented and obturated. The apical 3mm of each root was resected and prepared. Root-end cavities were filled with EndoSequence BC Putty (BC); ProRoot MTA (MTA) and Biodentine (BIO). Samples were scanned using a Micro-CT scanner and the tridimensional images reconstructed. Percentage of gaps (VG%) and of voids (VV%) were obtained. Porosity percentage (Po%) was also assessed. Data were analysed using Student's t-test (P < 0.05). All materials presented gaps and voids. VG% was 2.006 (BC), 1.882 (MTA) and 1.450 (BIO), and VV% was 0.039 (BC), 0.021 (MTA) and 0.024 (BIO) with no statistical difference. Po% were 56.73 (BC), 51.94 (MTA) and 50.45 (BIO), with BC being statistically (P > 0.05) more porous.

Bond strength of different endodontic sealers to dentin: push-out test

OBJECTIVE: The aim of this in vitro study was to evaluate the bond strength of different root canal sealers to dentin. MATERIAL AND METHODS: Forty extracted single-rooted human teeth were examined and the coronal and middle thirds of the canals were prepared with a 1.50 mm post drill (FibreKor Post System, Pentron). The teeth were allocated in two experimental groups, irrigated with 2.5 percent NaOCl+17 percent EDTA or saline solution (control group) and instrumented using Race rotary files (FKG) to a size #40 at the working length. Then, the groups were divided into four subgroups and filled with Epiphany sealer (Group 1), EndoREZ (Group 2), AH26 (Group 3) and Grossman's Sealer (Group 4). After 2 weeks of storage in 100 percent humidity at 37ºC, all teeth were sectioned transversally into 2-mm-thick discs. Push-out tests were performed at a cross-head speed of 1 mm/min using a universal testing machine. The maximum load at failure was recorded and expressed in MPa. RESULTS: Means (±SD) in root canals irrigated with 2.5 percent NaOCl and 17 percent EDTA were: G1 (21.6±6.0), G2 (15.2±3.7), G3 (14.6±4.5) and G4 (11.7±4.1).Two-way ANOVA and Tukey's test showed the highest bond strength for the Epiphany's group (p< 0.01) when compared to the other tested sealers. Saline solution decreased the values of bond-strength (p<0.05) for all sealers. CONCLUSION: Epiphany sealer presented higher bond strength values to dentin in both irrigating protocols, and the use of 2.5 percent NaOCl and 17 percent EDTA increased the bond strength values for all sealers.

Bond strength of different endodontic sealers to dentin: push-out test.

OBJECTIVE: The aim of this in vitro study was to evaluate the bond strength of different root canal sealers to dentin. MATERIAL AND METHODS: Forty extracted single-rooted human teeth were examined and the coronal and middle thirds of the canals were prepared with a 1.50 mm post drill (FibreKor Post System, Pentron). The teeth were allocated in two experimental groups, irrigated with 2.5% NaOCl+17% EDTA or saline solution (control group) and instrumented using Race rotary files (FKG) to a size #40 at the working length. Then, the groups were divided into four subgroups and filled with Epiphany sealer (Group 1), EndoREZ (Group 2), AH26 (Group 3) and Grossman's Sealer (Group 4). After 2 weeks of storage in 100% humidity at 37ºC, all teeth were sectioned transversally into 2-mm-thick discs. Push-out tests were performed at a cross-head speed of 1 mm/min using a universal testing machine. The maximum load at failure was recorded and expressed in MPa. RESULTS: Means (±SD) in root canals irrigated with 2.5% NaOCl and 17% EDTA were: G1 (21.6±6.0), G2 (15.2±3.7), G3 (14.6±4.5) and G4 (11.7±4.1).Two-way ANOVA and Tukey's test showed the highest bond strength for the Epiphany's group (p< 0.01) when compared to the other tested sealers. Saline solution decreased the values of bond-strength (p<0.05) for all sealers. CONCLUSION: Epiphany sealer presented higher bond strength values to dentin in both irrigating protocols, and the use of 2.5% NaOCl and 17% EDTA increased the bond strength values for all sealers.

In vitro assessment of the immediate and prolonged antimicrobial action of chlorhexidine gel as an endodontic irrigant against Enterococcus faecalis.

