Efficacy of Annona glabra extract against acute hepatopancreatic necrosis disease in white-leg shrimp (Penaeus vannamei).

This study aims to investigate the use of pond apple (Annona glabra) compounds as a novel strategy to prevent and treat acute hepatopancreatic necrosis disease (AHPND) as well as to better understand the mechanism of health improvement in shrimp. The A. glabra leaf extracts were extracted using various solvents and examined for in vitro and in vivo activity against Vibrio parahaemolyticus strains. In comparison with ethanol and water extracts, methanol extract showed the strongest bactericidal effect (MBC/MIC ratio of 2.50 ± 1.00), with minimal inhibitory concentration (MIC) of 0.023 ± 0.012 mg ml-1 and minimum bactericidal concentration (MBC) of 0.065 ± 0.062 mg ml-1. White leg shrimp (P. vannamei, body weight 10.37 ± 0.27 g) fed A. glabra methanol extracts-containing diets (AMEDs) at 1 %, 1.5 %, and 2.0 % demonstrated no deleterious effects on survival and were significantly increased in length and weight after 30 days of feeding. The level of total haemocyte, hyaline haemocyte on day 15 and granulocyte on day 30 remarkably increased (p < 0.05) in shrimps fed AMEDs groups compared to those in the control group. The finding demonstrates that granulocyte was induced time dependently. In particular, the survival rate of V. parahaemolyticus challenged shrimps under medication with AMEDs at 1.5 % and 2.0 % was significantly higher (p < 0.05) than that of the control group. The decrease in bacterial load of Vibrio spp. and V. parahaemolyticus was obviously recorded in hepatopancreas shrimp given AMEDs 1.5 % and 2.0 % and may be linked to herb characteristics such as antibacterial activity, enhancing innate immunity, and its potential to maintain the integrity of hepatopancreatic tissue. Our findings suggest that A. glabra extract might be used as a health enhancer in commercial farmed shrimp.

Detection of prevalent SARS-CoV-2 variant lineages in wastewater and clinical sequences from cities in Quebec, Canada

Wastewater-based epidemiology has emerged as a promising tool to monitor pathogens in a population, particularly when clinical diagnostic capacities become overwhelmed. During the ongoing COVID-19 pandemic caused by Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2), several jurisdictions have tracked viral concentrations in wastewater to inform public health authorities. While some studies have also sequenced SARS-CoV-2 genomes from wastewater, there have been relatively few direct comparisons between viral genetic diversity in wastewater and matched clinical samples from the same region and time period. Here we report sequencing and inference of SARS-CoV-2 mutations and variant lineages (including variants of concern) in 936 wastewater samples and thousands of matched clinical sequences collected between March 2020 and July 2021 in the cities of Montreal, Quebec City, and Laval, representing almost half the population of the Canadian province of Quebec. We benchmarked our sequencing and variant-calling methods on known viral genome sequences to establish thresholds for inferring variants in wastewater with confidence. We found that variant frequency estimates in wastewater and clinical samples are correlated over time in each city, with similar dates of first detection. Across all variant lineages, wastewater detection is more concordant with targeted outbreak sequencing than with semi-random clinical swab sampling. Most variants were first observed in clinical and outbreak data due to higher sequencing rate. However, wastewater sequencing is highly efficient, detecting more variants for a given sampling effort. This shows the potential for wastewater sequencing to provide useful public health data, especially at places or times when sufficient clinical sampling is infrequent or infeasible.

Preliminary Result of Arthroscopic Anterior Cruciate Ligament Reconstruction Using Anterior Half of Peroneus Longus Tendon Autograft.

BACKGROUND: Arthroscopic anterior cruciate ligament reconstruction is one of the most successful operations in sports medicine. At present, ligament autografts have been the best method due to good histocompatibility, rapid healing, no cross-contamination, and low cost of treatment. However, autografts do not have infinite amount and are also not always feasible. Anterior half of peroneus longus tenden autograft is likely to become a source of autograft with many advantages. This study aims to evaluate the clinical outcomes of arthroscopic anterior cruciate ligament reconstruction using anterior half of peroneus longus tendon autograft (AHPLT). AIM: To evaluate the initial outcome of ACL reconstruction arthroscopy by anterior half of peroneus longus tendon. METHODS: This is a prospective non-controlled case series. RESULTS: A prospective study on 30 patients (from 9 / 2016 to 01 / 2019) had both ACL and MCL injury who had operated ACL reconstruction using anterior half of peroneus longus tendon autograft (AHPLT) at Department of General Orthopaedic and Trauma, Viet Duc hospital. Our outcome: the year average 35.4 ys, the rate of ACL rupture combined with meniscus injury was 40%. The average diameter AHPLT autograft is 7.0 mm. The function Lysholm scores improved from 59 to 94.27 postoperative 6 months. No difference beetwen the AOFAS scale of preoperative and postoperative. CONCLUSION: Peroneus longus tendon is recommended to be a safe and practical autograft resource for arthroscopic anterior cruciate ligament reconstruction.