ABSTRACT
A 21-bp element called glucocorticoid modulatory element (GME) modulates the glucocorticoid receptor-mediated responses via the binding of an as yet poorly characterized transacting complex of proteins containing the 88-kDa GMEB1 and the 67-kDa GMEB2. Using heat shock protein 27 (HSP27) as bait in the yeast two-hybrid assay, we cloned a 1.83-kb cDNA encoding a novel 573-amino acid protein called human GMEB1 (hGMEB1). hGMEB1 possesses a KDWK domain, contains sequences almost identical (36/38) to three tryptic peptides of rat GMEB1 and shares 38% identity with rat GMEB2. hGMEB1 is ubiquitously expressed as a 85-kDa protein in all cell lines and tissues examined. In vitro translated hGMEB1 bound specifically to GME oligonucleotides yielding a complex of similar size to the complex obtained using rat liver nuclear extracts. Both complexes were supershifted with an antibody specific to hGMEB1. Co-immunoprecipitation experiments confirmed the in vivo interaction of HSP27 with hGMEB1.
Subject(s)
Heat-Shock Proteins , Transcription Factors/metabolism , 3T3 Cells , Amino Acid Sequence , Animals , Binding Sites , Cell Line , Cloning, Molecular , Cricetinae , Glutathione Transferase/biosynthesis , HSP27 Heat-Shock Proteins , HeLa Cells , Humans , Mice , Molecular Chaperones , Molecular Sequence Data , Neoplasm Proteins/genetics , Neoplasm Proteins/metabolism , Oligodeoxyribonucleotides/chemistry , Oligodeoxyribonucleotides/metabolism , Protein Biosynthesis , Rats , Recombinant Fusion Proteins/biosynthesis , Recombinant Fusion Proteins/metabolism , Saccharomyces cerevisiae , Sequence Alignment , Sequence Homology, Amino Acid , Transcription Factors/chemistry , Transcription Factors/genetics , TransfectionABSTRACT
Vibration signatures of the Low Power Atmospheric Compensation Experiment satellite were obtained with a ground-based CO(2) laser radar. The laser radar operated in a cw mode and used autodyne receivers to extract relative target velocity information between a germanium retroreflector located at the base of the satellite and a retroreflector array located at the tip of an extended forward boom. Time-frequency analysis algorithms were applied to the vibration data to investigate the correlation between excitations and modal structure. The resultant analysis suggests that vibration modes of an on-orbit spacecraft can be suppressed with simple open-loop techniques.
ABSTRACT
Tomography is used to reconstruct 2-D images from 1-D range-resolved laser radar data. A doubled mode-locked Nd:YAG pulsed laser illuminates a conical object, and a receiver utilizing a streak camera resolves the reflected light in time.
