ABSTRACT
In the present investigation, pulsatile release beads were prepared by ionic gelation technique. Theophylline dual-cross-linked beads were prepared by dropping dispersed phase of theophylline, Delonix regia gum (DRG), and sodium alginate into the dispersion phase of different concentration of calcium chloride solution followed by aluminium chloride solution. The formulated beads were further coated by Eudragit L & S 100 in the ratio 1:2 w/w in order to achieve desired lag time. In vitro release study showed lag time of 57 h before release of theophylline from the formulated beads, which were found to be intact for 6 h. Thus, formulated dual cross-linked beads when administered at bed time may release theophylline when needed most for chronotherapeutics of early morning asthmatic attacks in chronic patients. In vivo radio imaging study carried out in New Zealand white strain rabbit confirms the findings of in vitro results.
Subject(s)
Asthma/drug therapy , Chronotherapy , Theophylline , Alginates/chemistry , Alginates/pharmacology , Animals , Biocompatible Materials/chemistry , Biocompatible Materials/pharmacology , Bronchodilator Agents/pharmacology , Chemistry, Pharmaceutical , Delayed-Action Preparations/pharmacology , Drug Carriers , Drug Delivery Systems , Drug Evaluation, Preclinical , Excipients , Fabaceae/chemistry , Glucuronic Acid/chemistry , Glucuronic Acid/pharmacology , Hexuronic Acids/chemistry , Hexuronic Acids/pharmacology , Humans , Mannose/chemistry , Mannose/pharmacokinetics , Microspheres , Polymethacrylic Acids/chemistry , Polymethacrylic Acids/pharmacology , Rabbits , Theophylline/pharmacologyABSTRACT
Fixed dose combination tablets containing ambroxol HCl and cetirizine HCl are clinically used as mucolytic and antiallergic. Several spectrophotometric and HPLC methods have been reported for simultaneous estimation of these drugs with other drugs. The drugs individually and in mixture obeys Beer's law over conc. range 1.2-4.4 microg/mL for cetirizine HCL and for ambroxol HCL 15-52 microg/mL at all five sampling wavelengths (correlation coeff. well above 0.995). The mean recoveries from tablet by standard addition method were 100.18% (+/-2.4) and 100.66 % (+/-2.31). The present work reports simple, accurate and precise spectrophotometric methods for their simultaneous estimation from tablet dosage form.