OBJECTIVE: The aim of this study was to assess in vitro the antimicrobial activity of 2% chlorhexidine gel against Enterococcus faecalis , comparing it to other endodontic irrigants (2% chlorhexidine liquid and 5.25% sodium hypochlorite). STUDY DESIGN: Eighty roots of human mandibular premolars were prepared by serial instrumentation technique, autoclaved, and contaminated for 7 days with E faecalis monocultures. The roots were then divided into 5 groups according to the irrigant substance used during the standardized biomechanical preparation. In order to evaluate the antimicrobial action of the irrigant substances, 3 microbial samples were taken: initial (before the biomechanical preparation); post-treatment (immediately after the biomechanical preparation), and final (7 days after the biomechanical preparation). The microbiological samples were plated to count the colony-forming units (CFU). RESULTS: The 2% chlorhexidine gel and 2% chlorhexidine liquid significantly reduced the E faecalis CFU in the post-treatment and final microbiological samples. The 5.25% sodium hypochlorite also reduced the E faecalis CFU immediately after the root canal instrumentation, but it was not able to keep the root canal free of detectable E faecalis in the final sample. CONCLUSIONS: The 2% chlorhexidine gluconate (gel and liquid) antimicrobial ability was more effective than 5.25% sodium hypochlorite in keeping low CFU of E faecalis for 7 days after the biomechanical preparation.

An evaluation of microbial leakage in roots filled with a thermoplastic synthetic polymer-based root canal filling material (Resilon).

The purpose of this study was to compare bacterial leakage using Streptococcus mutans and Enterococcus faecalis through gutta-percha and a thermoplastic synthetic polymer-based root filling (Resilon) using two filling techniques during a 30-day period. Teeth were decoronated, roots prepared to a length of 16 mm, and instrumented to ISO sizes 40 to 50. A total of 156 roots were randomly divided into 8 groups of 15 roots (groups 1-8) and 3 control groups (12 roots each). Roots were filled using lateral and vertical condensation techniques with gutta-percha and AH 26 sealer (groups 1 and 2) or with gutta-percha and Epiphany sealer (groups 3 and 4). Groups 5 and 6 were filled with Resilon and Epiphany sealer using the lateral or vertical condensation techniques. A split chamber microbial leakage model was used in which S. mutans placed in the upper chamber could reach the lower chamber only through the filled canal. Groups 7 and 8 were identical to groups 5 and 6 respectively; however, E. faecalis was used to test the leakage. Positive controls were filled with Resilon (12 roots) and gutta-percha (12 roots) without sealer and tested with bacteria, whereas negative controls (12 roots) were sealed with wax to test the seal between chambers. All but one positive control leaked within 24 h, whereas none of the negative controls leaked. Resilon showed minimal leakage (group 8: one leakage; groups 5-7: each with two leakages), which was significantly less than gutta-percha, in which approximately 80% of specimens with either technique or sealer leaked. Kruskal-Wallis test showed statistical significance when all groups were compared (p < 0.05). Mann-Whitney U test compared the respective groups and found Resilon groups superior to gutta-percha groups (p < 0.05).

Fatores de sucesso em endodontia: análise retrospectiva de 2000 casos clínicos / Success evaluation of 2000 endodontically treated teeth: a retrospective study

Este trabalho retrospectivo avaliou o índice de sucesso de 2.000 tratamentos e retratamentos endodônticos realizados por um especialista. As informações obtidas nos controles clínico-radiográficos foram transformadas em porcentagem e examinadas estatisticamente usando o teste qui-quadrado de Pearson e o teste exato de Fisher. A média geral de sucesso foi de 91,45 por cento, e os resultados mostraram que polpa com vitalidade, ausência de lesão, modalidade de intervenção convencional, nível de obturação aquém-ápice e sessão única apresentaram índices de sucesso estatisticamente maiores do que polpas sem vitalidade, presença de lesão, retratamento, obturações no limite radiográfico ou além-ápice e sessões múltiplas

Bacteriological study of root canals associated with periapical abscesses.

