ABSTRACT
We report on a flexible platform for molecular sensing in the terahertz range. Merging the well-established technologies of near-infrared electro-optic modulation and photomixing realizes a spectrally adaptable terahertz source, which is combined with a new generation of compact gas cells, so-called substrate-integrated hollow waveguides (iHWGs). iHWGs have been developed in the mid-infrared and provide flexibility in the design of the optical absorption path. Here, we demonstrate its suitability for the terahertz domain by presenting its low propagation losses and by measuring rotational transitions of nitrious oxide (N2O). A fast frequency sideband modulation technique results in substantially reduced measurement times and increased accuracy compared to a standard wavelength tuning method.
ABSTRACT
Broadband emission in the terahertz spectral region is a prerequisite for applications such as spectroscopy or white light sources. Appropriate signal powers and a compact design are advantageous for this use. A technology which meets these requirements are terahertz quantum cascade lasers. These electrically pumped, on-chip semiconductor lasers provide high output powers and the freedom of tailoring their emission wavelength by bandstructure engineering. By combining multiple active region designs emitting at different wavelengths in a single structure, one can obtain broadband emission from a single device. Here, we present a heterogeneous terahertz quantum cascade laser consisting of five individual active regions based on a three-well, LO-phonon depopulation design. The devices lase in pulsed and continuous-wave operation and emit in a spectral range from 1.9 to 4.5 THz, covering a bandwidth of 1.37 octaves. The use of the three-well design, which was optimized for high temperature operation, leads to a maximum operating temperature in the pulsed operation of 143 K.
ABSTRACT
We demonstrate a terahertz (THz) frequency comb that is flexible in terms of its frequency range and the number and spacing of comb lines. We use a combination of near-infrared laser diodes, phase modulation, and opto-electronic frequency conversion. The THz comb lines are characterized to be <10MHz by resolving the pressure-dependent collisional broadening of an ammonia molecule rotational mode.
ABSTRACT
Photodynamic therapy (PDT) involves a photosensitizing agent activated with light to induce cell death. Two-photon excited PDT (TPE-PDT) offers numerous benefits compared to traditional one-photon induced PDT, including an increased penetration depth and precision. However, the in vitro profiling and comparison of two-photon photosensitizers (PS) are still troublesome. Herein, we report the development of an in vitro screening platform of TPE-PS using a 3D osteosarcoma cell culture. The platform was tested using three different two-photon (2P) active compounds - a 2P sensitizer P2CK, a fluorescent dye Eosin Y, and a porphyrin derivative (TPP). Their 2P absorption cross-sections (σ2PA) were characterised using a fully automated z-scan setup. TPP exhibited a remarkably high σ2PA at 720 nm (8865 GM) and P2CK presented a high absorption at 850 nm (405 GM), while Eosin Y had the lowest 2P absorption at the studied wavelengths (<100 GM). The cellular uptake of PS visualized using confocal laser scanning microscopy showed that both TPP and P2CK were internalized by the cells, while Eosin Y stayed mainly in the surrounding media. The efficiency of the former two TPE-PS was quantified using the PrestoBlue metabolic assay, showing a significant reduction in cell viability after two-photon irradiation. The possibility of damage localization was demonstrated using a co-culture of adipose derived stem cells together with osteosarcoma spheroids showing no signs of damage to the surrounding healthy cells after TPE-PDT.
