ABSTRACT
Synopsis Hair damage, caused by sunlight, and the possibility to protect hair against sunlight by means of cosmetic formulations was studied. For this, five UV-filters were tested: benzophenone-3 (UV-A/UV-B); benzophenone-4 (UV-A/UV-B); phenylbenzimidazole sulfonic acid (UV-B); butylmethoxydibenzoylmethane (UV-A); octyl dimethyl PABA (UV-B). The stability of the UV-filters was tested as pure substances as well as in two cosmetic formulations: a setting lotion and a shine spray. The degree of decolouration and stress strain behaviour were determined. The benzophenones had the best protection ability both on colour and on morphology. Stability data and UV-spectra are discussed.
ABSTRACT
The nucleotide sequence of a chloramphenicol-resistance (CmR) determinant from the Gram- soil bacterium Agrobacterium tumefaciens was determined, and its gene product was identified as Cm acetyltransferase (CAT). Comparison of the amino acid sequences of the A. tumefaciens CAT and various CAT proteins of Gram+ and Gram- origin shows no homology between this and the other enzymes.
Subject(s)
Chloramphenicol O-Acetyltransferase/genetics , Chloramphenicol Resistance/genetics , Rhizobium/genetics , Amino Acid Sequence , Base Sequence , Chloramphenicol O-Acetyltransferase/metabolism , Chromatography, Thin Layer , DNA, Bacterial , Molecular Sequence Data , Restriction Mapping , Rhizobium/drug effects , Rhizobium/enzymology , Sequence Homology, Nucleic AcidABSTRACT
Nitrogen-fixingAnabaena cylindrica cells are found to evolve hydrogen in high quantities in the presence of CO plus C2H2. Studies with the inhibitors dichlorophenyldimethylurea (DCMU), disalicylidenepropanediamine (DSPD), dibromothymoquinone (DBMIB), undecylbenzimidazole (UDB) and chloro-carbonyl-cyanide-phenylhydrazone (CCCP) and also withAnabaena grown on nitrate- and ammonia-nitrogen show that the H2-formation is due to the ATP-dependent H3O(+)-reduction catalysed by nitrogenase. In control experiments CO plus C2H2 inhibited the activities of a cell-free hydrogenase fromClostridium pasteurianum. It is concluded that Anabaena has a hydrogenase whose natural function is to recycle the H2 lost by the action of nitrogenase.
