ABSTRACT
The role of biologically active substances in ultra-low doses (10(-15)-10(-27) mol/l) is discussed from the different points of view. The most detailed analysis of neurobiological effects produced by these doses can be studied on the preparate of completely isolated molluscan neurones. In this case the possibility arises to control the first modifications of action at the electrophysiological characteristics of neuronal activity. These changes of electrical activity can be regarded as a reaction to biologically active substance. The following characteristics were controlled: the level of membrane resting potential (MP), the electroexcitable membrane and pacemaker mechanism, chemical sensitivity of somatic membrane loci to neurotransmitter acetylcholine (Ach). Several substances were used in these experiments: two kinds of synthetic antioxidant, GABA, ethanol, serotonine, DSIP (delta-sleep inducing peptide), antibiotic ruboxil, nootrop GVS-111. The isolated neurones were placed into the special chamber. All these substances (0.35 ml) were added single dosing into this chamber with living physiological solution in concentration 10(-15)-10(-27) mol/l. The results demonstrated that all substances had initiated the development of prolonged neurophysiological responses. The intensities of neuronal reactions didn't depend in contact period on the concentration and on the type of substance. It is suggested that these data reveal the existence of unknown modes of regulation of neuronal functional states and presence of hidden channel for information transfer and receiving. This different way of regulation is extremely important influence living organisms.
Subject(s)
Neurons/drug effects , Neurons/physiology , Neurotransmitter Agents/pharmacology , Acetylcholine/pharmacology , Animals , Anti-Bacterial Agents/pharmacology , Antioxidants/pharmacology , Cell Membrane/drug effects , Delta Sleep-Inducing Peptide/pharmacology , Dipeptides/pharmacology , Dose-Response Relationship, Drug , Electrophysiology/methods , Ethanol/pharmacology , Helix, Snails , In Vitro Techniques , Linear Models , Membrane Potentials/drug effects , Serotonin/pharmacology , Time Factors , gamma-Aminobutyric Acid/pharmacologyABSTRACT
The influence of inhibitors of radical lipid oxidation on completely isolated identified neurons LPa3 was investigated. The correlation was found between the concentration of acting inhibitor and the level of electrical activity alteration of the neuron. The maximum effect was observed at the inhibitor concentration of 10(-15) M.
Subject(s)
Antioxidants/pharmacology , Neurons/physiology , Animals , Electric Stimulation , Free Radicals , Helix, Snails , In Vitro Techniques , Lipid Metabolism , Membrane Potentials/drug effects , Neurons/drug effects , Oxidation-Reduction , Picolines/pharmacologyABSTRACT
Alterations in concentration of free fatty acids, free cholesterol, native antioxidants as well as in the antioxidative activity were studied in lipids of mice liver tissue and small intestinal mucosa. The intensity of free radical reactions in lipids of animal tissues was affected directly by administration of synthetic inhibitors of the reactions. The inverse correlation was observed between the alteration in concentrations of native antioxidants and free fatty acids as well as between the antioxidative activity of lipids and amount of free cholesterol in them. Free fatty acids appears to be the constant participants in the system of free radical oxidation of lipids, while cholesterol can center the system under distinct level of these reactions intensity.
Subject(s)
Cholesterol/metabolism , Fatty Acids, Nonesterified/metabolism , Intestine, Small/metabolism , Lipid Metabolism , Liver/metabolism , Animals , Circadian Rhythm , Free Radicals , Intestinal Mucosa/metabolism , Male , Mice , Mice, Inbred BALB C , Oxidation-ReductionABSTRACT
It has been shown on the model of thermic oxidation of oleic acid methyl ether that addition of phosphatidylethanolamine and lisophosphatidylcholine into a developed reaction brings about some regularities which can be accounted for as oxidation of two-substances. It is suggested that phospholipids can be the substrate of radical oxidation of substances in oxidative transformations of lipids in the cell. In this case according to oxidation conditions the same phospholipids can appear either as prooxidants or as antioxidants.
Subject(s)
Lysophosphatidylcholines , Oleic Acids , Phosphatidylethanolamines , Oxidation-ReductionABSTRACT
An inverse correlation was observed between the alterations in concentration of free cholesterol and an antioxidative activity of lipids in several tissues and cells. The effect was studied in mice liver tissue on reparative regeneration after partial hepatectomy, in intact mice liver under the directed action on the system of free radical reactions of cell lipid oxidation and in a culture of Hela cells during various periods of cell cycle. Using as a model system the thermal oxidation of oleic acid methyl ester cholesterol was found to function as a substrate in the reactions of free-radical cooxidation and the products of oxidation of cholesterol were active as antioxidants. The significance of a system containing cholesterol and its oxidizing products is discussed in connection with the metabolism of cholesterol itself and in reactions, related to alteration in free-radical oxidation of cell lipids.
