ABSTRACT
INTRODUCTION: To evaluate the outcomes of retrograde intrarenal surgery (RIRS) and ultra-mini percutaneous nephrolithotomy (umPCNL) in the management of renal calculi. MATERIAL AND METHODS: Between March 2015 and January 2018, a total of 44 patients were treated with umPCNL. The outcomes of these patients were compared with 75 patients who underwent RIRS for renal calculi during the same time period. RESULTS: Median stone size was 9 mm in the umPCNL group and 7 mm in the RIRS group. Stone-free rates after a single procedure were achieved in 85% of patients for the RIRS group and 98% for the umPCNL group. 16% of RIRS patients were left with a ureteric stent, whilst 7% of patients (n = 5) needed a second RIRS. One patient in the umPCNL group was left with a percutaneous nephrostomy; all other patients were left totally tubeless. The mean operative time was 66 minutes in the RIRS group and 55 minutes in the umPCNL group (p = 0.04). The minor complication rates for the RIRS and umPCNL groups were 17% and 15%, respectively. One patient in the RIRS group required postoperative nephrostomy insertion; there were no major complications in the umPCNL group. The median length of stay was 0 days in the RIRS group and 1 day in the umPCNL group. CONCLUSIONS: The overall study showed that umPCNL has low complication rates and good stone-free rates, with a lower requirement for ancilliary procedures. UmPCNL is an acceptable alternative in selected patients with small- to moderate-sized renal calculi.
ABSTRACT
Diabetes mellitus is associated with sensory abnormalities, including exacerbated responses to painful (hyperalgesia) or non-painful (allodynia) stimuli. These abnormalities are symptoms of diabetic peripheral neuropathy (DPN), which is the most common complication that affects approximately 50% of diabetic patients. Yet, the underlying mechanisms linking hyperglycemia and symptoms of DPN remain poorly understood. The transient receptor potential vanilloid 1 (TRPV1) channel plays a central role in such sensory abnormalities and shows elevated expression levels in animal models of diabetes. Here, we investigated the function of TRPV1 channels in sensory neurons cultured from the dorsal root ganglion (DRG) of neonatal mice, under control (5mM) and high glucose (25mM) conditions. After maintaining DRG neurons in high glucose for 1 week, we observed a significant increase in capsaicin (CAP)-evoked currents and CAP-evoked depolarizations, independent of TRPV1 channel expression. These functional changes were largely dependent on the expression of the receptor for Advanced Glycation End-products (RAGE), calcium influx, cytoplasmic ROS accumulation, PKC, and Src kinase activity. Like cultured neurons from neonates, mature neurons from adult mice also displayed a similar potentiation of CAP-evoked currents in the high glucose condition. Taken together, our data demonstrate that under the diabetic condition, DRG neurons are directly affected by elevated levels of glucose, independent of vascular or glial signals, and dependent on RAGE expression. These early cellular and molecular changes to sensory neurons in vitro are potential mechanisms that might contribute to sensory abnormalities that can occur in the very early stages of diabetes.
Subject(s)
Diabetic Neuropathies/metabolism , Ganglia, Spinal/metabolism , Glucose/pharmacology , Long-Term Potentiation/drug effects , Receptor for Advanced Glycation End Products/metabolism , Sensory Receptor Cells/metabolism , TRPV Cation Channels/metabolism , Animals , Calcium Signaling/drug effects , Calcium Signaling/genetics , Capsaicin/pharmacology , Diabetic Neuropathies/genetics , Diabetic Neuropathies/pathology , Diabetic Neuropathies/physiopathology , Evoked Potentials/drug effects , Evoked Potentials/genetics , Ganglia, Spinal/pathology , Ganglia, Spinal/physiopathology , Glucose/metabolism , Humans , Long-Term Potentiation/genetics , Mice , Mice, Knockout , Reactive Oxygen Species/metabolism , Receptor for Advanced Glycation End Products/genetics , Sensory Receptor Cells/pathology , TRPV Cation Channels/geneticsABSTRACT
Respiratory syncytial virus (RSV) causes serious respiratory illness in infants and elderly. RSV infection induces short-lived immunity, which leaves people prone to re-infection. In contrast, the RSV fusion (F) protein formulated with a novel adjuvant (∆F/TriAdj) elicits long term protective immunity. A comparison of RSV-immunized mice to mice vaccinated with a single dose of ∆F/TriAdj showed no difference in IgG1 and IgG2a production; however, local IgA secreting memory B cell development and B cell IgA production were significantly lower in RSV vaccinated mice than in ∆F/TriAdj-immunized mice. This indicates a potential reason as to why long-term immunity is not induced by RSV infection. The comparison also revealed that germinal center lymphocyte populations were higher in ∆F/TriAdj-vaccinated mice. Furthermore, ∆F/TriAdj induced higher gene expression of activation-induced cytidine deaminase (AID), as well as IL-6, IL-21, TGF-ß cytokines, which are key players in IgA class switch recombination, ultimately leading to a sustained long-term memory response.
