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1.
Rev Sci Instrum ; 88(12): 123301, 2017 Dec.
Article in English | MEDLINE | ID: mdl-29289172

ABSTRACT

We demonstrate a capability of deterministic doping at the single atom level using a combination of direct write focused ion beam and solid-state ion detectors. The focused ion beam system can position a single ion to within 35 nm of a targeted location and the detection system is sensitive to single low energy heavy ions. This platform can be used to deterministically fabricate single atom devices in materials where the nanostructure and ion detectors can be integrated, including donor-based qubits in Si and color centers in diamond.

2.
J Comp Neurol ; 303(3): 424-34, 1991 Jan 15.
Article in English | MEDLINE | ID: mdl-2007658

ABSTRACT

A population of cells has been found in the chick optic tract and chiasm exhibiting GABA-like immunoreactivity (GABA+; Granda and Crossland, J. Comp. Neurol. 287:455-469, '89). It is not known, however, whether the cells are neurons. We have studied the GABA+ cells by using morphological and immunocytochemical methods. We found that there are more than 500 cells in each tract. At the light microscopic level, the cells possess processes resembling dendrites and axons. At the electron microscopic level, the organelle content of the cells is similar to that of neurons. The cells are immunoreactive with antibodies to MAP2 and neuron specific enolase, two proteins characteristic of neurons. Taken together the findings indicate that the GABA+ cells of the chick optic tract are neurons, perhaps similar to the interstitial neurons found in the white matter of other vertebrates.


Subject(s)
Chickens/anatomy & histology , Optic Chiasm/cytology , Optic Nerve/cytology , gamma-Aminobutyric Acid/analysis , Animals , Antibodies, Monoclonal/immunology , Eye Enucleation , Microscopy, Electron , Microtubule-Associated Proteins/analysis , Microtubule-Associated Proteins/immunology , Neurons/chemistry , Neurons/ultrastructure , Optic Chiasm/chemistry , Optic Nerve/chemistry , Organelles/ultrastructure , Visual Pathways/anatomy & histology
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