ABSTRACT
Dot-ELISA and standard-ELISA were used to detect T. solium cysticercus antigens in cerebrospinal fluid for the diagnosis of neurocysticercosis. The results indicate that both assays are sensitive, specific and economically adequate for the diagnosis of this disease. Using SDS-PAGE, electroblotting and dot-ELISA we have found that the antigen is a protein with a molecular weight of 66,000 daltons.
Subject(s)
Antigens, Helminth/cerebrospinal fluid , Cysticercosis/diagnosis , Taenia/immunology , Adult , Animals , Child , Enzyme-Linked Immunosorbent Assay , HumansABSTRACT
The dot enzyme-linked immunoassay and standard enzyme-linked immunoassay were used to detect Cysticercus cellulosae antigens in cerebrospinal fluid of patients suffering with neurocysticercosis. Using the dot enzyme-linked immunoassay, 10 of 17 patients (59%) were positive at a reciprocal titer of 128 (range 128-1,024). In the standard assay, 13 of 17 (77%) were positive (range 128-512). Specificity was 100% in both assays using 48 cerebrospinal fluids from patients with various central nervous system infections. The quantification of antigens in cerebrospinal fluid using standard assay and photometric readings showed a range of 17 to 138 ng/ml. The results indicate that both assays are sensitive, specific, and economical for the diagnosis of neurocysticercosis.
Subject(s)
Antigens, Helminth/cerebrospinal fluid , Brain Diseases/diagnosis , Cysticercosis/diagnosis , Cysticercus/immunology , Enzyme-Linked Immunosorbent Assay , Taenia/immunology , Adult , Brain Diseases/cerebrospinal fluid , Child , Cysticercosis/cerebrospinal fluid , HumansABSTRACT
Thirteen patients with neurocysticercosis were studied and treated with flubendazole. Diagnostic procedures included computerized tomography (CT) and enzyme-linked immunosorbent assay. In 12 cases treatment with the drug led to clinical improvement. CT showed that some cysts regressed in size, and in two patients they disappeared. The treatment also lowered the antibody levels. The drug was well tolerated and no allergic reaction or other side effects were observed.
Subject(s)
Benzimidazoles/therapeutic use , Brain Diseases/drug therapy , Cysticercosis/drug therapy , Mebendazole/therapeutic use , Adult , Antibodies/analysis , Antibodies/cerebrospinal fluid , Brain/diagnostic imaging , Brain/parasitology , Brain Diseases/etiology , Brain Diseases/parasitology , Child , Cysticercosis/immunology , Cysticercosis/parasitology , Cysticercus/immunology , Female , Humans , Male , Mebendazole/analogs & derivatives , Middle Aged , Tomography, X-Ray ComputedABSTRACT
La frecuencia elevada de cisticersosis en paises en desarrollo y sus efectos patologicos y economicos senalan la necesidad de contar con una prueba satisfactoria para diagnosticar esta enfermedad. En el Hospital Central Ignacio Morones Prieto de San Luis Potosi, SLP, Mexico, se realizo un estudio en el que se comparo la sensibilidad del ensayo inmunosorbente enzima conjugada (ELISA) con la del metodo de hermaglutinacion indirecta (HI), para diagnosticar la cisticercosis. Por medio de ambos metodos se analizaron muestras de liquido cefalorraquideo y de suero de 120 pacientes neurologicos que se dividieron en dos grupos control y un grupo de 17 casos de cisticercosis cerebral confirmada. Las muestras de los grupos control resultaron negativas con ambos procedimientos, pero en el grupo de enfermos de cisticercosis con el metodo de HI se obtuvo 29% de resultados falsos negativos, en tanto que con ELISA no se obtuvo ninguno.Se concluye que el metodo ELISA es mas sensible que el de HI puesto que con el no se lograron resultados falsos negativos ni positivos sin embargo, en vista del pequeno numero de casos investigados no es posible llegar a una conclusion definitiva
Subject(s)
Humans , Cysticercosis , Brain Diseases , Enzyme-Linked Immunosorbent Assay , Hemagglutination Tests , Cerebrospinal FluidABSTRACT
The effect of flubendazole on Cysticercus cellulosae was tested in 15 pigs. In doses o 8.3 mg/kg body weight or more daily for 10 days the drug was lethal to the cysticerci, causing both macroscopic and microscopic morphological alterations. An in vitro test showed 0% viability of cysticerci in most of the treated pigs, whereas in 90-100% of 11 control pigs they were viable.
