Subject(s)
Esophagitis, Peptic/physiopathology , Gastrointestinal Hormones/physiology , Hydrocortisone/physiology , Neurotransmitter Agents/physiology , Pancreatic Hormones/physiology , Peptic Ulcer/physiopathology , Duodenum/innervation , Duodenum/physiopathology , Esophagitis, Peptic/complications , Esophagus/innervation , Esophagus/physiopathology , Humans , Peptic Ulcer/complications , Stomach/innervation , Stomach/physiopathology , Vagus Nerve/physiopathologyABSTRACT
Studies of the extracting ability of hexane-isopropanol (2:1) and chloroform-methanol in respect of lipid substrates, their peroxidation products and antioxidative activity have demonstrated the advantages of using the hexane lipid phase.
Subject(s)
Lipid Peroxidation , Lipids/isolation & purification , Animals , Dogs , Free Radicals , HumansABSTRACT
Experimentally on 116 dogs it has been found that intravascular shunts of a big diameter or those controlled by means of extracorporal shunts ensure stable hemodynamic indices, approximately the initial ones. The isolated hypothermal perfusion protects, in its turn, the liver from an ischemic trauma and prolongs the safety period of the liver blood flow break to 40 minutes and more.
Subject(s)
Arteriovenous Shunt, Surgical , Liver/surgery , Animals , Dogs , Female , Hepatic Artery/surgery , Hypothermia, Induced , Ligation , Liver/blood supply , Liver Circulation , Male , Perfusion , Portal Vein/surgery , Vena Cava, Inferior/surgeryABSTRACT
The effect of cooling and subsequent rewarming on the tissue respiration of canine hearts was studied during polycomponent ether-oxygen anaesthesia. The tests included the determinations of the activity of the dehydrogenases of the cytrate cycle, the content and activity of chromoproteids, the respiration rate of the mitochondrias on succinate, glutamate and ketoglutarate, the content of glycogen, the activity of the phosphorylases, hexokinase, lactate dehydrogenase, the content of lactate, pyruvate, adenyl nucleotides and creatine phosphate. Significant changes were noted in the content and activity of the above substances, acceleration of mitochondrial respiration, reduced energy regulation of respiration, and decreased amount of the adenyl components. It is suggested that under artificial hypothermia the processes of chromoproteids biosynthesis are enhanced, which results in an increased power of terminal respiration, and conformational rearaangements of the enzymes connected with the membranes occur.
Subject(s)
Energy Metabolism , Hypothermia, Induced , Myocardium/metabolism , Adenosine Triphosphatases/metabolism , Animals , Carbohydrate Metabolism , Catalase/metabolism , Cytochrome c Group/metabolism , Dogs , Electron Transport Complex IV/metabolism , Ketoglutarate Dehydrogenase Complex/metabolism , Malate Dehydrogenase/metabolism , Male , Myoglobin/metabolism , Oxidative Phosphorylation , Oxygen Consumption , Succinate Dehydrogenase/metabolismSubject(s)
Extracorporeal Circulation , Liver/metabolism , Animals , Dogs , Ketoglutarate Dehydrogenase Complex/metabolism , L-Lactate Dehydrogenase/metabolism , Lactates/metabolism , Liver/enzymology , Liver Glycogen/biosynthesis , Malate Dehydrogenase/metabolism , Male , Mitochondria, Liver/metabolism , Oxidation-Reduction , Succinate Dehydrogenase/metabolismABSTRACT
The article deals with oxidation of different substrates, intensity of glycolytic and glycogenolytic processes in mitochondria and homogenates of dog liver with its 2-hour exclusion from circulation under conditions of endotracheal ether-oxygen narcosis. It was established that already 30-60-minute ischemia causes a decrease in intensity of succinate, alpha-ketoglutarate oxidation and acceptor respiration, inhibiton in the activity of the citrate cycle enzymes; succinate dehydrogenase, alpha-ketoglutarate dehydrogenase, isocytrate dehydrogenase. The activity of NAD-dependent malate dehydrogenasedehydrogenase and Mg2+-ATPase as well as intensity of NADN oxidation in mitochondria increase. After 2-hour ischemia the activity of Mg2+-ATPase, cytochrome oxidase and peroxidase lowers. A sharply developed glycogenolysis is accompanied by inhibition of phosphorylase activity and a two-fold stimulation of the glycolytic reactions. Peculiarities in regulation of enzymatic reactions under conditions of ischemia and their role in origin of metabolism disturbances in the liver are under discussion.