OBJECTIVE: The aim of this study was to identify microorganisms from root canals with periapical abscesses and to ascertain the susceptibility of Peptostreptococcus prevotii and Fusobacterium necrophorum to antimicrobials. Study design Thirty root canals were microbiologically sampled by using sterile paper points. The concomitant microorganisms were identified through the use of established methods. The susceptibility of P prevotii and F necrophorum to antimicrobials was evaluated by using the E test method. RESULTS: A total of 117 different bacterial strains were recovered, including 75 strict anaerobes or microphilic species. The most frequently isolated strict anaerobes were P prevotii, Peptostreptococcus micros, and F necrophorum. Facultative bacteria such as Gemella morbillorum and Streptococcus mitis were also found, albeit less frequently. The data revealed that P prevotii and F necrophorum were susceptible to the tested antibiotics. CONCLUSIONS: Gram-positive anaerobic bacteria predominate in the mixed microbiota of root canals with periapical abscesses. Moreover, P prevotii and F necrophorum are susceptible to the tested antibiotics.

Microbial susceptibility to calcium hydroxide pastes and their vehicles.

The aim of this study was to investigate the susceptibility of some microorganisms commonly isolated from root canals to calcium hydroxide in combination with several vehicles by the agar diffusion method. Stainless-steel cylinders were placed on each inoculated agar medium. The test medications and their controls were placed inside the cylinders. The zones of growth inhibition were measured and recorded after the incubation period for each plate, and the results were analyzed statistically. Enterococcus faecalis was most resistant, whereas the anaerobic Porphyromonas endodontalis was more susceptible to all medications, followed by P. gingivalis and Prevotella intermedial intermedia. Ca(OH)2 + CMCP + glycerin showed significantly larger mean zones of inhibition when compared with the other medications. We conclude that anaerobic Gram-negative bacteria are more susceptible to calcium hydroxide pastes than facultative Gram-positive microorganisms.

In vitro antimicrobial activity of calcium hydroxide pastes and their vehicles against selected microorganisms.

The aim of this study was to investigate in vitro the antimicrobial activity of calcium hydroxide in combination with several vehicles against some microorganisms commonly isolated from root canals. Antimicrobial activity was determined by the agar diffusion method. Stainless-steel cylinders were placed on each inoculated agar medium. The test medicaments and their controls were placed inside the cylinders. The zones of growth inhibition were measured and recorded after incubation for each plate and the results were analyzed statistically (ANOVA). The in vitro antimicrobial effects of the medications were ranked from strongest to weakest as follows: Ca(OH)2 + CMCP + glycerine, Ca(OH)2 + CMCP, Ca(OH)2 + glycerine, Ca(OH)2 + anesthetic, Ca(OH)2 + saline, Ca(OH)2 + H2O, Ca(OH)2 + polyethyleneglycol. The pastes with oily vehicles showed significantly larger mean zones of inhibition compared to those with aqueous or viscous vehicles. It was concluded that diffusion and antimicrobial activity of calcium hydroxide were affected by the type of vehicle used.

Effect of sonic and ultrasonic retrograde cavity preparation on the integrity of root apices of freshly extracted human teeth: scanning electron microscopy analysis.

Sonic and ultrasonic root-end preparation devices permit the preparation of conservative and straight cavities. However, microfractures and marginal chipping can occur due to the vibratory action of such instruments. The purpose of this study was to evaluate the effect of retrograde preparations by using sonic and ultrasonic tips on the integrity of root-end surfaces. Eighty, human anterior teeth were grouped according to size and treated as follows: treatment 1: root-end resection (RR) and root-end cavity preparation (RP) with KIS ultrasonic retrotip; treatment 2: RR and RP with Satelec S12/90 (no diamond coating) ultrasonic retrotip; treatment 3: RR and RP with a diamond-coated retrotip attached to a sonic device (Sonics); treatment 4: RR and RP with Satelec S12/90D (diamond coated) ultrasonic retrotip. The root-end surface topographies were assayed by means of polyvinylsiloxane impressions and epoxy resin replicas. SEM pictures of each sample were taken before and after preparation, and the images were evaluated by using an image processing and analysis system. The parameters evaluated were shape quality, presence of cracks, and marginal chipping. The results showed no statistically significant differences among anatomic groups, treatments, or tooth sizes (p > 0.05). Because treatment 3 removed more dental structure than the others did, its use on small teeth is inadvisable.

