Unmodified Clay Nanosheets at the Air-Water Interface.

Quasi-two-dimensional (2D) nanolayers, such as graphene oxide or clay layers, adhere to gas-liquid or liquid-liquid interfaces. Particularly, clays are of wide general interest in this context because of their extensive and crucial use as Pickering emulsion stabilizers, as well as for their ability to provide colloidosome capsules. So far, clays could only be localized at oil-water or air-saline-water interfaces in aggregated states, while our results now show that clay nanosheets without any modification can be located at air-deionized-water interfaces. The clay mineral used in the present work is synthetic fluorohectorite with a very high aspect ratio and superior quality in homogeneity and charge distribution compared to other clay minerals. This clay mineral is more suitable for achieving unmodified clay anchoring to fluid interfaces compared to other clay minerals used in previous works. In this context, we studied clay nanosheet organization at the air-water interface by combining different experimental methods: Langmuir-Blodgett trough studies, scanning electron microscopy (SEM) studies of film deposits, grazing-incidence X-ray off-specular scattering (GIXOS), and Brewster angle microscopy (BAM). Clay films formed at the air-water interface could be transferred to solid substrates by the Langmuir-Schaefer method. The BAM results indicate a dynamic equilibrium between clay sheets on the interface and in the subphase. Because of this dynamic equilibrium, the Langmuir monolayer surface pressure does not change significantly when pure clay sheets are spread on the liquid surface. However, also, GIXOS results confirm that there are clay nanosheets at the air-water interface. In addition, we find that clay sheets modified by a branched polymer are much more likely to be confined to the interface.

Diffusion maps of Bacillus subtilis biofilms via magnetic resonance imaging highlight a complex network of channels.

Bacillus subtilis microorganism when cultivated under chemically-defined conditions developed a biofilm with an unusual pattern of wrinkles on the surface. Some questions were raised about whether there was a special function of these wrinkles for the biofilm itself, since they resembled microchannels that could be involved in the transport of nutrients within the biofilm. Since the diffusion is the main mechanism for nutrient transport to biofilm from the medium, the role of these wrinkled structures in the whole diffusion within the biofilm was investigated using diffusion-weighted magnetic resonance imaging (DW-MRI). Data from these diffusion images was used to generate 2D diffusion maps which highlighted the striking channel features of the biofilm surface. The diffusion maps revealed a network of interconnected channels, with self-diffusion coefficients higher in the microchannels than in other regions of the biofilms. Polar plots made from 2D diffusion maps obtained from the plane of the biofilm show an anisotropy of the diffusion inside the microchannels, with the diffusion higher when along the principal direction of the microchannels. These results agree with the model, that the buckling of the biofilm surface from the B. subtilis creates microchannels that can enhance diffusion throughout the biofilm.

Patterns in Saccharomyces cerevisiae yeast colonies via magnetic resonance imaging.

We report the use of high-resolution magnetic resonance imaging methods to observe pattern formation in colonies of Saccharomyces cerevisiae. Our results indicate substantial signal loss localized in specific regions of the colony rendering useful imaging contrast. This imaging contrast is recognizable as being due to discontinuities in magnetic susceptibility (χ) between different spatial regions. At the microscopic pixel level, the local variations in the magnetic susceptibility (Δχ) induce a loss in the NMR signal, which was quantified via T2 and T2* maps, permitting estimation of Δχ values for different regions of the colony. Interestingly the typical petal/wrinkling patterns present in the colony have a high degree of correlation with the estimated susceptibility distribution. We conclude that the presence of magnetic susceptibility inclusions, together with their spatial arrangement within the colony, may be a potential cause of the susceptibility distribution and therefore the contrast observed on the images.

Intercalated water in synthetic fluorhectorite clay.

(7)Li and (1)H nuclear magnetic resonance together with X-ray diffraction measurements in powdered samples and pseudocrystalline films of synthetic fluorhectorite as a function of relative ambient humidity permit to address several aspects of the structure and dynamics of intercalated water molecules. The role of proton exchange as a possibly dominant mechanism of charge transport in the one-water layer regime of hydration is reexamined. The experimental results in Li-fluorhectorite support the result of molecular simulations which predict, for Li-montmorillonite, the existence of an intermediate regime, between one-water layer and two-water layer states.

Synthesis of thiosemicarbazone and 4-thiazolidinone derivatives and their in vitro anti-Toxoplasma gondii activity.

Thiosemicarbazone and 4-thiazolidinone derivatives were synthesized in one and two step, respectively, from thiosemicarbazide, in satisfactory yields. Then, the synthesized compounds were submitted to evaluation against host cells infected with Toxoplasma gondii. The present studies showed that thiosemicarbazones 2 and 4-thiazolidinone derivatives 3 were effective against intracellular T. gondii.