Challenges and opportunities for SERS in the infrared: materials and methods.

In the wake of a global, heightened interest towards biomarker and disease detection prompted by the SARS-CoV-2 pandemic, surface enhanced Raman spectroscopy (SERS) positions itself again at the forefront of biosensing innovation. But is it ready to move from the laboratory to the clinic? This review presents the challenges associated with the application of SERS to the biomedical field, and thus, to the use of excitation sources in the near infrared, where biological windows allow for cell and through-tissue measurements. Two main tackling strategies will be discussed: (1) acting on the design of the enhancing substrate, which includes manipulation of nanoparticle shape, material, and supramolecular architecture, and (2) acting on the spectral collection set-up. A final perspective highlights the upcoming scientific and technological bets that need to be won in order for SERS to stably transition from benchtop to bedside.

Carbon nano dot decorated copper nanowires for SERS-Fluorescence dual-mode imaging/anti-microbial activity and enhanced angiogenic activity.

Copper nanoparticles are explored significantly for their antimicrobial activity, especially for antibiotic-resistant strain infections. However, copper has severe toxic responses and mostly it is due to its generation capability of reactive oxygen species (ROS) molecules while interacting with in vitro or in vivo systems. In the current study, wire shaped copper nanostructures were synthesized via microwave irradiation with single step doping of carbon nanodots (CDs). The synthesized material (CuCs) was characterized by UV-Vis spectroscopy, fluorescence spectroscopy, FTIR, TEM, FESEM, XRD, DLS, and XPS. The fluorescence spectroscopy, microscopy and Raman spectroscopy results suggested CuCs to work well as a bi-modal imaging nanoprobe (fluorescence/SERS). The cell culture studies prove significant cytocompatibility and ROS scavenging property of the samples with respect to control. Further, CuCs-gelatin nanocomposite thin films were prepared and implanted into rodent deep wound model. The histological study has showed enhanced angiogenesis in the subcutaneous region. The results were validated by immuno-histochemistry. The ROS scavenging and enhanced angiogenesis were validated via gene expression studies and a HIF-α induced enhanced angiogenesis mechanism was also proposed for better wound healing.

Solar degradation of diclofenac using Eosin-Y-activated TiO2: cost estimation, process optimization and parameter interaction study.

Diclofenac (DCF), a widely used non-steroidal anti-inflammatory drug, is a commonly detected substance that readily accumulates in tissues of aquatic fish and poses a threat to wildlife and freshwater quality. Advanced Oxidation Processes have been employed as an alternative due to the inadequacy of conventional treatment methods of trace contaminants. This study utilized an innovative method of solar-activation of TiO2 using Eosin-Y dye for the degradation of DCF. Furthermore, the study incorporated a central composite design (CCD) to optimize the dye concentration and estimated the cost for the present process. Optimized parameters for light intensity (750 mW/cm2), Eosin-Y dye concentration (2 mg/L), TiO2 loading (37.5 mg/cm2) and DCF concentration (25 mg/L) were determined through a CCD. The optimized parameters convey a DCF degradation rate of 40% and 49% for 2 ppm (low range) and 4 ppm (high range) dye concentrations, respectively, for a 5-minute reaction time. Cost estimation for the materials used was for the current process was also performed. It was determined that the additional cost of using 4 ppm instead of 2 ppm to achieve only 10% more DCF degradation is not warranted and would require additional treatment to remove subsequently formed halogenated compounds.