ABSTRACT
We studied the role of hydrogen peroxide in the response of tumor cells to treatment with ozonated culture medium. Changes of the level of hydrogen peroxide in tumor cells incubated in ozonated medium were detected by using fluorescence microscopy and genetically-encoded sensor HyPer2. Modifications of fluorescent properties of the sensor reflecting accumulation of hydrogen peroxide in the cell cytoplasm were detected within 70 min from the start of exposure. The concentration of hydrogen peroxide continued to increase until 375 min. The revealed changes support the involvement of hydrogen peroxide in the cell response to ozone treatment.
Subject(s)
Hydrogen Peroxide/pharmacology , Ozone/chemistry , Culture Media , HeLa Cells , Humans , Microscopy, Confocal , Microscopy, FluorescenceSubject(s)
Mucositis , Pharynx , Stomatitis , Antineoplastic Agents/adverse effects , Humans , Mucositis/diagnosis , Mucositis/etiology , Mucositis/prevention & control , Mucositis/therapy , Pharynx/drug effects , Pharynx/radiation effects , Radiotherapy/adverse effects , Severity of Illness Index , Stomatitis/diagnosis , Stomatitis/etiology , Stomatitis/prevention & control , Stomatitis/therapyABSTRACT
The authors studied the results of preoperative diagnosis of tumors of the small intestine and the late-term results of operative treatment of malignant new growth in 25 patients. It is shown that the X-ray method allows tumors to be detected in some cases but its possibilities are limited. Signs of ileus prevail in the clinical picture in malignant tumors, while signs of intestinal bleeding prevail in benign tumors. Resection of the intestine with the tumor and regional lymph nodes in sarcomas produces satisfactory results in some cases.
Subject(s)
Ileal Neoplasms/surgery , Ileocecal Valve , Jejunal Neoplasms/surgery , Female , Gastrointestinal Hemorrhage/diagnosis , Gastrointestinal Hemorrhage/etiology , Gastrointestinal Hemorrhage/surgery , Humans , Ileal Neoplasms/complications , Ileal Neoplasms/diagnosis , Intestinal Obstruction/diagnosis , Intestinal Obstruction/etiology , Intestinal Obstruction/surgery , Jejunal Neoplasms/complications , Jejunal Neoplasms/diagnosis , MaleABSTRACT
The article deals with the results of prehospital appraisal of hyperplasia of the mammary glands in 364 females by means of infrared thermovision techniques and UHF-radiothermometry. The thermovision and radiothermometric signs of this pathological condition are described. Examination conducted in the course of the disease twice in 52% and 3 to 4 times in 30% of cases showed radiothermometry to be highly informative in optimization of the management of patients with mastopathy. The feature of the thermal metabolic process in cancer was revealed. The proposed radiothermometric method may be used as an auxiliary criterion for appraising the condition of the mammary tissues.
