ABSTRACT
Most drug discovery efforts for Alzheimer's disease (AD) have focused on prevention or clearance of beta-amyloid (Abeta) fibrils or oligomers, with far less attention to prevention of tau abnormalities that lead to neurofibrillary tangles (NFTs). Much evidence now indicates that Abeta multimers can trigger neurodegenerative changes that involve formation of dystrophic neurites and cytoskeletal collapse, possibly due loss of microtubule (MT) stabilization by the tau protein. We have found that several MT-stabilizing agents such as Taxol significantly enhanced neuronal survival in the presence of Abeta and identified agents that enter the brain, a necessity for in vivo testing in animal models of tau pathology. Studies were designed to test two agents in the tau mutant (JNPL3) mouse that develops severe motor deficits at about seven months of age, accompanied by neuropathological markers of tau pathology. In addition to using motor performance tests through the planned period of drug administration, we designed a simple appetitive memory test that required a reduction in ad lib food intake. Although the neurochemical data are still being analyzed, we were surprised to find that all of the JNPL3 mice, whether receiving the drug or not, developed no signs of motor impairment up to 10 months of age. This is considerably beyond the age at which free-fed mice survived and suggests that the food restriction alone may have delayed the pathological process. A study is ongoing with free-fed mice to determine if the drug interventions do have any beneficial effects in these mutant mice.
Subject(s)
Brain/drug effects , Drug Evaluation, Preclinical/methods , Microtubules/drug effects , Paclitaxel/pharmacology , tau Proteins/drug effects , Alzheimer Disease/drug therapy , Animals , Association Learning/drug effects , Blood-Brain Barrier/metabolism , Brain/metabolism , Caloric Restriction , Mice , Mice, Transgenic , Paclitaxel/analogs & derivatives , Paclitaxel/pharmacokinetics , Reinforcement, Psychology , Rotarod Performance TestABSTRACT
Recently it has become increasingly clear that chemicals found in our foods and beverages can prevent the genetic damage that leads to cancer initiation. Such substances may also affect subsequent events in the pathways that lead to cancer, and may have the potential to inhibit the mutations that allow tumor cells to become resistant to antitumor agents. We describe here the antimutagenic potential of Glabrene analogs against EMS-induced mutations utilizing modified Ames tests in S. typhimurium TA 100 and E. coli JC 5088. Results of studies of the ability of well-known antioxidants such as EGCG and related compounds to prevent drug resistance mutations in microorganisms are described, and their possible significance in the prevention of chemotherapeutic drug-resistance in tumor cells is discussed.
Subject(s)
Anticarcinogenic Agents/pharmacology , Catechin/analogs & derivatives , Neoplasms/prevention & control , Antimutagenic Agents/pharmacology , Antioxidants/pharmacology , Catechin/pharmacology , Drug Resistance, Microbial , Escherichia coli/drug effects , Escherichia coli/genetics , Histidine , Mutagenicity Tests , Mutation/drug effects , Salmonella typhimurium/drug effects , Salmonella typhimurium/genetics , Tea/chemistryABSTRACT
Berberine (4) is responsible for the activity of an extract of a commercial root sample of Hydrastis canadensis against multiply drug resistant Mycobacterium tuberculosis. Two new quinic acid feruloyl esters, compounds 2 and 3, have been isolated from the same source along with canadine (1c), 8-oxotetrahydrothalifendine (1), and beta-hydrastine (5). These were found to be inactive. The structures of the new compounds were elucidated from spectral (1H, 13C, HMQC, HMBC, and H-H COSY) and chemical evidences.
Subject(s)
Antitubercular Agents/pharmacology , Berberine/pharmacology , Plants, Medicinal/chemistry , Alkaloids/isolation & purification , Antitubercular Agents/chemistry , Benzylisoquinolines , Berberine/analogs & derivatives , Berberine/isolation & purification , Biological Products/chemistry , Biological Products/isolation & purification , Biological Products/pharmacology , Magnetic Resonance Spectroscopy , Microbial Sensitivity Tests , Plant Roots/chemistry , Quinic Acid/analogs & derivatives , Quinic Acid/isolation & purificationABSTRACT
The literature of combinatorial chemistry is reviewed with particular attention paid to considerations of absorption, distribution, metabolism and excretion in the design and evaluation of libraries containing drug-like molecules. Published libraries are evaluated in particular for the likelihood that the products would possess oral bioavailability.
Subject(s)
Chemistry, Pharmaceutical/methods , Drug Design , Administration, Oral , Biological Availability , Pharmacokinetics , Structure-Activity RelationshipABSTRACT
Following a brief review of recent discoveries in the field of natural antimutagenic and tumor chemopreventive agents, contemporary findings in the author's laboratories employing the direct acting mutagen, ethyl methanesulfonate, in modified Ames tests and eukaryotic murine FM3A mammary tumor cells modified to be subject to thymidine-less death are described to illustrate the underlying principles. The EMS studies are illustrated with the isolation of the novel antimutagen, plicatin B, from the medicinal plants, Psoralea juncaea and P. plicata. The FM3A studies are carried out with extracts of Styrax asiatica, a plant previously studied extensively with the EMS system. The FM3A findings closely parallel the earlier work with EMS showing that the responsible agents, cinnamic acid, cinnamoyl ricinoleate and cinnamoyl cinnamate are effective both in prokaryotic and eukaryotic tests and that the new FM3A assay system has useful properties for screening and assay of novel antimutagenic agents.
