[Comparative studies of paramyxovirus isolates from wild birds]. / Vergleichende Untersuchungen an Paramyxovirus-Isolaten aus Wildvögeln.

As a result of a surveillance programme in North-Germany, paramyxovirus-isolates of serogroup 1 with different pathogenicity were isolated from different species of feral birds (Black-headed gull, mallard, tawny owl, tree sparrow, mute swan). In chick embryo fibroblast monolayers these isolates produced plaques exhibiting different plaque morphology. After cloning the isolates, further physicochemical, serological and pathogenicity testings were performed. On the basis of mean death time and intracerebral pathogenicity index, two of the isolates were found to be avirulent (lentogenic), whereas the others proved to be virulent (velogenic). SDS-Polyacrylamide-gel-electrophoresis showed polypeptide patterns similar to that of the PMV-1 reference strains. Summarizing all relevant findings of these investigations under consideration of the suggestions for the virological description of avian PMV (Alexander, 1987; Alexander et al., 1987; Who-Expert-Committee, 1980), including the recently established serotyping by means of monoclonal antibodies, the following designations of the PMV isolates tested herein are proposed: 1. PMV-1/black-headed gull/Germany/SSP-233/83, lentogenic NDV, monoclonal antibody group G 2. PMV-1/mallard/Germany/SSP-284/83, lentogenic NDV, monoclonal antibody group H 3. PMV-1/tawny owl/Germany/SSP-262/84, velogenic NDV, monoclonal antibody group B 4. PMV-1/tree sparrow nestling/Germany/SSP-352/84, velogenic NDV, monoclonal antibody group B 5. PMV-1/mute swan/Germany/SSP-204/85, velogenic NDV, monoclonal antibody group B.

[The occurrence of paramyxoviruses in game birds: epizootiological aspects, characteristics in vivo and in vitro. A review of the literature]. / Vorkommen von Paramyxoviren bei Wildvögeln: Epizootiologische Aspekte, Eigenschaften in vivo und in vitro.--Eine Literaturübersicht.

The recent review is based on recent literature on the epizootiology and on properties of avian paramyxoviruses in vivo and in vitro. Paramyxoviruses are classified according to their pathogenicity (especially serotype 1), in addition to serotyping by means of hemagglutination-inhibition tests and by monoclonal antibody binding assays. Also, neuraminidase-inhibition tests, double-immunodiffusion tests can be performed. Growth properties of the different paramyxovirus-serotypes are accessory techniques to classify paramyxoviruses parallel to the determination of the pathogenicity. Structural polypeptides can be demonstrated in polyacrylamide-electrophoresis, thus leading to a distinction of the different serotypes.