ABSTRACT
BACKGROUND: This study aimed to describe the clinical manifestations and surgical outcomes of subluxated lens. METHODS: Reviewing charts from January 1991 to June 2000, we studied 110 patients (123 eyes) who had lens subluxation at Chang Gung Memorial Hospital, Kaohsiung. For 21 eyes, patients accepted conservative treatment, and 102 eyes underwent surgery (8 eyes had Marfan syndrome; 18 eyes had idiopathic dislocation; and 76 eyes had experienced trauma). Indications of surgery included reduction in visual acuity, uncorrectable refractive error, and secondary glaucoma. RESULTS: There were 81 male and 29 female patients. Their mean age was 47.6 years (range, 2-76 years). The mean post-operative follow-up period was 22.24 months (range, 2-118 months). In spite of the different lens statuses, similar visual results were found between the surgical treatment group and the nonsurgery group. In the surgery group, 47 eyes (46.1%) achieved best corrected visual acuity (BCVA) of 20/40 or better. A total of 80 eyes (78.4%) achieved an improvement (log MAR change >0.3) in BCVA. The major postoperative complications included glaucoma (11 eyes), retinal detachment (9 eyes) and intraocular lens dislocation into the vitreous cavity (3 eyes). CONCLUSIONS: Results indicate that the presence of a subluxated lens alone is not an absolute indication for surgical removal. The lens status had no significant influence on surgical outcome. Most cases can achieve an improvement in BCVA after appropriate management.
Subject(s)
Lens Subluxation/surgery , Adolescent , Adult , Aged , Child , Child, Preschool , Female , Humans , Intraocular Pressure , Lens Subluxation/physiopathology , Male , Middle Aged , Postoperative Complications , Visual AcuityABSTRACT
This study was aimed at investigating the impact of antibiotic pulse treatment (APT) of penicillin-G and streptomycin sulfate at a concentration of 1000 mg/l each on the regeneration ability of leaf and shoot-base explants and homogenized sporophytes of Platycerium bifurcatum. The reaction of the plant to APT, as measured by changes in sporophyte regeneration and morphology, was considered to be a stress response. Cell's susceptibility to APT was graded as: tissue homogenized prior to treatment>tissue homogenized after treatment>leaf and shoot-base explants. Biphasic inhibition in sporophyte regeneration was observed in homogenized sporophytes; a sharper decrease occurred with 20-60 min of the APT, followed by a slower decline for up to 180 min. No significant impact on the regeneration ability of leaf and shoot-base explants was found. Morphologically, APT caused leaf cleavage, mesophyll damage and hyperhydricity in some sporophytes. Except for the hyperhydric sporophytes, regenerants were able to continue their growth in soil, and no carry-over effect was found in the next generation.
ABSTRACT
A simple suspension culture system of Platycerium bifurcatum was developed where sporophytes could be regenerated directly from leaf cells or indirectly through an aposporous gametophyte stage under the same culture conditions. Single cells and aggregates of up to 100 cells developed aposporous gametophytes which later gave rise to sporophytes. Such gametophytes started apogamy when they were mostly less than 0.7 mm in length, bearing only rhizoids. In most cases, only one sporophyte was regenerated from one gametophyte. Aggregates of 500-1000 or more cells, on the other hand, regenerated sporophytes directly. Intercellular interaction was considered to be the physiological cause, and the separation of leaf cells to a certain degree drove the cells to embark on different regeneration paths. It is suggested that the possible existence of a threshold size of cell aggregates separates the two regeneration patterns.
ABSTRACT
A rapid and reliable micropropagation method was established for Spathoglottis plicata. Nodal and leaf explants dissected from 8-month-old pot-grown seedlings were cultured on charcoal-amended Murashige and Skoog medium supplemented with 16 combinations of α-naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BA) at concentrations of 0.54-10.74 µM. Regeneration of protocorm-like bodies (PLBs) and subsequent plantlet development were observed from 98.5% of the nodal explants. Only 6.5% of leaf explants and occasionally some root segments (dissected from regenerated plantlets) were able to produce PLBs and then plantlets. The optimum plant growth regulator (PGR) combination for maximal PLB regeneration was 5.37 µM NAA and 0.44 µM BA. The best combination of PGR for plantlet development was 2.69-10.74 µM NAA and 8.88 µM BA. The NAA to BA ratios for maximal PLB induction and plantlet development were 12.2 and 0.3-1.2, respectively. Regenerated PLBs and plantlets, when cut into pieces of less than 1 mm and subcultured onto the above media, regenerated new PLBs and plantlets in another 3 months.
