ABSTRACT
The increasing popularity of the concept of liquid biopsy, which involves the definition of circulating tumor cells and circulating nucleic acids in the blood can significantly improve long-term results of treatment for gastric cancer due to the high sensitivity of techniques. It needs further study in relation to gastric cancer, the search for universal markers and methods.
Subject(s)
Biomarkers, Tumor/blood , Circulating Tumor DNA/blood , Liquid Biopsy , Stomach Neoplasms/blood , Humans , MicroRNAs/blood , Neoplastic Cells, Circulating/metabolism , Neoplastic Cells, Circulating/pathology , Stomach Neoplasms/pathologyABSTRACT
Standard methods of diagnostics haven't got a proper sensitivity and specificity concerning assessment of regional nodal involvement in case of rectal cancer. Therefore it is necessary to look for new method of diagnostics, detect risk factors and unfavorable prognosis in relation to lateral lymph node metastasis. At the same time, there should be a differentiated approach to the choice of therapeutic management in rectal cancer. Investigation of variability of blood supply of the rectum could be an additional method of diagnostics in rectal cancer.
Subject(s)
Lymph Nodes/pathology , Rectal Neoplasms/secondary , Risk Assessment/methods , Humans , Lymphatic Metastasis/diagnosis , Pelvis , Prognosis , Rectal Neoplasms/diagnosis , Risk FactorsABSTRACT
Copies of two repetitive elements of the genome of common tree shrew (Tupaia glis) were cloned and sequenced. The first element, Tu III, is a approximately 260 bp long short interspersed element (SINE) with the 5'-end derived from glycine RNA. Tu III carries long polypurine- and polypyrimidine-rich tracts, which may contribute to the specific secondary structure of Tu III RNA. This SINE was also found in the genome of smooth-tailed tree shrew of another genus (Dendrogale). Tu III seems to be confined to the order Scandentia (tree shrews) since it was not found in DNA of other tested mammals. The second element Tu-SAT1 is a tandem repeat with a monomer length of 365 bp. Some properties of its nucleotide sequence suggest that Tu-SAT1 is a centromeric satellite.