Reattachment of an autogenous tooth fragment in a fracture with biologic width violation: a case report.

An 11-year-old patient that fractured her maxillary left central incisor is presented. The fracture involved two thirds of the crown, compromising the pulp, and extended subgingivally on the palatal aspect, invading the biologic width. The procedure used to repair the fracture included flap surgery with a slight ostectomy and endodontic treatment. The reattachment of the tooth fragment and the restoration were performed with a bonding system and a resin composite. Examination 6 months after treatment revealed periodontal health, good esthetics, and normal function.

In vitro antimicrobial activity of calcium hydroxide pastes and their vehicles against selected microorganisms

The aim of this study was to investigate in vitro the antimicrobial activity of calcium hydroxide in combination with several vehicles against some microorganisms commonly isolated from root canals. Antimicrobial activity was determined by the agar diffusion method. Stainless-steel cylinders were placed on each inoculated agar medium. The test medicaments and their controls were placed inside the cylinders. The zones of growth inhibition were measured and recorded after incubation for each plate and the results were analyzed statistically (ANOVA). The in vitro antimicrobial effects of the medications were ranked from strongest to weakest as follows: Ca(OH)2 + CMCP + glycerine, Ca(OH)2 + CMCP, Ca(OH)2 + glycerine, Ca(OH)2 + anesthetic, Ca(OH)2 + saline, Ca(OH)2 + H2O, Ca(OH)2 + polyethyleneglycol. The pastes with oily vehicles showed significantly larger mean zones of inhibition compared to those with aqueous or viscous vehicles. It was concluded that diffusion and antimicrobial activity of calcium hydroxide were affected by the type of vehicle used

Remoçäo de Smear Layer dos canais radiculares utilizando o irrigante Endoquil / Evaluation of the Smear Layer removal in root canals using Endoquil irrigant

O trabalho teve o objetivo de avaliar a capacidade de remoçäo de smear layer dos canais radiculares empregando diferentes tipos de irrigantes endodônticos. Foram utilizados 20 dentes, distribuídos entre quatro grupos: EDTA 17 por cento; hipoclorito de sódio a 5,25 por cento; Endoquil©; tendo a água destilada como grupo controle. Os dentes foram instrumentados e clivados, sendo as amostras submetidas à análise em microscopia eletrônica de varredura. Os resultados mostraram que os grupos do EDTA 17 por cento e Endoquil© obtiveram superior capacidade de limpeza das paredes dos canais radiculares

Descontaminaçäo química de cones de guta-percha por diferentes concentraçöes de NaOCl / Chemical decontamination of gutta-percha cones by different concentrations of NaOCl

Neste experimento foi testada a efetividade de 5 diferentes concentraçöes de hipoclorito de sódio (0,5 percent, 1 percent, 2,5 percent, 4 percent e 5,25 percent) na descontaminaçäo de cones de guta-percha artificialmente contaminados por diferentes tipos de microrganismos. Também foi determinado o período ótimo de imersäo necessário para atingir esse efeito. Nenhum dos microrganismos cresceu a partir de 45s de exposiçäo ao NaOCl a 5,25 por cento e a partir de 30min de exposiçäo ao NaOCl a 0,5 percent. Os testes microbiológicos indicam que o NaOCl é um método efetivo de descontaminaçäo dos cones de guta-percha e o período de imersäo necessário para atingir esse efeito é inversamente proporcional ao aumento da concentraçäo

Clareamento dental interno com pasta de perborato de sódio e água destilada / Internal dental bleaching using sodium perborate/distilled water paste

O clareamento dental interno, realizado em dentes tratados endodonticamente, tem sido aplicado rotineiramente na clínica odontológica como excelente alternativa a procedimentos restauradores. O presente trabalho tem como objetivo revisar os materiais empregados na técnica de clareamento "walking bleach", bem como sua evoluçäo com o passar dos anos. Os autores discutem o tratamento com uso da pasta de perborato de sódio/água, descrevendo um protocolo seguro e eficiente