Subject(s)
Antimutagenic Agents/pharmacology , Plant Extracts/pharmacology , Acrylates/isolation & purification , Acrylates/pharmacology , Animals , Anticarcinogenic Agents/pharmacology , Antimutagenic Agents/chemistry , Drug Evaluation, Preclinical , Ethyl Methanesulfonate/toxicity , Female , Mammary Neoplasms, Experimental , Mice , Mice, Inbred Strains , Mutagenicity Tests , Mutagens/toxicity , Tumor Cells, CulturedABSTRACT
From the alcoholic extract of the lichen Alectoria sarmentosa, four compounds showing antimicrobial activity were isolated. Of these, (-)-usnic acid and physodic acid are well known lichen products, 8'-O-ethyl-beta-alectoronic acid [2] is believed to be an artifact formed during isolation and fractionation, and alectosarmentin [1] is a new natural product whose structure was shown, by spectroscopy and chemical transformations, to be that of a dibenzofuranoid lactol. The antimicrobial activity of these substances accounts for the activity of the lichen.
Subject(s)
Anti-Infective Agents/isolation & purification , Benzofurans/isolation & purification , Lichens/chemistry , Anti-Bacterial Agents , Anti-Infective Agents/chemistry , Anti-Infective Agents/pharmacology , Bacteria/drug effects , Benzofurans/chemistry , Benzofurans/pharmacology , Candida/drug effects , Microbial Sensitivity TestsABSTRACT
Organic solvent extracts of leaves of 4 common edible vegetable plants--Bryophyllum pinnatum, Dialium guincense, Ocimum gratissimum and Vernonia amygdalina--had inhibitory activity for His- to His+ reverse-mutations induced by ethyl methanesulfonate acting on Salmonella typhimurium TA100. The concentrated ethyl acetate, methanol and petroleum ether extracts were heat-stable when dissolved in dimethyl sulfoxide. The Bryophyllum ethyl acetate extract was fractionated into alkaloidal/water-soluble, acids, polar lipid and non-polar lipid fractions. The polar and non-polar lipid fractions inhibited reversion mutations induced by ethyl methanesulfonate acting on TA100 or TA102, and were also active against reversions induced by 4-nitro-O-phenylenediamine and 2-aminofluorene in TA98. The alkaloidal/water-soluble and the acid fractions had no appreciable antimutagenic activities.
Subject(s)
Mutation/drug effects , Plant Extracts/pharmacology , Plants, Edible , Acetates , Alkanes , Dose-Response Relationship, Drug , Ethyl Methanesulfonate/toxicity , Fluorenes/toxicity , Genes, Bacterial/drug effects , Methanol , Mutagenicity Tests , Nigeria , Phenylenediamines/toxicity , Plant Extracts/toxicity , Salmonella typhimurium/geneticsABSTRACT
The dactylocycline antibiotics were found through circular dichroism measurements, NMR spectroscopy and chemical transformations to possess the usual tetracycline family absolute configuration at carbons 4, 4a, 5a and 12a. The absolute stereochemistry about the C-6 carbon, however, was the reverse of that found with previously investigated tetracyclines.
Subject(s)
Actinomycetales/chemistry , Anti-Bacterial Agents/chemistry , Chlortetracycline/analogs & derivatives , Chlortetracycline/chemistry , Circular Dichroism , Magnetic Resonance Spectroscopy , StereoisomerismABSTRACT
Higher plants contain both mutagens and antimutagens and are susceptible to mutagenesis but screening programs for detection of antimutagenesis rarely employ higher plant systems. Short-term bacterial and mammalian tissue culture systems are the norm. Using modified screening tests for detecting antimutagenic agents, higher plants have been shown to contain a variety of structurally novel antimutagenic agents. Systematic bioassay-directed methodology resulted in the isolation in pure form and biological and chemical characterization of the responsible individual active components from various plants. The methodology in use is illustrated by the isolation of cinnamic acid, cinnamyl cinnamate and cinnamyl ricinoleate as the active constituents of the classic medicinal plant product, Styrax asiatica. The methods which may be used to reveal structure-activity relationships and to explore putative molecular modes of action are illustrated with excerpts from the same study.
Subject(s)
Antimutagenic Agents/pharmacology , Plants/chemistry , Antimutagenic Agents/isolation & purification , Genetic Techniques , Structure-Activity RelationshipABSTRACT
Glycyrrhiza glabra polar lipid extract contains a number of flavonoids and related chemical compounds. Studies on the effectiveness of Glycyrrhiza glabra polar lipid extract in intercepting reactive molecules generated from the illumination of the photosensitizers rose bengal and phenosafranin indicate that it is effective in preventing cytotoxicity against E. coli K12+ in a dose-related fashion using illuminated rose bengal. Since only a modest scavenging of singlet oxygen generated from phenosafranin is observed, the effects of the extracts are less related to singlet oxygen-mediated oxidation of substrate (type II reactions) than non-singlet oxygen-mediated oxidation of substrate (type I reactions). Elevated levels of glutathione observed in exponentially growing cells of E. coli K12 were also observed.