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Reprod Domest Anim ; 56(11): 1464-1469, 2021 Nov.
Article in English | MEDLINE | ID: mdl-34339545

ABSTRACT

This study aimed to describe successful cryopreservation of sperm from maned wolves (Chrysocyon brachyurus). Three ejaculates from 2 maned wolves were collected by digital manipulation of the penis and evaluated subjectively, centrifuged and frozen in BotuCrio® (Botupharma, Botucatu, Brazil) or Tris-yolk egg extender. Spermatozoa were thawed at 37ºC/30s or 70ºC/4s and evaluated for kinetics, morphology, plasma and acrosome membrane integrity, mitochondrial potential, hydrogen peroxide, superoxide anion and lipid peroxidation. From 5 thawed samples, two had sperm total motility >55% (56.0% and 64.0%) and progressive motility ~35% (35% and 40%), both frozen with Tris-yolk egg. Plasma and acrosome membrane integrity decreased and percentage of sperm defects increased post-thawing. We concluded that is possible to freeze spermatozoa from maned wolves using semen collection and processing methods applied for domestic dogs.


Subject(s)
Canidae/physiology , Cryopreservation/veterinary , Semen Preservation/veterinary , Animals , Cryopreservation/methods , Freezing , Male , Semen Preservation/methods , Sperm Motility , Spermatozoa/cytology , Spermatozoa/physiology
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