[Effects of quercetin on morphology and VEGF secretion of leukemia cells NB4 in vitro].

OBJECTIVE: To investigate the effects of quercetin on cell morphology and VEGF expression of acute myeloblastic leukemia cells NB4 in vitro. METHODS: The cytomorphology of NB4 cells was assessed by Wright-stain, apoptosis rate by apoptotic marker Annexin V, and VEGF secretion level by ELISA. RESULTS: Typical apoptosis was found in NB4 cells after treatment with quercetin. Apoptotic marker Annexin V analysis showed that the apoptotic rate of NB4 cells was increased after treatment with quercetin. The secretion of VEGF of NB4 cells was significantly decreased after treatment with quercetin. CONCLUSION: Quercetin can induce apoptosis and inhibit secretion of VEGF in NB4 leukemia cells.