ABSTRACT
A long-term cryopreservation study of vegetative cells of Micrococcus lutea ATCC 9341a, Micrococcus lutea ATCC 15957, and Staphylococcus epidermidis ATCC 12228 cells, used in our antibiotic bioassay procedure, was conducted. The cryoprotective abilities of 1% methylcellulose solution and a 15% glycerol solution at -14 degrees C were determined. More organisms remained viable in 1% methylcellulose than in 15% glycerol. Overall survival of Staphylococcus epidermidis ATCC 12228 after 365 days was 1.5 logs lower than the other 2 organisms. The sensitivity and the resistance of the preserved organisms to various antibiotics did not change. The methodology is simple and inexpensive, saves analytical time, and avoids risk of contamination and sudden loss of a well-characterized culture.
Subject(s)
Anti-Bacterial Agents/pharmacology , Cryopreservation , Micrococcus luteus , Staphylococcus epidermidis , Bacteriological Techniques , Cryopreservation/methods , Glycerol , Methylcellulose , Microbial Sensitivity Tests , Time FactorsSubject(s)
Cattle/metabolism , Drug Residues/analysis , Kidney/metabolism , Neomycin/analysis , Administration, Oral , Animals , Biological Assay/veterinary , Chromatography, High Pressure Liquid/veterinary , Dose-Response Relationship, Drug , Drug Residues/pharmacokinetics , Female , Kidney/chemistry , Male , Neomycin/administration & dosage , Neomycin/pharmacokinetics , Reference Standards , United States , United States Food and Drug AdministrationABSTRACT
An iron-inefficient variety of peanut plant, when grown hydroponically with the catechol siderophore of Rhizobium (peanut isolate), showed increased growth and chlorophyll content compared with plants grown with Fe alone. The siderophore, when used in concentrations less than the concentrations of Fe, was still effective in grwoth promotion, indicating that it might function as a shuttle agent, solubilizing and supplying Fe to the plant. Similar results were also obtained with desferrioxamine B.
ABSTRACT
The effects of temperature and of ultraviolet radiation on the multiplication of bacteriophage phi29 were studied. Samples of phi29 that had been irradiated to surviving fractions of 0.44 or 0.10 were propagated at 37 degrees C, 42 degrees C and 43.5 degrees C. Latent periods and burst sizes were obtained from one-step growth curves. At a particular temperature, as the dose delivered to the virus was increased, the latent period was extended and the burst size was decreased. For unirradiated virus, the burst size was the same at 42 degrees C as at 37 degrees C, but decreased dramatically at 43.5 degrees C. For virus subjected to a particular dose, the burst size decreased as the temperature was raised. A statistical technique for improving the reliability of parameters obtained from one-step growth curves is presented.
