ABSTRACT
A dilemma for corrections practitioners is treatment for pretrial detainees. They are innocent until proven guilty and are not required to take treatment, but many may benefit from intervention. To assess the general level of treatment interest and potential differences, a sample of 221 male remand and sentenced Canadian provincial prisoners completed several Client Evaluation of Self and Treatment (CEST) scales. Prisoner treatment motivation and its correlates were assessed by examining univariate, bivariate, and multivariate effects for demographic attributes, legal factors, risk, perceptions of personal/family/pressure for treatment, and depression. It was found that about 36% to 40% of study subjects expressed moderate to strong motivation for treatment. Age, pressure, and depression were the only correlates consistently associated with treatment motivation. There were no differences found between remand and sentenced prisoners. Results indicated that pretrial detainees have a definite interest in undertaking programming.
Subject(s)
Motivation , Prisoners/classification , Prisoners/psychology , Substance-Related Disorders/therapy , Adult , Age Factors , Canada , Depression , Diagnostic Self Evaluation , Humans , Interpersonal Relations , Male , Reproducibility of Results , Self-AssessmentABSTRACT
APEX is an engineered peroxidase that catalyzes the oxidation of a wide range of substrates, facilitating its use in a variety of applications from subcellular staining for electron microscopy to proximity biotinylation for spatial proteomics and transcriptomics. To further advance the capabilities of APEX, we used directed evolution to engineer a split APEX tool (sAPEX). A total of 20 rounds of fluorescence activated cell sorting (FACS)-based selections from yeast-displayed fragment libraries, using 3 different surface display configurations, produced a 200-amino-acid N-terminal fragment (with 9 mutations relative to APEX2) called "AP" and a 50-amino-acid C-terminal fragment called "EX". AP and EX fragments were each inactive on their own but were reconstituted to give peroxidase activity when driven together by a molecular interaction. We demonstrate sAPEX reconstitution in the mammalian cytosol, on engineered RNA motifs within a non-coding RNA scaffold, and at mitochondria-endoplasmic reticulum contact sites.
