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1.
Nat Commun ; 14(1): 2537, 2023 05 03.
Article in English | MEDLINE | ID: mdl-37137944

ABSTRACT

The genomes of most protozoa encode families of variant surface antigens. In some parasitic microorganisms, it has been demonstrated that mutually exclusive changes in the expression of these antigens allow parasites to evade the host's immune response. It is widely assumed that antigenic variation in protozoan parasites is accomplished by the spontaneous appearance within the population of cells expressing antigenic variants that escape antibody-mediated cytotoxicity. Here we show, both in vitro and in animal infections, that antibodies to Variant-specific Surface Proteins (VSPs) of the intestinal parasite Giardia lamblia are not cytotoxic, inducing instead VSP clustering into liquid-ordered phase membrane microdomains that trigger a massive release of microvesicles carrying the original VSP and switch in expression to different VSPs by a calcium-dependent mechanism. This novel mechanism of surface antigen clearance throughout its release into microvesicles coupled to the stochastic induction of new phenotypic variants not only changes current paradigms of antigenic switching but also provides a new framework for understanding the course of protozoan infections as a host/parasite adaptive process.


Subject(s)
Giardia lamblia , Giardiasis , Intestinal Diseases, Parasitic , Parasites , Animals , Giardia lamblia/genetics , Giardia lamblia/metabolism , Parasites/metabolism , Antigens, Surface/genetics , Antigens, Surface/metabolism , Antigens, Protozoan , Antibodies/metabolism , Antigenic Variation , Protozoan Proteins/genetics , Protozoan Proteins/metabolism
2.
Nat Commun ; 10(1): 361, 2019 01 21.
Article in English | MEDLINE | ID: mdl-30664644

ABSTRACT

Intestinal and free-living protozoa, such as Giardia lamblia, express a dense coat of variant-specific surface proteins (VSPs) on trophozoites that protects the parasite inside the host's intestine. Here we show that VSPs not only are resistant to proteolytic digestion and extreme pH and temperatures but also stimulate host innate immune responses in a TLR-4 dependent manner. We show that these properties can be exploited to both protect and adjuvant vaccine antigens for oral administration. Chimeric Virus-like Particles (VLPs) decorated with VSPs and expressing model surface antigens, such as influenza virus hemagglutinin (HA) and neuraminidase (NA), are protected from degradation and activate antigen presenting cells in vitro. Orally administered VSP-pseudotyped VLPs, but not plain VLPs, generate robust immune responses that protect mice from influenza infection and HA-expressing tumors. This versatile vaccine platform has the attributes to meet the ultimate challenge of generating safe, stable and efficient oral vaccines.


Subject(s)
Giardia lamblia/chemistry , Influenza Vaccines/immunology , Membrane Proteins/immunology , Orthomyxoviridae Infections/prevention & control , Protozoan Proteins/immunology , Vaccines, Virus-Like Particle/immunology , Adjuvants, Immunologic , Administration, Oral , Animals , Antigen Presentation/drug effects , Bioengineering/methods , Dendritic Cells/drug effects , Dendritic Cells/immunology , Dendritic Cells/virology , Female , Gene Expression , Hemagglutinin Glycoproteins, Influenza Virus/genetics , Hemagglutinin Glycoproteins, Influenza Virus/immunology , Humans , Immunity, Innate/drug effects , Influenza Vaccines/administration & dosage , Influenza Vaccines/genetics , Male , Membrane Proteins/genetics , Mice , Mice, Transgenic , Neuraminidase/genetics , Neuraminidase/immunology , Orthomyxoviridae Infections/immunology , Orthomyxoviridae Infections/virology , Protein Stability , Protozoan Proteins/genetics , Toll-Like Receptor 4/genetics , Toll-Like Receptor 4/immunology , Trophozoites/chemistry , Vaccination , Vaccines, Virus-Like Particle/administration & dosage , Vaccines, Virus-Like Particle/genetics
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