ABSTRACT
The biological significance of growth hormone (GH) in the physiology and pathophysiology of the immune system is not established. To address the site and mode of action through which GH exerts its effects on lymphocyte tumors, we applied a well-characterized monoclonal antibody directed against the hormone binding site of the receptor and were able to further characterize the tumor by immunohistochemical localization of GH receptors. Cutaneous T cell lymphomas were identified by histologic and immunomorphologic diagnosis according to the updated Kiel classification, with the application of monoclonal antibodies. Nodular tumors of the skin, identified as highly malignant Ki-1 lymphomas of large anaplastic cells, had intense GH receptor immunoreactivity. The presence of GH receptors in these proliferating tumor cells supports the hypothesis that GH is involved in paracrine-autocrine mechanisms acting locally in regulating peripheral T cell lymphoma tumor growth.
Subject(s)
Lymphoma, Large-Cell, Anaplastic/chemistry , Receptors, Somatotropin/analysis , Skin Neoplasms/chemistry , Antibodies, Monoclonal , Humans , Immunohistochemistry , Immunophenotyping , Lymphoma, Large-Cell, Anaplastic/immunology , Lymphoma, Large-Cell, Anaplastic/pathology , Skin Neoplasms/immunology , Skin Neoplasms/pathologyABSTRACT
We used several biotinylated neoglycoproteins as tumor markers to detect and localize endogenous carbohydrate-binding proteins in cultured hepatoblastoma, melanoma, and bladder carcinoma tumor cells. The neoglycoproteins used consisted of cellobiose, fucose, N-acetyl-galactosamine, N-acetyl-glucosamine, lactose, maltose, mannose, melibiose, and xylose. In addition, naturally occurring asialofetuin that was chemically disialylated was also used. Binding to the cultured tumor cells was made visible with the avidin-peroxidase technique. Depending on the type of neoglycoprotein used, markedly different expression of cytoplasmic and nuclear receptors for sugars (endogenous lectins) was obtained from rat hepatoblastoma, human melanoma, and bladder carcinoma tumor cells. The most pronounced staining differences were documented for asialofetuin and the neoglycoproteins containing fucose, N-acetyl-galactosamine, and lactose.
