ABSTRACT
Between 24 March and 31 July 2009, 342 clinically diagnosed cases of rubella were notified in five municipalities in Republika Srpska, Bosnia and Herzegovina. Fourteen cases were laboratory-confirmed by positive IgG against rubella virus. Four virus isolates were obtained and identified as genotype 2B strains, with one isolate differing by a single mutation in the region of the E1 gene. This ongoing outbreak revealed gaps in the immunisation programme during the war in BiH (1992-1995) and highlights the need to revise legislation to permit immunisation of children above 14 years of age with measles, mumps, rubella (MMR) vaccine and to introduce supplemental immunisation activities.
Subject(s)
Disease Outbreaks/statistics & numerical data , Rubella/epidemiology , Adolescent , Adult , Bosnia and Herzegovina/epidemiology , Child , Child, Preschool , Female , Humans , Incidence , Male , Population Surveillance , Risk Assessment/methods , Risk Factors , Young AdultABSTRACT
This report concerns the detection of two abnormal hemoglobins (Hb), Hb Manitoba and Hb G Coushatta, during analysis for glycohemoglobin (Hb A1c). Blood samples from two diabetic patients, analyzed for Hb A1c by HPLC, were found to contain additional Hb peaks. The presence of an abnormal Hb was confirmed in both instances by hemoglobinopathy studies. Structural studies determined the two Hb variants to be Hb Manitoba (alpha 2 102 Ser----Arg beta 2) and Hb G-Coushatta (alpha 2 beta 2 22 Glu----Ala). The significance of the presence of an abnormal Hb in Hb A1c analysis is discussed.
Subject(s)
Glycated Hemoglobin/analysis , Hemoglobins, Abnormal/analysis , Amino Acid Sequence , Chromatography, High Pressure Liquid , Diabetes Mellitus/blood , Electrophoresis, Cellulose Acetate , Hemoglobins, Abnormal/genetics , Humans , Middle Aged , Molecular Sequence Data , Peptide Mapping , TrypsinABSTRACT
The molecular weights of two active principles extracted from the urophysis of the teleost fish Catostomus commersoni in 0.1 N HC1 or in 0.25% acetic acid have been investigated by gel filtration chromatography and SDS-polyacrylamide gel electrophoresis. Two peptides with urotensin I Tlong-acting rat hypotensive) activity and two peptides with urotensin II (fish smooth muscle stimulating) activity were found by these procedures. The smaller of the two urotensin I peptides (molecular weight 1200-1700), designated urotensin Is, was shown to be a fragment of the larger peptide (molecular weight 2300-3000) which is produced by acid hydrolysis withour loss of rat hypotensive activity. The two urotensin II peptides are suggested to represent either a monomer and a dimer or open and closed forms of a peptide.
