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J Parasitol ; 107(2): 267-274, 2021 03 01.
Article in English | MEDLINE | ID: mdl-33784742

ABSTRACT

The hawksbill turtle Eretmochelys imbricata is a critically endangered species with a worldwide distribution. Limited information is available about the naturally occurring intestinal parasites of this species and what impact these parasites may have on the health of the hawksbill turtle. Diaschistorchis pandus was identified postmortem in 5 hawksbill turtles from Grenada, West Indies, using morphologic characterization. Sanger sequencing was performed for conserved ribosomal regions (5.8S, ITS2, 28S) and the mitochondrial cytochrome c oxidase subunit 1 gene (COI). Phylogenetic analysis of the 28S rRNA gene sequence data shows D. pandus clustering with other trematodes in the family Pronocephalidae, corroborating morphological classification. No genetic sequences have been previously reported for this trematode species, which has limited the collection of objective epidemiological data about this parasite of marine turtles.


Subject(s)
Trematoda/classification , Trematode Infections/veterinary , Turtles/parasitology , Animals , Autopsy/veterinary , DNA, Helminth/chemistry , DNA, Helminth/genetics , Endangered Species , Grenada , Intestine, Small/parasitology , Intestine, Small/pathology , Male , Phylogeny , RNA, Ribosomal, 28S/genetics , Trematoda/anatomy & histology , Trematoda/genetics , Trematoda/isolation & purification , Trematode Infections/parasitology
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