ABSTRACT
The uptake of amino acids and glucose by BHK 21 clone 13 suspension cells grown in Modified Eagles Medium was monitored. The Growth Yields for sixteen amino acids and glucose were determined. Four "non essential" amino acids were utilised. Glutamine, glutamate, leucine and asparagine were used in greatest quantity by the cells which also utilised virtually all the cystine in the medium. The implications of these results are discussed.
Subject(s)
Amino Acids/metabolism , Glucose/metabolism , Animals , Biological Transport , Cell Division , Cell Line , Clone Cells , Cricetinae , Kidney , KineticsSubject(s)
Cell Line/metabolism , Oxygen Consumption , Animals , Cell Division , Cricetinae , KidneyABSTRACT
A medium is described which will support the submerged culture of BHK-21 cells to 6.5 x 10(6) to 7 x 10(6) cells/ml in volumes up to 30 liters. Shaken-flask cultures were used to determine nutrient deficiencies in depleted medium. The final medium became limited by glutamine at 6.5 x 10(6) to 7 x 10(6) cells/ml, but increasing the glutamine concentration failed to improve cell yields. The value of the polyol Pluronic F68 as a protective substance is illustrated.
