Subject(s)
Factor VII Deficiency/genetics , Factor VII , Factor XI Deficiency/genetics , Factor XI , Adult , Blood Coagulation Tests , DNA Mutational Analysis , Enzyme-Linked Immunosorbent Assay , Factor VII Deficiency/complications , Factor XI Deficiency/complications , Family Health , Female , Humans , Male , Mutation , PregnancyABSTRACT
BACKGROUND: Efficacy and safety of an alpha-tocopherol-enriched emulsion incorporating soybean, coconut, olive, and fish oils (SMOF) are compared in terms of biologic parameters to those of soybean oil-based emulsion (LIPOVEN). METHODS: Twenty stressed patients were randomly assigned in a double-blind study to receive at least a 5-day course of total parenteral nutrition. Plasma activities of liver enzymes, C-reactive protein, antioxidant capacity, alpha-tocopherol, retinol, and low density lipoprotein (LDL)-alpha-tocopherol levels were determined. LDL-lipid oxidation is measured after incubation of the LDL in the presence of a prooxidant. RESULTS: The plasma activities of liver enzymes and the phospholipids/apo A1 ratio were increased in both groups. However, in the SMOF group the increases were lower than in the LIPOVEN group and non-significant for the CRP plasma level and the alanineamino-transferase activity. Before parenteral nutrition, the plasma antioxidant status was markedly reduced in both groups. After parenteral nutrition discontinuation, the antioxidant capacity and the amount of LDL-derived oxidation by-products formed were comparable in both groups. There was a significant improvement in plasma lipophilic antioxidant vitamins and LDL-alpha-tocopherol levels only in the SMOF group. CONCLUSIONS: The lower increase of plasma liver enzymes and phospholipids/apo A1 ratio in the SMOF group suggest a better liver function than in the LIPOVEN group. This beneficial effect results in a higher liver mobilization and plasma levels of lipophilic antioxidants. They could, together with higher delivery of omega-3 fatty acids to peripheral tissues, contribute positively to survival rate of stressed patients.
Subject(s)
Antioxidants/metabolism , Fat Emulsions, Intravenous/chemistry , Liver/drug effects , Liver/physiology , Parenteral Nutrition, Total/methods , Aged , Alanine Transaminase/metabolism , Apolipoprotein A-I/metabolism , C-Reactive Protein/analysis , Critical Care/methods , Double-Blind Method , Fat Emulsions, Intravenous/administration & dosage , Female , Fish Oils , Humans , Liver/enzymology , Male , Middle Aged , Olive Oil , Phospholipids/metabolism , Plant Oils , Postoperative Care , Prospective Studies , Safety , Soybean Oil , Thiobarbituric Acid Reactive Substances/analysis , Treatment Outcome , alpha-Tocopherol/administration & dosage , alpha-Tocopherol/bloodABSTRACT
In order to identify unknown mutations, the FAMA method was used to rapidly screen the fibrinogen chain genes in individuals with dysfibrinogenemias. Chemical cleavage at mismatches on heteroduplexes DNA end-labeled with strand-specific fluorescent dyes reliably detects sequence changes in DNA fragments of up to 1.5 kb and locates them precisely. This method was successfully used for the detection of three new dysfibrinogenemias: Poissy III, Tahiti (heterozygous Aalpha Arg16His) and Saint-Germain I (heterozygous AalphaGly12Val). The mutations were confirmed by dideoxy sequencing.
