Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters










Database
Language
Publication year range
1.
Neuroscience ; 172: 94-103, 2011 Jan 13.
Article in English | MEDLINE | ID: mdl-20974231

ABSTRACT

Activation of the dopaminergic (DA) neurons of the ventral tegmental area (VTA) by ethanol has been implicated in its rewarding and reinforcing effects. We previously demonstrated that ethanol enhances GABA release onto VTA-DA neurons via activation of 5-HT2C receptors and subsequent release of calcium from intracellular stores. Here we demonstrate that excitation of VTA-DA neurons by ethanol is limited by an ethanol-enhancement in GABA release. In this study, we performed whole-cell voltage clamp recordings of miniature inhibitory postsynaptic currents (mIPSCs) and cell-attached recordings of action potential firing from VTA-DA neurons in midbrain slices from young Long Evans rats. Acute exposure to ethanol (75 mM) transiently enhanced the firing rate of VTA-DA neurons as well as the frequency of mIPSCs. Simultaneous blockade of both GABA(A) and GABA(B) receptors (Picrotoxin (75 µM) and SCH50911 (20 µM)) disinhibited VTA-DA firing rate whereas a GABA(A) agonist (muscimol, 1 µM) strongly inhibited firing rate. In the presence of picrotoxin, ethanol enhanced VTA-DA firing rate more than in the absence of picrotoxin. Additionally, a sub-maximal concentration of muscimol together with ethanol inhibited VTA-DA firing rate more than muscimol alone. DAMGO (3 µM) inhibited mIPSC frequency but did not block the ethanol-enhancement in mIPSC frequency. DAMGO (1 and 3 µM) had no effect on VTA-DA firing rate. Naltrexone (60 µM) had no effect on basal or ethanol-enhancement of mIPSC frequency. Additionally, naltrexone (20 and 60 µM) did not block the ethanol-enhancement in VTA-DA firing rate. Overall, the present results indicate that the ethanol enhancement in GABA release onto VTA-DA neurons limits the stimulatory effect of ethanol on VTA-DA neuron activity and may have implications for the rewarding properties of ethanol.


Subject(s)
Dopamine/physiology , Ethanol/pharmacology , Neurons/physiology , Synaptic Transmission/drug effects , Ventral Tegmental Area/drug effects , gamma-Aminobutyric Acid/physiology , Animals , Disease Models, Animal , Male , Neurons/drug effects , Organ Culture Techniques , Rats , Rats, Long-Evans , Synaptic Transmission/physiology , Ventral Tegmental Area/cytology , Ventral Tegmental Area/physiology
SELECTION OF CITATIONS
SEARCH DETAIL
...