ABSTRACT
It is shown that a short incubation of peripheral human lymphocytes with PGE2 is able to reduce the B cell differentiation induced by PWM. The target of PGE2 action appears to belong to T lymphocytes, since the treatment of non-T cells is uneffective in reducing the immune response. Both OKT4+ and OKT8+ subsets are sensitive to PGE2. Data concerning the role of endogenous as well as exogenous PGE2 either on unfractionated or fractionated OKT4+, OKT8+ and non-T lymphocytes are also discussed. The PGE2 inhibition on immunoglobulin synthesis in PWM-stimulated cultures seems to be mediated by a complex effect on both the T cell subsets.
Subject(s)
Dinoprostone/pharmacology , Lymphocyte Activation/drug effects , Antibody-Producing Cells/drug effects , Antibody-Producing Cells/immunology , Antigens, Differentiation, T-Lymphocyte , B-Lymphocytes/drug effects , B-Lymphocytes/immunology , CD4 Antigens , CD8 Antigens , Cell Differentiation/drug effects , Humans , In Vitro Techniques , Pokeweed Mitogens/pharmacology , T-Lymphocytes/drug effects , T-Lymphocytes/immunologyABSTRACT
A short preincubation of human T and B lymphocyte subpopulations with physiologic or pharmacologic concentrations of PGs E2 and F1 alpha, but not with E1 and F2 alpha, markedly depresses the cell ability to bind immune complexes, through FcR-IgG. This effect appears to be relatively temperature-independent. These observations indicate that PG treatment of human lymphocytes may be useful to distinguish the subclasses of FcR-IgG-bearing T and B cells, which are sensible to the modulating effect of PGs.
