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1.
Pharm Biol ; 53(6): 824-30, 2015 Jun.
Article in English | MEDLINE | ID: mdl-25720975

ABSTRACT

CONTEXT: Recently, mushrooms are interesting natural products to be investigated due to exhibiting various bioactivities. OBJECTIVE: This study determines the antioxidant and anticholinesterase activities of various extracts of five wild mushroom species. In addition, the total bioactive contents, namely, ascorbic acid, ß-carotene, and lycopene along with phenolic and flavonoid contents were also determined spectrophotometrically. MATERIALS AND METHODS: Antioxidant activity was tested by using five complementary tests; namely, ß-carotene-linoleic acid, DPPH(•) scavenging, ABTS(•+) scavenging, cupric-reducing antioxidant capacity (CUPRAC), and metal chelating assays. The in vitro anticholinesterase activity was tested against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes using the Ellman method. The spectrophotometric methods were used to determine the total phenolic, flavonoid, ascorbic acid, ß-carotene, and lycopene contents. RESULTS: The current study has shown that ethyl acetate extracts of Ganoderma lucidum (Curtis) P. Karst (IC50: 1.55 ± 0.05 µg/mL) and Funalia trogii (Berk.) Bondartsev & Singer (IC50: 4.31 ± 0.18 µg/mL) exhibited good lipid peroxidation inhibitory activity. The DPPH, ABTS, and CUPRAC assays supported this activity. The ethyl acetate and methanol extracts of Funalia trogii and Ganoderma lucidum indicated good anticholinesterase activity. Ganoderma lucidum had rich phenolic and flavonoid contents, indicating 98.67 ± 0.32 mg PEs/g extract and 160.38 ± 1.25 mg QEs/g extract, respectively. DISCUSSION AND CONCLUSION: The results demonstrate that some of the mushroom species tested herein could be used in food and pharmaceutical industries as natural antioxidants.


Subject(s)
Agaricales/chemistry , Antioxidants/pharmacology , Cholinesterase Inhibitors/pharmacology , Acetylcholinesterase/metabolism , Butyrylcholinesterase/metabolism , Chelating Agents/pharmacology , Copper/chemistry , Free Radical Scavengers/chemistry , Oxidation-Reduction
2.
Article in English | MEDLINE | ID: mdl-25029407

ABSTRACT

Mineral and metal contents of 24 wild mushroom species collected from Anatolia were analysed by inductively coupled plasma optical emission spectrometry. Eight minerals (Na, Mg, Ca, Mn, Fe, Zn, P and K) and six metals (Cr, Ni, Ag, Co, Cu and Pb) were studied. The mineral content of mushroom samples ranged 77.1-1061.2 mg/kg for Na, 268.1-1927.9 mg/kg for Mg, 19.3-352.9 mg/kg for Ca, 1.23-75.36 mg/kg for Mn, 27.8-816.1 mg/kg for Fe, 1.61-122.13 mg/kg for Zn, 176.7-5726.4 mg/kg for P and 1133.3-9866.7 mg/kg for K. The metal content ranged 0.03-10.58 mg/kg for Cr, 0.24-48.65 mg/kg for Ni, 0.02-0.63 mg/kg for Ag, 0.02-5.13 mg/kg for Co, 1.10-9.04 mg/kg for Cu and 0.07-8.46 mg/kg for Pb.


Subject(s)
Agaricales/chemistry , Food Contamination/analysis , Metals, Heavy/analysis , Minerals/analysis , Nutritive Value , Species Specificity , Turkey
3.
Food Chem Toxicol ; 48(11): 3189-93, 2010 Nov.
Article in English | MEDLINE | ID: mdl-20732375

ABSTRACT

The essential oil and methyl ester of hexane extract of Salvia chionantha Boiss. were analysed by GC and GC-MS. Totally, 54 components were detected in the essential oil and all of them were fully determined. Germacrene D (25.03%), ß-caryophyllene (8.71%), spathulenol (5.86%) and α-humulene (4.82%) were identified as the major compounds. In the methylated hexane extract, 3-hydroxy hexadecanoic acid (39.39%), 3-hydroxy tetradecanoic acid (12.66%) and palmitic acid (12.02%) were the major fatty acids elucidated. The antioxidant activity of the essential oil and the hexane extract was determined by using four complementary test systems; namely, ß-carotene-linoleic acid, DPPH() scavenging, ABTS(+)* scavenging, and CUPRAC assays. In ß-carotene-linoleic acid assay, the extract showed 81.2±0.1% lipid peroxidation inhibition at 0.8 mg/mL concentration, while in ABTS(+)* assay the essential oil exhibited 77.4±0.5% inhibition at same concentration. Since, acetylcholinesterase and butyrylcholinesterase enzymes are taking place in pathogenesis of Alzheimer's disease, in vitro anticholinesterase activity of the essential oil and the extract was also studied spectrophotometrically. At 0.5mg/mL concentration, the essential oil showed moderate acetylcholinesterase (56.7±1.9%) and butyrylcholinesterase (41.7±2.9%) inhibitory activity, while the extract was only exhibited activity (63.1±0.8%) against butyrylcholinesterase enzyme. Hence, the essential oil may be useful as a moderate anticholinesterase agent, particularly against acetylcholinesterase.


Subject(s)
Antioxidants/pharmacology , Cholinesterase Inhibitors/pharmacology , Oils, Volatile/pharmacology , Plant Oils/pharmacology , Salvia/chemistry , Acetylcholinesterase/analysis , Acetylcholinesterase/metabolism , Antioxidants/chemistry , Biphenyl Compounds/metabolism , Butyrylcholinesterase/analysis , Butyrylcholinesterase/metabolism , Copper/metabolism , Free Radical Scavengers/metabolism , Free Radicals/metabolism , Gas Chromatography-Mass Spectrometry , Hexanes/chemistry , Indicators and Reagents/metabolism , Linoleic Acid/chemistry , Oils, Volatile/chemistry , Oxidation-Reduction , Picrates/metabolism , Plant Oils/chemistry , beta Carotene/chemistry
4.
Nat Prod Commun ; 4(7): 1017-20, 2009 Jul.
Article in English | MEDLINE | ID: mdl-19731615

ABSTRACT

The chemical compositions of the essential oils of Salvia potentillifolia (Lamiaceae) were analyzed by GC and GC-MS. The oils were obtained by four different methods (hydrodistillation, steam distillation, and two extraction methods) to investigate the effect of temperature on the volatile compounds. In total, 121 components were detected in the various oils. The major compounds characterized were alpha-pinene (30.2, 31.2, 10.6, and 14.8%) and beta-pinene (15.0, 14.6, 7.6, and 11.4%), respectively. Surprisingly, the percentage concentrations of alpha-pinene and beta-pinene in the hydrodistilled and steam distilled oils were high, even though prepared at high temperature, whereas the contents of both compounds were lower in the essential oils obtained by the extraction methods prepared without added heat. The percentage concentration of 8,13-epoxy-labda-14-en-2-one, a diterpenoid, in the extracted essential oil was 22 times higher than in the hydrodistilled and steam distilled essential oils. However, the concentrations of cis-p-menth-2-en-1-ol was four times, and beta-bourbonene and beta-caryophyllene two times superior than their percentage concentrations in the hydrodistilled and steam distilled essential oils.


Subject(s)
Oils, Volatile/chemistry , Salvia/chemistry , Gas Chromatography-Mass Spectrometry , Hexanes/chemistry , Solvents , Temperature , Turkey
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